Acquisition of A Microfluidic Chip-Based System for Cluster Sorting and Dispensing

获取基于微流控芯片的集群分选和分配系统

• 批准号: 10177766
• 负责人: Charles KF Chan
• 金额: $ 46.87万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-05-15 至 2022-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177766
• 关键词: Affect; Arterial Fatty Streak; Biology; Blood Vessels; Bone Marrow; COVID-19/ARDS; Cancer Biology; Cell Separation; Cells; Complex; Confocal Microscopy; Core Facility; Disease; Engraftment; Environment; Funding; Goals; Hematopoietic stem cells; Immunology; In Vitro; Institutes; Investigation; Life; Mass Spectrum Analysis; Microfluidic Microchips; Minor; Molecular; Pharmaceutical Preparations; Positioning Attribute; Proteomics; Regenerative Medicine; Request for Proposals; Research; Research Personnel; Severe Acute Respiratory Syndrome; Skin; Sorting - Cell Movement; System; Systems Development; Techniques; Technology; Thymocyte Development; Tissues; Transplantation; Tumor-infiltrating immune cells; United States National Institutes of Health; Universities; Viral Antigens; base; cell type; combat; flu; in vivo; instrument; intravital imaging; mouse model; nerve stem cell; next generation; programs; single-cell RNA sequencing; stem cell biology; tumor; tumor-immune system interactions; wound healing

Project Summary/Abstract This proposal requests funding for a microfluidic chip-based system that will enable cluster sorting and dispensing. This will be the first system made available at a Core facility at Stanford University. The system will fill an established, unmet need for sorting cells in their tissue microenvironment. It will be installed at the FACS Center located at the Institute for Stem Cell Biology and Regenerative Medicine, a Core facility open to all researchers at Stanford University. The major and minor users of the instrument are positioned to apply this instrument along with next generation -omic technologies for molecular and functional characterization of delicate cells and their attached microenvironments with techniques such as in vivo transplantation and in vitro culture, intra vital imaging, single cell RNA sequencing of dissociated clusters, CyTOF analysis, confocal microscopy of living clusters, proteomic analysis using mass spectrometry, to meet the goals of their NIH-funded research programs that include investigations in stem cell biology, vascular biology, cancer biology, and immunology. Stem cell biology • Characterizing the bone marrow niches of hematopoietic stem cells, mechanisms of engraftment of neural stem cells • Analyze how over-the-counter medications can change the immune microenvironment in a mouse model of COVID-19 induced ARDS Vascular biology • Understanding the interplay of different cell types and viral antigens including flu and SARS in complex atherosclerotic lesions Cancer Biology • Understanding tumor micro-environments Immunology • Understanding how resident and infiltrating immune and skin cells affect wound healing • Interrogating the mechanisms behind thymocyte development The system will be placed in a well-established, highly successful core facility open to all researchers at Stanford insures. Over the life of this instrument, it will be used by hundreds of researchers from many tens of labs to support similarly excellent research to better understand and combat disease.

项目概要/摘要 该提案要求为基于微流控芯片的系统提供资金，该系统将实现簇排序和 配药。这将是斯坦福大学核心设施中提供的第一个系统。系统将填充 在组织微环境中对细胞进行分选的既定但未满足的需求。它将安装在 FACS 上 中心位于干细胞生物学和再生医学研究所，这是一个向所有人开放的核心设施 斯坦福大学的研究人员。该工具的主要和次要用户都可以应用此 仪器与下一代组学技术一起用于分子和功能表征 通过体内移植和体外等技术来修复脆弱的细胞及其附着的微环境 培养、活体成像、解离簇的单细胞 RNA 测序、CyTOF 分析、共聚焦 对活体簇进行显微镜检查，使用质谱进行蛋白质组学分析，以实现 NIH 资助的目标 研究项目包括干细胞生物学、血管生物学、癌症生物学等方面的研究 免疫学。 干细胞生物学 • 描述造血干细胞的骨髓生态位、神经细胞植入机制 干细胞 • 分析非处方药如何改变小鼠的免疫微环境 COVID-19 诱导的 ARDS 模型 血管生物学 • 了解不同细胞类型和病毒抗原（包括流感和 SARS）在复杂环境中的相互作用 动脉粥样硬化病变 癌症生物学 • 了解肿瘤微环境 免疫学 • 了解驻留和浸润的免疫细胞和皮肤细胞如何影响伤口愈合 • 探讨胸腺细胞发育背后的机制 该系统将放置在一个完善、非常成功的核心设施中，向斯坦福大学的所有研究人员开放 保证。在该仪器的整个生命周期中，来自数十个实验室的数百名研究人员将使用它 支持类似的优秀研究，以更好地了解和对抗疾病。