A Modern Flexible Mass Spectrometry Platform for Advancing Proteomics Research

项目总结/摘要 斯坦福大学的研究人员迫切需要一种新的Orbitrap三重质谱仪， 满足他们苛刻的蛋白质组学需求。这台仪器将取代两台11岁的 已经不能胜任任务的混合轨道阱仪器。新工具将 由斯坦福大学质谱仪（SUMS）， 20多年来一直是全校共享的蛋白质组学资源。以前的文书 在他们的时间很好地服务，我们的专家工作人员已经能够哄出尖端 数据远远超出仪器的有效寿命，通过有能力，仔细 与数百名研究人员的合作，但现在时间是至关重要的。随着该领域 蛋白质组学已经从相对简单的，无针对性的发现方法发展到 实验多样，生物信息丰富，强大的综合研究套件 现有的Orbitrap平台已经不能满足当今的需求， NIH资助的生物医学研究。需要远远超出简单的升级， 分析灵敏度或更快的扫描速度;我们十年前的仪器根本无法 执行我们的研究人员所需的复杂，多维的实验， 校园今天许多NIH资助的具有紧迫生物分析问题的项目， 在我们能够提供强大的现代仪器之前， 能力的我们的共享设施保持了一个有成就的科学工作人员与 必要的技术专长和动力来支持这些项目，以及我们全新的 专门建造的质谱实验室提供理想的物理基础设施， 无缝地适应新仪器。此外，SUMS的成功记录 作为一个共享设施的二十年表明，行政程序是在 在NIH资助的生物医学研究中部署这种新型质谱仪的地方 项目立即我们的研究界完全支持这一提议，认为这对 推进他们的科学努力，这些努力涉及人类发展的复杂主题， 退行性疾病、过敏症、传染病和免疫反应。

Project Summary/Abstract Stanford researchers are in acute need of a new Orbitrap tribrid mass spectrometer to serve their demanding proteomics needs. This instrument will replace two 11-year-old hybrid Orbitrap instruments which are no longer up to the task. The new instrument will be housed and maintained by Stanford University Mass Spectrometry (SUMS), the campus-wide shared proteomics resource for over 20 years. The previous instruments served well in their time, and our expert staff has been able to coax out cutting edge data long beyond the instruments’ effective lifespans through capable, careful collaboration with hundreds of researchers, but time is now of the essence. As the field of proteomics has evolved from relatively simple, untargeted discovery methods to a experimentally diverse, bioinformatically rich, and robustly integrated suite of research approaches, the existing Orbitrap platforms can no longer meet the demands of today’s NIH-funded biomedical research. The need goes far beyond simple upgrades for greater analytical sensitivity or faster scanning rates; our decade old instruments simply cannot perform the complex, multi-dimensional experiments needed by our researchers on campus today. Numerous NIH-funded projects with pressing bioanalytical questions are being delayed or limited in scope until we are able to provide robust, modern instrument capabilities. Our shared facility maintains an accomplished scientific staff with the necessary technical expertise and drive to support these projects, and our brand-new purpose-built mass spectrometry laboratory provides the ideal physical infrastructure to accommodate new instrumentation seamlessly. Plus, SUMS’ proven record of success as a shared facility for two decades demonstrates the administrative processes are in place to deploy this new mass spectrometer to work on NIH-funded biomedical research projects immediately. Our research community fully backs this proposal as critical to advancing their scientific efforts, which span complex topics in human development, degenerative disease, allergies, infectious disease, and immune response.