Protein Crystallization Imager

蛋白质结晶成像仪

• 批准号: 10176250
• 负责人: Sukyeong Lee
• 金额: $ 13.63万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10176250
• 关键词: Alzheimer' s Disease; Complex; Crystallization; Diabetes Mellitus; Emerging Communicable Diseases; Funding; Health Professional; Individual; Institution; Label; Light; Macromolecular Complexes; Male Contraceptive Agents; Malignant Neoplasms; Manufacturer Name; Medical center; Medicine; Minor; Monitor; Nucleic Acids; Obesity; Proteins; Rapid screening; Research; Research Personnel; Roentgen Rays; Services; Strategic Planning; Structure; Technology; Texas; Translational Research; X-Ray Crystallography; college; experience; experimental study; fluorescence imaging; imager; imaging system; instrument; interest; macromolecule; novel therapeutics; precision medicine; programs; screening; small molecule; ultraviolet

PROJECT SUMMARY This application is requesting funds to purchase a Formulatrix ROCK IMAGER® 2 with Dual Light Path and capable of both ultraviolet (UV) and multi-fluorescence imaging to monitor and capture high-throughput crystallization screening experiments. The primary application of the new imager will be to accelerate crystallization screening in the institutional Advanced Technology Core (ATC) for Macromolecular X-ray Crystallography at Baylor College of Medicine (BCM). The ROCK IMAGER 2 will replace the existing imaging system that was purchased in 2008, and will be no longer maintained or serviced by the manufacturer. The new imager will enhance the capabilities of the X-ray Core by enabling the rapid screening of protein crystals and crystals of fluorescently-labeled macromolecular complexes. In addition to the seven Major Users, who are experienced with protein crystallization or use X-ray crystallography for their research, four Minor Users have expressed interest in using the ROCK IMAGER 2 for crystallization screening through the Macromolecular X-ray Crystallography ATC. Besides BCM, there are only two other academic institutions in the Texas Medical Center (TMC) possessing an imaging system, neither of which is available for use by Core Staff. The requested instrument will be housed and managed by the X-ray Core to provide major services for the crystallization of proteins and their functional complexes with nucleic acids, small molecules, or both. The growing demand to discover new drugs reflects the College’s strategic plans for precision medicine and translational research, where health professionals and basic researchers come together to develop new therapeutics against emerging infectious diseases, diabetes and obesity, male contraceptive, cancer, and Alzheimer's disease. In addition to greatly enhancing the Core's workflow, the new imager will advance the individual research programs of users and will be made available to all investigators in the TMC to help find a structure solution of their macromolecule of interest.

项目总结

项目成果

Design, Synthesis, X-ray Crystallography, and Biological Activities of Covalent, Non-Peptidic Inhibitors of SARS-CoV-2 Main Protease.

• DOI: 10.1021/acsinfecdis.3c00565
• 发表时间: 2024-01
• 期刊: ACS infectious diseases
• 影响因子: 5.3
• 作者: M. Ashraf-Uz-Zaman;Teck Khiang Chua;Xin Li;Yuan Yao;Bala Krishna Moku;Chandra Bhushan Mishra;V. Avadhanula;P. Piedra;Yongcheng Song

An auto-inhibited state of protein kinase G and implications for selective activation.

• DOI: 10.7554/elife.79530
• 发表时间: 2022-08-05
• 期刊: ELIFE
• 影响因子: 7.7
• 作者: Sharma, Rajesh;Kim, Jeong Joo;Qin, Liying;Henning, Philipp;Akimoto, Madoka;VanSchouwen, Bryan;Kaur, Gundeep;Sankaran, Banumathi;MacKenzie, Kevin R.;Melacini, Giuseppe;Casteel, Darren E.;Herberg, Friedrich W.;Kim, Choel
• 通讯作者: Kim, Choel

Structural basis of impaired disaggregase function in the oxidation-sensitive SKD3 mutant causing 3-methylglutaconic aciduria.

在氧化敏感的SKD3突变体中分解酶函数受损的结构基础，导致3-甲基谷氨酸酸尿。

• DOI: 10.1038/s41467-023-37657-9
• 发表时间: 2023-04-11
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Lee, Sukyeong;Lee, Sang Bum;Sung, Nuri;Xu, Wendy W.;Chang, Changsoo;Kim, Hyun-Eui;Catic, Andre;Tsai, Francis T. F.
• 通讯作者: Tsai, Francis T. F.