A Modern Flexible Mass Spectrometry Platform for Advancing Proteomics Research

项目摘要/摘要 斯坦福大学的研究人员迫切需要一种新的Orbitrap三元质谱仪来 满足他们苛刻的蛋白质组学需求。这台乐器将取代两台11岁的 混合轨道诱捕仪器不再能胜任这项任务。这台新仪器将 由斯坦福大学质谱学(SUM)收藏和维护， 20多年来，校园范围内共享蛋白质组学资源。以前的工具 在他们的时代服务得很好，我们的专家员工已经能够哄骗出最先进的 数据远远超出了仪器的有效寿命，通过有能力、仔细的 与数百名研究人员合作，但现在时间是至关重要的。作为田野 蛋白质组学已经从相对简单、无靶向的发现方法演变成 实验多样化、丰富的生物信息和强有力的集成研究套件 方法，现有的Orbitrap平台已不能满足当今的需求 美国国立卫生研究院资助的生物医学研究。需要的远不止简单的升级，而是更大的 分析灵敏度或更快的扫描速度；我们十年前的仪器根本不能 执行我们的研究人员所需的复杂、多维实验 今天的校园。许多由NIH资助的项目面临紧迫的生物分析问题 延迟或范围受限，直到我们能够提供强大的、现代化的仪器 能力。我们的共享设施拥有一支经验丰富的科学工作人员 支持这些项目所需的技术专长和动力，以及我们全新的 专门建造的质谱学实验室提供了理想的物理基础设施 无缝适应新的仪器设备。此外，萨姆斯公司已被证明的成功记录 作为一个共享设施20年来，证明了管理流程是在 部署这种新型质谱仪进行美国国立卫生研究院资助的生物医学研究的地点 立即进行项目。我们的研究界完全支持这一建议，认为这对 推进他们跨越人类发展复杂主题的科学努力， 退行性疾病、过敏、传染病和免疫反应。

Project Summary/Abstract Stanford researchers are in acute need of a new Orbitrap tribrid mass spectrometer to serve their demanding proteomics needs. This instrument will replace two 11-year-old hybrid Orbitrap instruments which are no longer up to the task. The new instrument will be housed and maintained by Stanford University Mass Spectrometry (SUMS), the campus-wide shared proteomics resource for over 20 years. The previous instruments served well in their time, and our expert staff has been able to coax out cutting edge data long beyond the instruments’ effective lifespans through capable, careful collaboration with hundreds of researchers, but time is now of the essence. As the field of proteomics has evolved from relatively simple, untargeted discovery methods to a experimentally diverse, bioinformatically rich, and robustly integrated suite of research approaches, the existing Orbitrap platforms can no longer meet the demands of today’s NIH-funded biomedical research. The need goes far beyond simple upgrades for greater analytical sensitivity or faster scanning rates; our decade old instruments simply cannot perform the complex, multi-dimensional experiments needed by our researchers on campus today. Numerous NIH-funded projects with pressing bioanalytical questions are being delayed or limited in scope until we are able to provide robust, modern instrument capabilities. Our shared facility maintains an accomplished scientific staff with the necessary technical expertise and drive to support these projects, and our brand-new purpose-built mass spectrometry laboratory provides the ideal physical infrastructure to accommodate new instrumentation seamlessly. Plus, SUMS’ proven record of success as a shared facility for two decades demonstrates the administrative processes are in place to deploy this new mass spectrometer to work on NIH-funded biomedical research projects immediately. Our research community fully backs this proposal as critical to advancing their scientific efforts, which span complex topics in human development, degenerative disease, allergies, infectious disease, and immune response.