Protein Crystallization Imager

项目摘要 该申请要求资金购买具有双光路的Formulatrix ROCK IMAGER® 2， 能够进行紫外（UV）和多荧光成像，以监测和捕获高通量 结晶筛选实验。新成像仪的主要应用将是加速 结晶筛选在机构先进技术核心（ATC）的高分子X射线 贝勒医学院（Baylor College of Medicine）岩石成像仪2将取代现有的成像 2008年购买的系统，制造商将不再维护或维修。新 成像仪将通过快速筛选蛋白质晶体来增强X射线核心的能力， 荧光标记的大分子复合物的晶体。除了七个主要用户外， 四名次要用户具有蛋白质结晶或使用X射线晶体学进行研究的经验， 表示有兴趣使用ROCK IMAGER 2通过大分子X射线进行结晶筛选 晶体学ATC。 除了斯坦福大学，德克萨斯医学中心（TMC）只有另外两个学术机构。 拥有成像系统，但核心工作人员无法使用这两种系统。所要求的文书将 由X射线核心容纳和管理，为蛋白质及其结晶提供主要服务。 与核酸、小分子或两者的功能复合物。对发现新药的需求不断增长 反映了学院的精准医学和转化研究的战略计划，其中健康 专业人员和基础研究人员聚集在一起，开发新的治疗方法， 疾病、糖尿病和肥胖症、男性避孕药、癌症和阿尔茨海默病。除了极大 新的成像仪增强了核心的工作流程，将推进用户的个人研究计划，并将 提供给TMC的所有研究人员，以帮助他们找到大分子的结构解决方案， 兴趣

PROJECT SUMMARY This application is requesting funds to purchase a Formulatrix ROCK IMAGER® 2 with Dual Light Path and capable of both ultraviolet (UV) and multi-fluorescence imaging to monitor and capture high-throughput crystallization screening experiments. The primary application of the new imager will be to accelerate crystallization screening in the institutional Advanced Technology Core (ATC) for Macromolecular X-ray Crystallography at Baylor College of Medicine (BCM). The ROCK IMAGER 2 will replace the existing imaging system that was purchased in 2008, and will be no longer maintained or serviced by the manufacturer. The new imager will enhance the capabilities of the X-ray Core by enabling the rapid screening of protein crystals and crystals of fluorescently-labeled macromolecular complexes. In addition to the seven Major Users, who are experienced with protein crystallization or use X-ray crystallography for their research, four Minor Users have expressed interest in using the ROCK IMAGER 2 for crystallization screening through the Macromolecular X-ray Crystallography ATC. Besides BCM, there are only two other academic institutions in the Texas Medical Center (TMC) possessing an imaging system, neither of which is available for use by Core Staff. The requested instrument will be housed and managed by the X-ray Core to provide major services for the crystallization of proteins and their functional complexes with nucleic acids, small molecules, or both. The growing demand to discover new drugs reflects the College’s strategic plans for precision medicine and translational research, where health professionals and basic researchers come together to develop new therapeutics against emerging infectious diseases, diabetes and obesity, male contraceptive, cancer, and Alzheimer's disease. In addition to greatly enhancing the Core's workflow, the new imager will advance the individual research programs of users and will be made available to all investigators in the TMC to help find a structure solution of their macromolecule of interest.