Leica STELLARIS 5 Confocal Microscope

项目摘要/摘要 莫内尔化学传感中心的成像设备正在申请资金购买徕卡Stellaris 5个共聚焦显微镜，以支持NIH资助的研究。莫内尔中心是世界上唯一的独立的， 非营利机构致力于味觉、嗅觉、肠道化学感官的跨学科基础研究 感觉，和化学刺激，以及这些感觉与营养、健康和健康之间的关系- 是存在的。最近有报道称，味道和嗅觉丧失是新冠肺炎的早期症状，这就是 这项研究的重要性日益增加。我们由NIH资助的科学家将使用Stellaris 5共焦系统来 收集鼻腔、舌头、味觉器官和大脑的影像数据，以生成马赛克汇编 用于定量分析，绘制感官系统内部和之间的精细投射图，并现场执行 功能成像研究。莫内尔中心拥有强大的NIH支持历史，我们的教职员工拥有独特的 吸引国内和国际合作者的专业知识。在本申请中，我们提供了强有力的理由 通过描述它将如何取代我们目前已有18年历史的徕卡SP2共焦系统 在我们的成像设备中，这将反过来促进我们NIH资助的科学家的科学发现。美国国立卫生研究院- 我们在本申请中描述的资助项目代表了化学传感研究的广泛主题 嗅觉、味觉和中枢神经系统。所要求的徕卡Stellaris 5共焦系统将是 安置在我们的成像设备中，这是Monell的组织学和细胞定位核心的一部分，提供培训 并为所有科学家、博士后实习生、技术人员、本科生和访问科学家提供服务 莫内尔中心。在NIH P30中心核心赠款的支持下于2011年成立的组织和细胞 本地化核心已经运行了9年。自组织学核心成立以来，莫内尔中心 为维护和运营核心提供了大量的机构资金。本着这种精神，莫内尔中心 同意投入大量资金用于升级、安装、运营和维护 本申请书中所要求的文书的。总体而言，为徕卡Stellaris 5共焦获得资金 显微镜将使莫内尔中心成像设备中的显微镜发生革命性变化。除了它的整体 在完成目前NIH资助的项目中发挥的作用，我们将使用该仪器来开发新的研究 方法，促进新的拨款申请，并招募新的研究人员到莫内尔中心。收购这一项目 技术将极大地提高研究效率和数据质量，扩大化学传感的范围 在莫内尔大学做研究。

PROJECT SUMMARY/ABSTRACT The Imaging Facility at the Monell Chemical Senses Center is requesting funds to purchase a Leica STELLARIS 5 confocal microscope to support NIH-funded research. The Monell Center is the world’s only independent, nonprofit institute dedicated to interdisciplinary basic research on the chemical senses of taste, smell, gut sensing, and chemical irritation, as well as the relationship between these senses and nutrition, health, and well- being. Recent reports that taste and smell loss are early symptoms of COVID-19 are an example of the increasing importance of this research. Our NIH-funded scientists will use the STELLARIS 5 confocal system to collect imaging data from the nasal cavity, tongue, taste organoids, and brain to generate mosaic compilations for quantitative analysis, to map fine projections within and between sensory systems, and to perform live functional imaging studies. The Monell Center has a strong history of NIH support, and our faculty have unique expertise that attracts national and international collaborators. In this application, we provide strong justification for this equipment request by describing how it will replace our current 18-year-old Leica SP2 confocal system in our Imaging Facility, which will in turn advance scientific discovery by our NIH-funded scientists. The NIH- funded projects we describe in this application represent a broad array of chemosensory research topics in the olfactory, gustatory, and central nervous systems. The requested Leica STELLARIS 5 confocal system will be housed in our Imaging Facility, part of Monell’s Histology and Cellular Localization Core that provides training and services to all scientists, postdoctoral trainees, technical staff, undergraduates, and visiting scientists at the Monell Center. Established in 2011 with support from an NIH P30 Center Core grant, the Histology and Cellular Localization Core has been in operation for 9 years. Since the Histology Core’s inception, the Monell Center has contributed substantial institutional funds to maintain and operate the Core. In this spirit, the Monell Center has agreed to commit a generous amount of funding toward the upgrade, installation, operation, and maintenance of the instrument requested in this application. Overall, obtaining funding for the Leica STELLARIS 5 confocal microscope will revolutionize microscopy in the Imaging Facility at the Monell Center. In addition to its integral role in completion of the current NIH-funded projects, we will use the instrument to develop novel research approaches, foster new grant applications, and recruit new investigators to the Monell Center. Acquisition of this technology will vastly improve research productivity and data quality, expanding the scope of chemosensory research at Monell.