Development of the first wireless near infrared spectroscopy device for preclinical monitoring of cerebral oxygenation

开发第一台用于临床前脑氧合监测的无线近红外光谱仪

• 批准号: 10174203
• 负责人: Zeinab Barati
• 金额: $ 5.5万
• 依托单位: BARATI MEDICAL, LLC
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-15 至 2020-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10174203
• 关键词: Acute; Adult; Anesthesia procedures; Animal Model; Animals; Behavioral; Biomedical Research; Biotechnology; Blood flow; Brain; Brain Infarction; Brain Injuries; Brain Ischemia; Cause of Death; Cerebral Infarction; Cerebral cortex; Cerebrovascular Circulation; Clinical Trials; Data; Development; Devices; Evaluation; Failure; Feedback; Fiber Optics; Fibrinolytic Agents; Goals; Gold; Hour; Image; Imaging Device; Infarction; Ischemia; Ischemic Stroke; Knowledge; Laser-Doppler Flowmetry; Letters; Licensing; Light; Linear Regressions; Liquid substance; Location; Longitudinal Studies; Magnetic Resonance Imaging; Measurement; Measures; Medical; Middle Cerebral Artery Occlusion; Modeling; Monitor; Mus; Near-Infrared Spectroscopy; Neurologic; Neuroprotective Agents; Noise; Optics; Oryctolagus cuniculus; Oxyhemoglobin; Penetration; Pharmaceutical Preparations; Phase; Pre-Clinical Model; Procedures; Property; Rattus; Reaction; Reperfusion Therapy; Research; Secure; Signal Transduction; Skin; Small Business Innovation Research Grant; Source; Stroke; System; Technology; Testing; Therapeutic; Time; Tissues; Translations; Treatment Efficacy; United States; Visualization; Wireless Technology; Work; absorption; base; cerebral oxygenation; cost; cost effective; data acquisition; deoxyhemoglobin; disability; drug candidate; feature extraction; improved; in vivo; innovation; instrument; ischemic injury; male; miniaturize; minimally invasive; non-invasive monitor; optical imaging; pre-clinical; preclinical efficacy; preclinical study; prototype; sensor; stroke model; technological innovation; tool; user-friendly

PROJECT SUMMARY Stroke is the fifth largest cause of death and a leading cause of long-term disability among adults in the United States. More than 300 neuroprotective and thrombolytic drug candidates for ischemic stroke have failed in clinical trials between 1995 and 2015, raising concerns regarding the efficacy of preclinical studies. Our long- term goal is to improve the value of animal models of ischemic stroke and accelerate the path of the most efficacious treatments to clinical trials with reduced failure rates and associated costs. The goal of this SBIR proposal is to demonstrate the utility of a wireless optical imaging instrument for providing an in vivo estimate of brain ischemia. Such knowledge will reduce inconsistency in animal models of ischemic stroke and enable longitudinal assessment of treatments’ efficacy. At Barati Medical, LLC, we have developed a miniaturized, wireless near infrared spectroscopy (NIRS) prototype for noninvasive monitoring of cerebral oxygenation parameters, i.e. deoxyhemoglobin (Hb) and oxyhemoglobin (HbO2), in rats. In a preliminary study, our prototype detected a twofold reduction in cerebral oxygenation during deep anesthesia relative to light anesthesia in a male rat. The product of this proposal will be a cost-effective, miniaturized, wireless NIRS platform for the measurement of cerebral oxygenation in small lab animals, which enables in vivo, longitudinal assessment of brain injury models and treatments’ efficacy. Our innovation centers on the utility of a cost-effective NIRS solution in predicting the infarct volume after procedures of middle cerebral artery occlusion (MCAO) in freely moving animals. No other in vivo measure is readily available that accurately predicts infarct volume or behavioral deficit. Our Specific Aims are to refine the NIRS prototype, demonstrate the feasibility of the NIRS device in predicting behavioral deficits and infarct volume at 48h after ischemic stroke, and show the feasibility of continuous, long- term measurement of cerebral oxygenation during the subacute phase of ischemic stroke in rats.

项目摘要 中风是统一成年人长期残疾的第五大死亡原因，也是统一长期残疾的主要原因 国家。超过300多个缺血性中风的神经保护性和溶栓药候选者失败了 1995年至2015年之间的临床试验引起了人们对临床前研究有效性的关注。我们的长期 术语目标是提高缺血性中风动物模型的价值，并加速最多的路径 有效治疗失败率和相关成本降低的临床试验。这个Sbir的目标 建议是证明无线光学成像工具的实用性，以提供体内估计值 脑缺血。这种知识将减少缺血性中风动物模型的不一致并启用 治疗效率的纵向评估。 在Barati Medical，LLC，我们在红外光谱（NIRS）附近开发了一个微型的无线化。 用于非侵入性监测脑充氧参数的原型，即脱氧血红蛋白（HB）和 大鼠中的氧脱血红蛋白（HBO2）。在一项初步研究中，我们的原型检测到脑的降低双重降低 与雄性大鼠的轻度麻醉有关的深麻醉期间的氧合。该提议的产品将 成为一个经济高效的，微型的无线NIRS平台，用于测量小脑充氧 实验室动物，可以使体内，脑损伤模型的纵向评估以及治疗效率。 我们的创新集中于一种具有成本效益的NIR解决方案的实用性，以预测后的梗塞量 自由移动动物中脑动脉阻塞（MCAO）的过程。没有其他体内测量是 可以准确预测梗塞量或行为防御的可用。 我们的具体目的是完善NIRS原型，证明了NIRS设备预测的可行性 行为将缺血性中风后48h定义和梗塞体积，并显示了连续，长期的可行性 在大鼠缺血性中风的亚急性期间的脑充氧的期限测量。

项目成果

Near infrared spectroscopy detection of hemispheric cerebral ischemia following middle cerebral artery occlusion in rats.

近红外光谱检测大鼠大脑中动脉闭塞后半球脑缺血的情况

• DOI: 10.1016/j.neuint.2022.105460
• 发表时间: 2023
• 期刊: Neurochemistry international
• 影响因子: 4.2
• 作者: Wong,Ardy;Bhuiyan,MohammadIqbalHossain;Rothman,Jeffrey;Drew,Kelly;Pourrezaei,Kambiz;Sun,Dandan;Barati,Zeinab
• 通讯作者: Barati,Zeinab