A Modern Flexible Mass Spectrometry Platform for Advancing Proteomics Research

用于推进蛋白质组学研究的现代灵活质谱平台

• 批准号: 10177162
• 负责人: Ryan D. Leib
• 金额: $ 126.89万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-25 至 2022-04-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177162
• 关键词: 11 year old; Acute; Back; Bioinformatics; Biomedical Research; Collaborations; Communicable Diseases; Communities; Complex; Data; Degenerative Disorder; FarGo; Funding; Human Development; Hybrids; Hypersensitivity; Immune response; Infrastructure; Laboratories; Longevity; Mass Spectrum Analysis; Methods; Modernization; Process; Proteomics; Proteomics Shared Resource; Research; Research Personnel; Research Project Grants; Scanning; Technical Expertise; Time; United States National Institutes of Health; Universities; Work; experimental study; flexibility; instrument; instrumentation; mass spectrometer; success

Project Summary/Abstract Stanford researchers are in acute need of a new Orbitrap tribrid mass spectrometer to serve their demanding proteomics needs. This instrument will replace two 11-year-old hybrid Orbitrap instruments which are no longer up to the task. The new instrument will be housed and maintained by Stanford University Mass Spectrometry (SUMS), the campus-wide shared proteomics resource for over 20 years. The previous instruments served well in their time, and our expert staff has been able to coax out cutting edge data long beyond the instruments’ effective lifespans through capable, careful collaboration with hundreds of researchers, but time is now of the essence. As the field of proteomics has evolved from relatively simple, untargeted discovery methods to a experimentally diverse, bioinformatically rich, and robustly integrated suite of research approaches, the existing Orbitrap platforms can no longer meet the demands of today’s NIH-funded biomedical research. The need goes far beyond simple upgrades for greater analytical sensitivity or faster scanning rates; our decade old instruments simply cannot perform the complex, multi-dimensional experiments needed by our researchers on campus today. Numerous NIH-funded projects with pressing bioanalytical questions are being delayed or limited in scope until we are able to provide robust, modern instrument capabilities. Our shared facility maintains an accomplished scientific staff with the necessary technical expertise and drive to support these projects, and our brand-new purpose-built mass spectrometry laboratory provides the ideal physical infrastructure to accommodate new instrumentation seamlessly. Plus, SUMS’ proven record of success as a shared facility for two decades demonstrates the administrative processes are in place to deploy this new mass spectrometer to work on NIH-funded biomedical research projects immediately. Our research community fully backs this proposal as critical to advancing their scientific efforts, which span complex topics in human development, degenerative disease, allergies, infectious disease, and immune response.

项目概要/摘要 斯坦福大学的研究人员迫切需要一种新型 Orbitrap 三联体质谱仪 满足他们苛刻的蛋白质组学需求。该仪器将取代两个 11 年的仪器 混合 Orbitrap 仪器已无法胜任这项任务。新仪器将 由斯坦福大学质谱 (SUMS) 托管和维护， 20多年来全校共享蛋白质组学资源。之前的乐器 在他们的时代服务得很好，我们的专家团队已经能够培养出最先进的技术 通过有能力、细心的人员，可以获取远远超出仪器有效寿命的数据 与数百名研究人员合作，但现在时间至关重要。作为场 蛋白质组学的研究已经从相对简单、无针对性的发现方法发展到 实验多样化、生物信息丰富且稳健集成的研究套件 方法，现有的 Orbitrap 平台已无法满足当今的需求 NIH 资助的生物医学研究。需求远远超出了简单的升级，以获得更大的 分析灵敏度或更快的扫描速率；我们十年前的仪器根本无法 进行我们的研究人员所需的复杂的、多维的实验 今天的校园。许多 NIH 资助的项目都面临着紧迫的生物分析问题 被推迟或范围受到限制，直到我们能够提供强大的现代化仪器 能力。我们的共享设施拥有一支经验丰富的科研人员队伍 必要的技术专业知识和动力来支持这些项目，以及我们全新的 专门建造的质谱实验室提供了理想的物理基础设施 无缝适应新仪器。此外，SUMS 已被证实的成功记录 二十年来的共享设施表明，行政流程是在 部署这种新型质谱仪进行 NIH 资助的生物医学研究的地方 立即项目。我们的研究界完全支持这项提案，认为它对于 推进他们的科学努力，涵盖人类发展的复杂主题， 退行性疾病、过敏、传染病和免疫反应。