X-ray Diffraction System

X射线衍射系统

• 批准号: 10177452
• 负责人: CHRISTOPHER P. HILL
• 金额: $ 59.69万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2022-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177452
• 关键词: Aging; Anodes; Biochemistry; Biological Process; Cell Proliferation; Cell division; Cells; Chemicals; Chemistry; Core Facility; Crystallization; Data; Data Set; Ensure; Focus Groups; Funding; Goals; Hybrids; Image; Lighting; Maintenance; Mediating; Mission; Modernization; Molecular; Nucleic Acids; Optics; Performance; Preparation; Recovery; Research; Research Personnel; Resolution; Resources; Roentgen Rays; Sampling; Scientist; Source; Structure; Sulfur; System; Talents; Transcriptional Regulation; Transition Elements; United States National Institutes of Health; Universities; Update; Utah; Vendor; Virus Replication; X ray diffraction analysis; X-Ray Crystallography; experience; instrumentation; novel; oxidation; photon-counting detector; programs; recruit; self assembly; small molecule; synergism; therapeutic development

Project Summary/Abstract The University of Utah Departments of Biochemistry and Chemistry are proposing to acquire a new Rigaku XtalLab Synergy-DW single crystal X-ray diffractometer (scXRD) equipped with a high brilliance, dual wavelength (Cu and Mo Kα) micro-focus rotating anode X-ray source; hybrid photon counting detector with an ultra-wide dynamic range; X-ray beam optics optimized for dual wavelength with fully adjustable divergence slit controls; 4-circle Kappa goniometer; and a modern electronically controlled cabinet with sample lighting, high-resolution crystal video imaging, and ergonomic accessibility to crystal mounting and recovery. The requested system will replace aging instrumentation in both of these departments that are routinely utilized by a large number of NIH- funded investigators, but where the entire system or key components no longer have vendor support and are experiencing increasing maintenance challenges. Further, the shared X-ray instrumentation upgrade needs within these two departments has provided the impetus for developing a new University of Utah X-ray Crystallography Core Facility, where the proposed scXRD system would serve as the centerpiece. Thus, the overall goal of this proposal is to update and replace aging scXRD instrumentation, while simultaneously ensuring continued NIH funded investigator accessibility to in-house state-of-the-art X-ray resources, as well as reducing redundancy by consolidating instrumentation and core facility resources. In particular, the proposed dual Cu and Mo Kα wavelength rotating anode X-ray source will afford structure/function analyses for diverse chemical and biological processes including novel chemical compounds for therapeutic development, transition- metal mediated small molecule activation, supra molecular chemistry via self-assembly, cellular oxidation of nucleic acids, transcriptional regulation, cell division and proliferation, and viral replication. Pairing of this X-ray source with vendor specific optics assembly and extremely sensitive hybrid photon counting detector with small pixel size will greatly enhance research capabilities in multiple profound ways. Specifically, for groups focused on characterization of small molecules, the proposed scXRD features will afford analysis of small weakly diffracting crystals and determination of absolute stereochemical configuration, capabilities not currently available. Analogously, for characterization of macromolecular systems, features of this high-performance instrumentation, including the capacity to vary beam divergence, will afford acquisition of high-quality data sets from small crystals, including those with large unit cells. In addition, the ability of the requested system to collect sulfur-SAD data will facilitate determination of novel structures without the preparation of heavy atom derivatives or the incorporation of selenomethione, which can be problematic for many especially valuable targets. In short, acquisition of the requested scXRD system will support and enhance research capabilities for a considerable number of existing NIH-funded programs, and will be critical to the broader University of Utah mission of retaining, recruiting and supporting talented scientists in NIH relevant, biomedically related fields.

项目摘要/摘要 犹他大学生物化学和化学系提议收购一家新的Rigaku XtalLab Synergy-DW高亮度双波长单晶X射线衍射仪 (Cu，Mo，K，α)微聚焦旋转阳极X射线源超宽混合光子计数探测器 动态范围；X射线光束光学优化的双波长与完全可调的发散狭缝控制； 4圈Kappa测角仪；现代电控柜，带样品照明，高分辨率 水晶视频成像，以及符合人体工程学的水晶安装和回收。所请求的系统将 更换这两个部门中大量NIH经常使用的老化仪器- 资助调查人员，但整个系统或关键组件不再有供应商支持，并且 面临着越来越多的维护挑战。此外，共享X射线仪器需要升级 在这两个系内提供了发展一个新的犹他大学X射线的动力 结晶学核心设施，拟议的SCX射线系统将作为中心。因此， 这项提议的总体目标是更新和更换老化的scx射线衍射仪，同时 确保NIH资助的调查员继续获得内部最先进的X光资源以及 通过整合仪器设备和核心设施资源来减少冗余。特别是，拟议的 双铜钼Kα波长旋转阳极X射线源将为不同类型的 化学和生物过程，包括用于治疗开发、过渡和 金属介导的小分子活化，自组装超分子化学，细胞氧化 核酸、转录调控、细胞分裂和增殖以及病毒复制。这张X射线的配对 光源具有供应商专用光学组件和极灵敏的混合光子计数探测器 像素大小将以多种深刻的方式极大地增强研究能力。具体地说，对于专注于 在小分子的表征方面，所提出的scx射线衍射谱特征将为小分子的弱分析提供依据。 衍射晶体和绝对立体化学构型的测定 可用。同样，对于表征大分子体系，这种高性能的特点 仪器设备，包括改变光束发散角的能力，将提供高质量的数据集 来自小晶体，包括那些具有大单胞的晶体。此外，请求的系统收集 硫SAD数据将有助于在不制备重原子衍生物的情况下确定新的结构 或者硒蛋氨酸的掺入，这对许多特别有价值的目标来说可能是有问题的。总之, 购买所需的SCXD系统将在相当大程度上支持和加强研究能力 NIH现有资助项目的数量，将对犹他大学更广泛的任务至关重要 留住、招募和支持NIH相关、生物医学相关领域的有才华的科学家。