Vectra Polaris microscope imaging system

Vectra Polaris 显微镜成像系统

• 批准号: 10175807
• 负责人: Mercedes Gonzalez-Juarrero
• 金额: $ 45.18万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10175807
• 关键词: Affect; Biological; Biological Markers; Cell Communication; Colorado; Computer software; Core Facility; Development; Disease; Flow Cytometry; Fluorescence; Formalin; Foundations; Funding; Genus Mycobacterium; Image; Imaging technology; Immune response; Immunofluorescence Immunologic; Immunohistochemistry; Immunologic Markers; Laboratory Research; Lighting; Machine Learning; Microscope; Microscopic; Microscopy; Mission; Nature; Noise; Photobleaching; Pre-Clinical Model; Research; Research Personnel; Safety; Sampling; Scanning; Signal Transduction; Slide; Specimen; Speed; Stains; Structure; System; Technology; Time; Tissue Sample; Tissues; Training; United States National Institutes of Health; Universities; Vaccines; base; chemotherapy; digital; imaging system; improved; liquid crystal polymer; microscopic imaging; mycobacterial; operation; quantitative imaging; spatial relationship; user-friendly; vaccine candidate

PROJECT SUMMARY This proposal seeks funding for a Vectra® Polaris™ Automated Quantitative Imaging System (Polaris™), a state- of-the-art multispectral imaging microscope to serve multiple NIH-supported projects within the Mycobacteria Research Laboratories (MRL) at Colorado State University (CSU). Our collective mission is to develop effective chemotherapies and vaccines for mycobacterial diseases affecting millions worldwide. The biological activity and safety profile of candidate vaccines and treatments can only be fully understood through characterizing the host immune response, primarily by evaluating large networks of immune biomarkers through flow cytometry and immunohistochemistry. MRL researchers are seeking to visualize the spatial expression of immune biomarkers by multiplex staining of intact tissue samples obtained from preclinical models of mycobacterial diseases. Until now, microscopic analysis of biomarkers through confocal and traditional immunofluorescence was drastically limited by autofluorescence in tissue (especially in formalin fixed and diseased tissue) and low signal-to-noise ratios. The Polaris™ system has a unique set of microscopic capabilities that combined with the inForm® workstation allows simultaneous identification and quantification of multiple targets and their spatial relationships in tissue sections and microarrays. A critical aspect of the PolarisTM multispectral technology is the ability to readily distinguish autofluorescence from positive biomarker signal, improving the signal-to-noise ratio substantially. This allows for a more sensitive and accurate analysis of samples and enables true cell-to-cell interactions to be revealed. The system is user-friendly, and its automated scanning system allows for high speed digital and whole-slide scanning between 10-40x in brightfield or fluorescence. The nature of the illumination system significantly decreases deleterious effects such as photobleaching and photodamage, and thus specimens can be imaged for longer periods of time than with, for example, a confocal microscope. Furthermore, the advanced machine-learning based approaches of the included software automatically segment and quantitate tissue structures. The Polaris™ system will be incorporated into our existing microscopy core facility in order to expand access to trainees and other research groups at CSU and in Northern Colorado, while prioritizing access for the major users. Incorporation into the foundational core will sustain long term operation and provide consistent oversight and training. In summary, this state-of-the-art imaging technology is of critical importance to development of chemotherapies and vaccines by the MRL and will add value to multiple other NIH-supported projects at CSU.

项目摘要 该提案寻求用于Vectra®Polaris™自动定量成像系统（Polaris™）的资金，这是一种状态 - the-art多光谱成像显微镜，可在分枝杆菌中为多个受NIH支持的项目服务 科罗拉多州立大学（CSU）的研究实验室（MRL）。我们的集体使命是发展有效 分枝杆菌疾病的化学疗法和疫苗影响全球数百万。生物活性和 候选疫苗和治疗的安全性只能通过表征宿主来完全理解 免疫反应，主要是通过流式细胞仪评估免疫生物标志物的大型网络 免疫组织化学。 MRL研究人员正在寻求通过 从分枝杆菌疾病的临床前模型获得的完整组织样品的多种染色。到目前为止， 通过共聚焦和传统免疫荧光对生物标志物的显微镜分析受到了极大的限制 组织（尤其是在福尔马林固定组织和解剖组织中）和低信噪比的自动荧光。这 Polaris™系统具有独特的微观功能，与Inform®工作站结合使用 简单识别和定量多个目标及其在组织部分中的空间关系 和微阵列。偏光师多光谱技术的一个关键方面是能够轻松区分的能力 来自正生物标志物信号的自动荧光，大大提高了信噪比。这允许 对样品的更敏感，更准确的分析，可以揭示真实的细胞间相互作用。这 系统是用户友好的，其自动扫描系统允许进行高速数字和全扫描 在Brightfield或荧光中10-40倍之间。照明系统的性质大大下降 有害效果，例如光漂白和光do污染，因此可以成像更长的标本 时间周期比例如共聚焦显微镜。此外，基于高级机器学习 随附的软件的方法会自动细分并定量组织结构。 Polaris™系统 将纳入我们现有的显微镜核心设施中，以扩大对培训和其他的访问权限 CSU和科罗拉多州北部的研究小组，同时优先考虑主要用户的访问权限。并入 基础核心将维持长期运作，并提供一致的监督和培训。总之， 这种最先进的成像技术对于化学疗法和疫苗的开发至关重要 由MRL，将为CSU的其他多个支持的项目增加价值。