Microscale Thermophoresis

微尺度热泳

• 批准号: 10177014
• 负责人: Jared Auclair
• 金额: $ 36.84万
• 依托单位: NORTHEASTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-25 至 2022-04-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177014
• 关键词: Affinity; Antibiotic Resistance; Area; Binding; Boston; Charge; Communicable Diseases; Detection; Development; Drug Design; Fluorescence; Generations; Hydration status; Immobilization; Inflammation; Label; Ligand Binding; Malignant Neoplasms; Measures; Molecular; Monitor; Research Personnel; Sampling; Temperature; Virus Replication; addiction; base; flexibility; instrument; migration; nervous system disorder

Project Summary We propose to acquire a NanoTemper Monolith NT-Auto, with detection of both fluorescently-labeled molecules and of intrinsic fluorescence. This instrument provides maximum flexibility for investigators and will be the only instrument of its kind in the Boston area. The Monolith instruments measure microscale thermophoresis, which is based on the generation of a precise temperature gradient while monitoring changes in sample migration along this gradient, as detected by sample fluorescence to determine binding. The changes in migration of molecules along the temperature gradient reflect changes in molecular hydration shell, charge, or size; as one or all of these parameters change upon ligand binding, differences in the bound and unbound states can be determined. The requested instrument allows determination of binding affinities, oligomerization, and other parameters without immobilization. The instrument will be used to advance numerous projects related to cancer, antibiotic resistance, infectious disease, viral replication, inflammation, and neurological disorders including addiction. A second major focus is on drug design, development, and delivery.

项目总结