Community Crystallization Resource for Biological Macromolecules

生物大分子群落结晶资源

• 批准号: 9370180
• 负责人: Edward H Snell
• 金额: $ 54.19万
• 依托单位: HAUPTMAN-WOODWARD MEDICAL RESEARCH INST
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-30 至 2020-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9370180
• 关键词: Accounting; Back; Biological; Biological Assay; Biology; Chemicals; Collaborations; Communities; Complex; Crystallization; Data; Databases; Deposition; Development; Diagnosis; Disease; Event; Goals; Health; Image; Individual; Institutes; International; Investigation; Knowledge; Laboratories; Lasers; Life; Liquid substance; Medical; Membrane Proteins; Methods; Microscopy; Outcome; Pathogenicity; Physiologic pulse; Process; Protein Structure Initiative; Proteins; Protozoa; Publications; Reagent; Recording of previous events; Records; Reporting; Research Infrastructure; Research Personnel; Resources; Sampling; Scientist; Services; Solubility; Structure; Summary Reports; System; Technology; Therapeutic; Time; Training; X-Ray Crystallography; cost efficient; crystal-8; design; disability; experimental study; high throughput technology; imaging capabilities; imaging system; improved; macromolecule; operation; prevent; research and development; screening; software development; structural biology; structural genomics; submicron; tool; trend

X-ray crystallography is the predominant method used to visualize biological macromolecules at an atomic level of detail. Crystals are mandatory for these structure determinations. The High-Throughput Crystallization Screening (HTS) Laboratory at the Hauptman-Woodward Institute is a community resource that provides crystallization information in a rapid and efficient manner so that structural investigations can rapidly progress. We have developed two sets of 1536 chemical cocktails designed to maximize crystallization potential and chemical solubility information for biological macromolecules for both structural and other scientific applications. In less than ten minutes, liquid-handling systems combine the community-provided sample with chemical cocktails in a single, compact, 1536-well plate. We image the experiments at weekly intervals for six weeks and provide users with immediate access to images. This includes software developed in-house, to view and analyze the results. We employ a state-of-the-art imaging system with a femtosecond pulse laser to detect crystals, including submicron crystals that are too small to see using traditional microscopy. If there are crystals, we are able to detect them. An expert reviews the outcomes for all samples sent to the laboratory to identify crystals and provides this information to the investigators. A graphical summary report provides investigators with relational chemical solubility and crystallization information in readily interpretable formats. The goal of the HTS Laboratory is to provide a rapid turnaround of solubility and crystallization information for community-provided samples to enable rapid scientific process. Our publication records, and associated publications and PDB depositions from structural genomics and the scientific community collaborations support our 15+ year history in the research, development and application of crystallization assays to more than 16,000 samples provided by hundreds of investigators.

X射线晶体学是用于在原子水平上可视化生物大分子的主要方法。 详细程度。晶体对于这些结构测定是强制性的。高放热结晶 筛选（HTS）实验室在豪普特曼伍德沃德研究所是一个社区资源，提供 以快速和有效的方式获得结晶信息，以便结构研究可以快速进行。 我们已经开发了两套1536化学鸡尾酒，旨在最大限度地提高结晶潜力， 生物大分子的化学溶解度信息，用于结构和其他科学 应用.在不到十分钟的时间里，液体处理系统联合收割机将社区提供的样品与 化学鸡尾酒在一个单一的，紧凑的，1536孔板。我们每周对实验进行一次成像， 并为用户提供即时访问图像。这包括内部开发的软件， 查看并分析结果。我们采用了最先进的成像系统与飞秒脉冲激光， 检测晶体，包括使用传统显微镜无法看到的亚微米晶体。如果有 晶体，我们能够检测到它们。一名专家审查所有送往实验室的样本的结果， 识别晶体并将此信息提供给研究人员。图形摘要报告提供 研究人员以易于解释的格式提供相关的化学溶解度和结晶信息。 HTS实验室的目标是提供溶解度和结晶信息的快速周转， 社区提供的样本，以使快速的科学过程。我们的出版记录，以及相关的 来自结构基因组学和科学界合作的出版物和PDB沉积支持 我们在结晶分析的研究、开发和应用方面拥有15年以上的历史， 数百名调查人员提供的样本。