肺癌是我国发病率最高的肿瘤。重离子以其特有的倒转深度剂量分布和较高的相对生物学效应具有远优于常规射线的治疗效果。但是重离子与lncRNA的表达关系少有研究。申请者发现重离子辐射敏感的LncRNA PHL13。lncRNA PHL13过表达后肿瘤细胞存活率下降72%。此外还发现lncRNA PHL13抑制G-actin的聚合、影响PC4蛋白的核定位。因此提出LncRNA PHL13靶向Actin通路调控碳离子辐照诱导的细胞凋亡这一假说。.本项目拟采研究：.1、肺癌细胞的碳离子辐射敏感性与lncRNA PHL13表达的关系。.2、LncRNA PHL13通过与骨架蛋白的相互作用对细胞凋亡的影响。.3、LncRNA PHL13对小鼠肺癌移植瘤的影响。.本研究探讨lncRNA PHL13对Actin-PC4这一信号通路的调控机制，对于揭示重离子对肿瘤细胞的杀伤作用的机理有重要意义。

Lung cancer has the highest incidence and mortality rates in China. Heavy ions, with its unique inverted depth dose distribution and higher relative biological effectiveness, will be a better way for radiotherapy. But the relationship between the expression of lncRNA and heavy ions radiation is unclear.We found a heavy ion radiation sensitived LncRNA PHL13 and it’s overexpression caused tumor cells’ survival rate decreased for 72%. LncRNA PHL13 significantly inhibit polymerization of G-actin and nuclear localization of PC4 protein. Therefore, we hypothesized that lncRNA PHL13 regulates carbon ion induced apoptosis by targeting Actin-PC4 pathway..We intend to investigate:.1..The relationship between cell radiosensitivity and expression of lncRNA PHL13..2..LncRNA PHL13 regulates carbon ion induced apoptosis by targeting Actin..3..The effect of lncRNA PHL13 to xenograftsed lung cancer in mice..It’s quit important for revealing the mechanism of heavy ions’ lethal effects to tumor cells.