Purchase of an Echo 650 acoustic liquid handler with Access workstation

项目总结 请提供支持，用于购买Echo 650声学液体处理机和Access工作站 为了更换Janus NanoHead，现有的和老化的低容量液体处理机于2008年在 费城维斯塔尔研究所的分子筛选和蛋白质表达设施。这台仪器 升级对基金继续提供最先进的支持，以开发稳健的、 药物发现所需的高通量生化和基于细胞的分析。事实上，Wistar已经做出了一项 致力于学术药物发现，招募了第一位药物化学家约瑟夫·M·萨尔维诺博士，他是 此应用程序的首席调查员。萨尔维诺博士和其他九个持有 总共17项独特的NIH拨款(R01/R35/R61/P01/UM1/U54/DP2)已请求Echo 650访问 开发改进的生化和基于细胞的分析的工作站，以生成更好的数据，从而使 关于新的结构-活性关系的更明智的决策。目前还没有类似的 Wistar调查人员可以接触到的仪器。 Echo 650是一种高精度、低体积、可移动纳升的非接触式液体处理机 利用声波从一个微孔板到另一个微孔板的液体体积。用直接稀释法，可以测定 与传统的串联稀释相比，具有更高精确度的小分子的效力。小型化 这类化验的使用也降低了研究成本。接入工作站是堆叠单元，集成了 ECHO 650，允许高通量筛选和命中挑选所需的自动化板材处理 活性化合物。由于源板之间没有接触，这项技术消除了化合物 结转并保护储存板中现有化合物的完整性。描述的初步数据 在此应用中，我们展示了Echo 650是一项优于我们现有Janus Nanohead的技术。在……里面 此外，我们还表明，我们拥有必要的基础设施，可以将我们的分析小型化到1536孔格式。 该仪器将显著提升我们在检测小型化方面的能力，同时 提高我们的数据质量。 该单元将被安置在分子筛选和蛋白质表达设施中，由Dr。 萨尔维诺，由乔尔·卡塞尔先生管理。萨尔维诺博士和卡塞尔先生加起来都有40多年的经验 有制药行业的工作经验。该设施备受好评，并累计评分 在2018年NIH/NCI癌症中心支持拨款(CCSG)审查中被评为“例外”。我们正在寻求 将我们现有的低容量液体处理技术升级到最先进的非接触式纳升 带有Access工作站的Echo 650中的液体处理技术，以最佳地支持学术 维斯塔尔研究所的药物发现倡议。

PROJECT SUMMARY Support is requested for the purchase of an Echo 650 acoustic liquid handler with Access workstation to replace the Janus NanoHead, the existing and aging low volume liquid handler purchased in 2008 within the Molecular Screening and Protein Expression Facility at The Wistar Institute in Philadelphia. This instrument upgrade is critical for the Facility to continue to provide state-of-the-art support for the development of robust, high-throughput biochemical and cell-based assays required for drug discovery. Indeed, Wistar has made a commitment to academic drug discovery by recruiting its first medicinal chemist, Dr. Joseph M. Salvino, who is the Principal Investigator of this application. Dr. Salvino and nine other major and minor users who hold a combined 17 unique NIH grants (R01/R35/R61/P01/UM1/U54/DP2) have requested the Echo 650 with Access workstation to develop improved biochemical and cell-based assays that generate better data which will enable more informed decision making regarding novel structure-activity relationships. There are currently no similar instruments that are accessible to Wistar investigators. The Echo 650 is a highly accurate, low volume, non-contact liquid handler that moves nanoliter volumes of liquid from one microplate to another using sound waves. Using direct dilution, it can determine the potencies of small molecules with better precision as opposed to traditional serial dilution. The miniaturization of such assays also reduces research costs. The Access workstation is the stacker unit integrated with the Echo 650 which allows for automated plate handling necessary for high-throughput screening and hit picking of active compounds. Because there is no contact between source plates, this technology eliminates compound carry-over and protects the integrity of existing compounds in storage plates. The preliminary data described in this application demonstrate that the Echo 650 is a superior technology to our existing Janus Nanohead. In addition, we also show that we have the necessary infrastructure to miniaturize our assays to 1536-well format. This instrument will significantly upgrade our capabilities regarding assay miniaturization while simultaneously increasing the quality of our data. The unit will be housed in the Molecular Screening and Protein Expression Facility, overseen by Dr. Salvino and managed by Mr. Joel Cassel. Both Dr. Salvino and Mr. Cassel have over 40 years aggregate experience in the pharmaceutical industry. The facility is well regarded and cumulatively ranked with a score of “Exceptional” at the 2018 NIH/NCI Cancer Center support Grant (CCSG) review. We are seeking to upgrade our existing low volume liquid handling technology to a superior state of the art, non-contact nanoliter liquid handling technology in the Echo 650 with Access workstation in order to optimally support the academic drug discovery initiatives of The Wistar Institute.