Community Crystallization Resource for Biological Macromolecules

生物大分子群落结晶资源

• 批准号: 10177813
• 负责人: Edward H Snell
• 金额: $ 24.61万
• 依托单位: HAUPTMAN-WOODWARD MEDICAL RESEARCH INST
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-30 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177813
• 关键词: 3-Dimensional; Accounting; Address; Administrative Supplement; Biological; COVID-19 pandemic; Communities; Crystallization; Crystallography; Databases; Disease; Equipment; Funding; Image; Investigation; Maps; Methods; Outcome; Problem Solving; Process; Proteins; Research; Research Activity; Research Personnel; Resources; Robotics; Sampling; Schedule; Services; Structure; Therapeutic; X-Ray Crystallography; base; data warehouse; design; experimental study; high throughput screening; macromolecule; operation; repository; screening; structural biology; three dimensional structure

1R24GM12135 COMMUNITY CRYSTALLIZATION RESOURCE FOR BIOLOGICAL MACROMOLECULES ADMINISTRATIVE SUPPLEMENT SUPPORT REQUEST PROJECT SUMMARY/ABSTRACT Macromolecular X-ray crystallography is one of the predominant methods used in structural biology, accounting for ~90% of solved structures in the Protein Data Bank, the primary repository and public database for making these three-dimensional structures of biomolecules available to researchers in all fields. To do crystallography, we need crystals. Finding conditions in which a macromolecule will crystallize remains a key challenge in this process, and we have focused on solving this problem at the High-Throughput Crystallization Screening Center. The Crystallization Center is a community resource that provides crystallization experiment set-up and imaging of a uniquely designed set of 1,536 crystallization conditions. Information about macromolecular crystallization is efficiently collected, so that structural investigations for the entire research community can rapidly progress. The Crystallization Center has a robust imaging schedule that facilitates immediate identification of positive crystallization outcomes. The unforeseen circumstances arising due to the extended COVID-19 pandemic have disrupted the operations of the Crystallization Center, as well as the research activities of the wide base of Crystallization Center users. This revision application seeks funds to address this unanticipated need to maintain operations in the Crystallization Center and to facilitate providing continuous uninterrupted services.

1 R24 GM 12135生物大分子群落结晶资源 行政管理支持请求 项目总结/摘要 高分子X射线晶体学是用于结构分析的主要方法之一。 生物学，占蛋白质数据库（主要知识库）中已解决结构的90% 和公共数据库，使这些生物分子的三维结构， 所有领域的研究人员。要做晶体学，我们需要晶体。查找一个 大分子结晶仍然是这一过程中的一个关键挑战，我们已经专注于 解决了高通量结晶筛选中心的这一问题。结晶 中心是一个社区资源，提供结晶实验设置和成像， 独特设计的1，536种结晶条件。关于大分子的信息 有效地收集结晶，以便整个研究的结构研究 社区可以快速发展。结晶中心有一个强大的成像时间表， 有助于立即识别积极的结晶结果。不可预见的 由于COVID-19疫情持续，导致的情况扰乱了 结晶中心，以及结晶的广泛基础的研究活动 中心用户。这一修订申请寻求资金，以解决这一意外的需要， 在结晶中心的操作，并促进提供连续不间断的 服务