Development of the first wireless near infrared spectroscopy device for preclinical monitoring of cerebral oxygenation

开发第一台用于临床前脑氧合监测的无线近红外光谱仪

• 批准号: 10174203
• 负责人: Zeinab Barati
• 金额: $ 5.5万
• 依托单位: BARATI MEDICAL, LLC
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-15 至 2020-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10174203
• 关键词: Acute; Adult; Anesthesia procedures; Animal Model; Animals; Behavioral; Biomedical Research; Biotechnology; Blood flow; Brain; Brain Infarction; Brain Injuries; Brain Ischemia; Cause of Death; Cerebral Infarction; Cerebral cortex; Cerebrovascular Circulation; Clinical Trials; Data; Development; Devices; Evaluation; Failure; Feedback; Fiber Optics; Fibrinolytic Agents; Goals; Gold; Hour; Image; Imaging Device; Infarction; Ischemia; Ischemic Stroke; Knowledge; Laser-Doppler Flowmetry; Letters; Licensing; Light; Linear Regressions; Liquid substance; Location; Longitudinal Studies; Magnetic Resonance Imaging; Measurement; Measures; Medical; Middle Cerebral Artery Occlusion; Modeling; Monitor; Mus; Near-Infrared Spectroscopy; Neurologic; Neuroprotective Agents; Noise; Optics; Oryctolagus cuniculus; Oxyhemoglobin; Penetration; Pharmaceutical Preparations; Phase; Pre-Clinical Model; Procedures; Property; Rattus; Reaction; Reperfusion Therapy; Research; Secure; Signal Transduction; Skin; Small Business Innovation Research Grant; Source; Stroke; System; Technology; Testing; Therapeutic; Time; Tissues; Translations; Treatment Efficacy; United States; Visualization; Wireless Technology; Work; absorption; base; cerebral oxygenation; cost; cost effective; data acquisition; deoxyhemoglobin; disability; drug candidate; feature extraction; improved; in vivo; innovation; instrument; ischemic injury; male; miniaturize; minimally invasive; non-invasive monitor; optical imaging; pre-clinical; preclinical efficacy; preclinical study; prototype; sensor; stroke model; technological innovation; tool; user-friendly

PROJECT SUMMARY Stroke is the fifth largest cause of death and a leading cause of long-term disability among adults in the United States. More than 300 neuroprotective and thrombolytic drug candidates for ischemic stroke have failed in clinical trials between 1995 and 2015, raising concerns regarding the efficacy of preclinical studies. Our long- term goal is to improve the value of animal models of ischemic stroke and accelerate the path of the most efficacious treatments to clinical trials with reduced failure rates and associated costs. The goal of this SBIR proposal is to demonstrate the utility of a wireless optical imaging instrument for providing an in vivo estimate of brain ischemia. Such knowledge will reduce inconsistency in animal models of ischemic stroke and enable longitudinal assessment of treatments’ efficacy. At Barati Medical, LLC, we have developed a miniaturized, wireless near infrared spectroscopy (NIRS) prototype for noninvasive monitoring of cerebral oxygenation parameters, i.e. deoxyhemoglobin (Hb) and oxyhemoglobin (HbO2), in rats. In a preliminary study, our prototype detected a twofold reduction in cerebral oxygenation during deep anesthesia relative to light anesthesia in a male rat. The product of this proposal will be a cost-effective, miniaturized, wireless NIRS platform for the measurement of cerebral oxygenation in small lab animals, which enables in vivo, longitudinal assessment of brain injury models and treatments’ efficacy. Our innovation centers on the utility of a cost-effective NIRS solution in predicting the infarct volume after procedures of middle cerebral artery occlusion (MCAO) in freely moving animals. No other in vivo measure is readily available that accurately predicts infarct volume or behavioral deficit. Our Specific Aims are to refine the NIRS prototype, demonstrate the feasibility of the NIRS device in predicting behavioral deficits and infarct volume at 48h after ischemic stroke, and show the feasibility of continuous, long- term measurement of cerebral oxygenation during the subacute phase of ischemic stroke in rats.

项目摘要 中风是美国成年人的第五大死亡原因，也是导致长期残疾的主要原因。 States.超过300种用于缺血性卒中的神经保护和溶栓药物候选药物在临床试验中失败， 1995年至2015年期间的临床试验，引起了对临床前研究有效性的担忧。我们长久以来- 长期目标是提高缺血性脑卒中动物模型的价值，加速缺血性脑卒中的最佳治疗途径。 临床试验的有效治疗，降低失败率和相关成本。本SBIR的目标 一个建议是证明一个无线光学成像仪器提供体内估计的效用 脑缺血这些知识将减少缺血性中风动物模型的不一致性， 治疗效果的纵向评估。 在Barati Medical，LLC，我们开发了一种小型化的无线近红外光谱仪（NIRS）， 用于无创监测脑氧合参数的原型，即脱氧血红蛋白（Hb）和 氧合血红蛋白（HbO 2）。在一项初步研究中，我们的原型检测到大脑中的 在雄性大鼠中，深度麻醉相对于轻度麻醉期间的氧合。该提案的成果将 是一个具有成本效益的，小型化，无线NIRS平台，用于测量脑氧合在小 实验室动物，这使得在体内，脑损伤模型和治疗效果的纵向评估。 我们的创新集中在一个具有成本效益的NIRS解决方案在预测梗死体积后的效用 大脑中动脉闭塞（MCAO）的自由活动的动物。没有其他体内测量方法 准确预测梗死体积或行为缺陷。 我们的具体目标是完善近红外光谱仪的原型，证明近红外光谱仪在预测中的可行性， 缺血性卒中后48小时的行为缺陷和梗死体积，并显示了持续，长时间， 大鼠缺血性中风亚急性期脑氧合的长期测量。

项目成果

Near infrared spectroscopy detection of hemispheric cerebral ischemia following middle cerebral artery occlusion in rats.

近红外光谱检测大鼠大脑中动脉闭塞后半球脑缺血的情况

• DOI: 10.1016/j.neuint.2022.105460
• 发表时间: 2023
• 期刊: Neurochemistry international
• 影响因子: 4.2
• 作者: Wong,Ardy;Bhuiyan,MohammadIqbalHossain;Rothman,Jeffrey;Drew,Kelly;Pourrezaei,Kambiz;Sun,Dandan;Barati,Zeinab
• 通讯作者: Barati,Zeinab