Microscale Thermophoresis

微尺度热泳

• 批准号: 10177014
• 负责人: Jared Auclair
• 金额: $ 36.84万
• 依托单位: NORTHEASTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-25 至 2022-04-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177014
• 关键词: Affinity; Antibiotic Resistance; Area; Binding; Boston; Charge; Communicable Diseases; Detection; Development; Drug Design; Fluorescence; Generations; Hydration status; Immobilization; Inflammation; Label; Ligand Binding; Malignant Neoplasms; Measures; Molecular; Monitor; Research Personnel; Sampling; Temperature; Virus Replication; addiction; base; flexibility; instrument; migration; nervous system disorder

Project Summary We propose to acquire a NanoTemper Monolith NT-Auto, with detection of both fluorescently-labeled molecules and of intrinsic fluorescence. This instrument provides maximum flexibility for investigators and will be the only instrument of its kind in the Boston area. The Monolith instruments measure microscale thermophoresis, which is based on the generation of a precise temperature gradient while monitoring changes in sample migration along this gradient, as detected by sample fluorescence to determine binding. The changes in migration of molecules along the temperature gradient reflect changes in molecular hydration shell, charge, or size; as one or all of these parameters change upon ligand binding, differences in the bound and unbound states can be determined. The requested instrument allows determination of binding affinities, oligomerization, and other parameters without immobilization. The instrument will be used to advance numerous projects related to cancer, antibiotic resistance, infectious disease, viral replication, inflammation, and neurological disorders including addiction. A second major focus is on drug design, development, and delivery.

项目摘要 我们建议获取纳米机nt-nt-auto，并检测到两个荧光标记 分子和内在荧光。该工具为调查人员提供了最大的灵活性，将 成为波士顿地区唯一同类工具。 Monolith仪器测量了微观 热疗法基于确切的温度​​梯度的产生，同时监测变化 在沿该梯度的样品迁移中，如样品荧光检测到的结合。这 分子沿温度梯度迁移的变化反映了分子水合壳的变化， 充电或大小；随着一个或全部这些参数在配体结合时发生变化，绑定的差异和 可以确定未结合状态。请求的仪器允许确定约束亲和力， 寡聚和其他参数而无需固定。该乐器将用于推进 许多与癌症，抗生素抗性，传染病，病毒复制，炎症有关的项目 和神经系统疾病，包括成瘾。第二个主要重点是药物设计，开发和 送货。