Leica STELLARIS 5 Confocal Microscope

Leica STELLARIS 5 共焦显微镜

• 批准号: 10177189
• 负责人: Amber L Alhadeff
• 金额: $ 39.9万
• 依托单位: MONELL CHEMICAL SENSES CENTER
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-05-01 至 2022-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177189
• 关键词: 18 year old; Anosmia; Applications Grants; Basic Science; Brain; COVID-19; Center Core Grants; Chemicals; Data; Equipment; Faculty; Fostering; Functional Imaging; Funding; Health; Histology; Image; Institutes; International; Maintenance; Maps; Microscope; Microscopy; Mosaicism; Nasal cavity; Neuraxis; Organoids; Personal Satisfaction; Productivity; Recording of previous events; Reporting; Research; Research Personnel; Role; Scientific Advances and Accomplishments; Scientist; Services; Smell Perception; Symptoms; System; Taste Perception; Technology; Tongue; Training; United States National Institutes of Health; Visit; data quality; imaging facilities; imaging study; improved; instrument; irritation; novel; nutrition; operation; ranpirnase; recruit; sensory system; undergraduate student

PROJECT SUMMARY/ABSTRACT The Imaging Facility at the Monell Chemical Senses Center is requesting funds to purchase a Leica STELLARIS 5 confocal microscope to support NIH-funded research. The Monell Center is the world’s only independent, nonprofit institute dedicated to interdisciplinary basic research on the chemical senses of taste, smell, gut sensing, and chemical irritation, as well as the relationship between these senses and nutrition, health, and well- being. Recent reports that taste and smell loss are early symptoms of COVID-19 are an example of the increasing importance of this research. Our NIH-funded scientists will use the STELLARIS 5 confocal system to collect imaging data from the nasal cavity, tongue, taste organoids, and brain to generate mosaic compilations for quantitative analysis, to map fine projections within and between sensory systems, and to perform live functional imaging studies. The Monell Center has a strong history of NIH support, and our faculty have unique expertise that attracts national and international collaborators. In this application, we provide strong justification for this equipment request by describing how it will replace our current 18-year-old Leica SP2 confocal system in our Imaging Facility, which will in turn advance scientific discovery by our NIH-funded scientists. The NIH- funded projects we describe in this application represent a broad array of chemosensory research topics in the olfactory, gustatory, and central nervous systems. The requested Leica STELLARIS 5 confocal system will be housed in our Imaging Facility, part of Monell’s Histology and Cellular Localization Core that provides training and services to all scientists, postdoctoral trainees, technical staff, undergraduates, and visiting scientists at the Monell Center. Established in 2011 with support from an NIH P30 Center Core grant, the Histology and Cellular Localization Core has been in operation for 9 years. Since the Histology Core’s inception, the Monell Center has contributed substantial institutional funds to maintain and operate the Core. In this spirit, the Monell Center has agreed to commit a generous amount of funding toward the upgrade, installation, operation, and maintenance of the instrument requested in this application. Overall, obtaining funding for the Leica STELLARIS 5 confocal microscope will revolutionize microscopy in the Imaging Facility at the Monell Center. In addition to its integral role in completion of the current NIH-funded projects, we will use the instrument to develop novel research approaches, foster new grant applications, and recruit new investigators to the Monell Center. Acquisition of this technology will vastly improve research productivity and data quality, expanding the scope of chemosensory research at Monell.

项目总结/摘要 Monell化学传感中心的成像设施正在申请资金购买Leica STELLARIS 5共聚焦显微镜，以支持NIH资助的研究。莫内尔中心是世界上唯一一个独立的， 一个致力于跨学科基础研究的非盈利性机构，研究味觉、嗅觉、肠道等化学感官 感觉和化学刺激，以及这些感觉与营养，健康和健康之间的关系- 存在最近有报道称，味觉和嗅觉丧失是COVID-19的早期症状，这是一个例子， 这项研究的重要性越来越大。我们的NIH资助的科学家将使用STELLARIS 5共聚焦系统， 收集来自鼻腔、舌头、味觉器官和大脑的成像数据，以生成马赛克汇编 用于定量分析，绘制感觉系统内和感觉系统之间的精细投影， 功能成像研究。莫内尔中心有一个强大的历史NIH的支持，我们的教师有独特的 吸引国内外合作者的专业知识。在本申请中，我们提供了强有力的理由， 通过描述它将如何取代我们目前使用了18年的Leica SP2共焦系统， 在我们的成像设备中，这反过来将推动我们NIH资助的科学家的科学发现。国家卫生研究院- 我们在本申请中描述的资助项目代表了化学传感器领域的广泛研究主题。 嗅觉味觉和中枢神经系统要求的Leica STELLARIS 5共焦系统将 位于我们的成像设备中，是Monell组织学和细胞定位核心的一部分， 和服务的所有科学家，博士后学员，技术人员，本科生，并访问科学家在 莫内尔中心。成立于2011年，由NIH P30中心核心资助，组织学和细胞 Localization Core已经运行了9年。自组织学核心成立以来，莫内尔中心 捐助了大量机构资金，以维持和运作核心。本着这种精神，莫内尔中心已 同意投入大量资金用于升级、安装、操作和维护 本申请所要求的文书。总的来说，为Leica STELLARIS 5共焦显微镜获得资金 显微镜将彻底改变显微镜成像设施在莫内尔中心。除了其整体 在完成目前NIH资助的项目中，我们将使用该仪器来开发新的研究 方法，促进新的赠款申请，并招募新的研究人员到莫内尔中心。收购此 技术将大大提高研究效率和数据质量，扩大化学传感器的范围， Monell的研究

项目成果

Alkaline taste sensation through the alkaliphile chloride channel in Drosophila.

• DOI: 10.1038/s42255-023-00765-3
• 发表时间: 2023-03
• 期刊: NATURE METABOLISM
• 影响因子: 20.8
• 作者: Mi, Tingwei;Mack, John O.;Koolmees, Wyatt;Lyon, Quinn;Yochimowitz, Luke;Teng, Zhao-Qian;Jiang, Peihua;Montell, Craig;Zhang, Yali V.
• 通讯作者: Zhang, Yali V.