ShEEP Request for KEYENCE BZ-X800 All-in-One Fluorescence Microscope

项目总结/摘要 Keyence BZ-X00是一款多功能、多用途的细胞和组织显微镜平台。而纳入 6个物镜，放大倍率从2X到100X。它可用于成像与白色光， 整个组织切片和培养细胞的组织学检查，以及使用荧光成像滤光片 整个色谱。本提案中的BZ-X800配置包括一个集成加热室 使用CO2进行扩展的活细胞成像，由包含的自动焦平面校正软件提供便利。它 还包括一个可选的网格系统，用于结构化照明，可以在光线照射到 样品，以消除焦点外的光，并提供图像与共焦显微镜的分辨率。任择 数据分析软件包包括自动拼接大图像，混合细胞计数和3D， 细胞和组织的重建。它从根本上被设计为一个带外壳的独立系统 它取代了对暗室的需求，并通过软件控制将用户错误的可能性降至最低 导致硬件故障。 ·Lee：Current VA Merit，5I01 BX 003265 - 05，检查MIF-CD74信号传导在保护中的机制 通过激活特定的细胞存活、抗氧化剂和DNA修复分子来对抗肺气肿， 衰老蛋白p16的抑制。 ·李：目前的国防部赠款，PR150809，正在测试MIF增强对香烟烟雾相关的 COPD及其后续并发症，肺炎链球菌肺炎 ·Spurney：待VA Merit更新：I01 BX 002984 - 05检查了有助于 糖尿病肾病肾小球足细胞的损伤 ·Spurney：国防部补助金（PR180545）和NIH培训补助金（T32 DK007731），是一个共同的 R01研究员（R01-DK098135）。 ·米勒：目前VA Merit，I01 BX 001729，正在研究慢性炎症的分子机制。 加速血管疾病研究在培养的平滑肌细胞和小鼠模型中进行。 颈动脉损伤 ·米勒：待定VA Merit，I01 BX 005308，是一项IND前启用研究，旨在检查合成RNA的安全性 用于治疗过度增殖性血管疾病的配体。 ·米勒：I01 BX 005088是一项为期四年的Merit，它将鉴定选择性结合炎症的RNA适体。 内皮细胞和另一种适体以抑制平滑肌细胞生长。研究在培养的 细胞和血管疾病的鼠模型。 ·米勒：I01 BX 005450是一项为期两年的Merit，旨在确定细胞外 组蛋白通过先前描述的合成RNA配体将保护免于急性炎症的发展。 呼吸窘迫综合征（ARDS）。这些研究在仓鼠模型中进行， 新冠肺炎。 ·Pisetsky：VA Merit，5I01BX 003772 - 02，SLE中自身抗原的细胞来源 ·McMahon：VA Merit，VA BX-003478 - 01。SNO转运调节红细胞的内皮粘附。目标是 以确定红细胞输出和内皮细胞输入SNO的机制，形成信号传导， 通路 Tighe：该实验室对空气污染改变肺结构和功能的机制感兴趣。 ·默多克：我们的研究涉及慢性炎症对合并症和终末器官疾病的影响， 包括气道疾病和药物滥用导致的炎症。

Project Summary / Abstract The Keyence BZ-X00 is a multi-functional and versatile microcopy platform for cells and tissues. It incorporates 6 objective lenses with magnification power from 2X up to 100X. It can be used to image with white light for histological examination of whole tissue slices and cells in culture, and by using filters for fluorescence imaging across the color spectrum. The BZ-X800 configuration in this proposal includes an integrated heating chamber with CO2 for extended live-cell imaging, facilitated by the included automatic focal plane correction software. It also incorporates an optional grid system for structured illumination that can be inserted before the light hits the sample to remove out of focus light and provide images with the resolution of confocal microscopes. Optional data analysis packages are included for automated stitching of large images, hybrid cell counting and 3D- reconstructions of cells and tissues. It is designed from the ground up as a stand-alone system with an enclosure that replaces the need for a dark room and with software controls that minimize the possibility of user errors leading to hardware malfunctions. • Lee: Current VA Merit, 5I01 BX003265-05, examining mechanisms of MIF-CD74 signaling in protecting against emphysema through activation of specific cell survival, antioxidant, and DNA repair molecules and the suppression of senescence protein p16. • Lee: Current DoD Grant, PR150809, is testing MIF augmentation against cigarette smoke exposure-related COPD and its subsequent complication, Streptococcus pneumoniae pneumonia • Spurney: Pending VA Merit Renewal: I01 BX002984-05 examines the molecular mechanisms that contribute to injury of glomerular podocytes in diabetic kidney disease • Spurney: Department of Defense grant (PR180545) and an NIH training grant (T32 DK007731) and is a co- investigator on an active R01 grant (R01-DK098135). • Miller: Current VA Merit, I01 BX001729, is examining molecular mechanisms by which chronic inflammation accelerates vascular disease. Studies are performed in cultured smooth muscle cells and a murine model of carotid injury. • Miller: Pending VA Merit, I01 BX005308, is a pre-IND enabling study to examine the safety of Synthetic RNA ligands for the treatment of hyperproliferative vascular disease. • Miller: I01 BX005088 is a four-year Merit that will identify RNA aptamers that selectively bind to inflamed endothelial cells and another aptamer to inhibit smooth muscle cell growth. Studies are performed in cultured cells and murine models of vascular disease. • Miller: I01 BX005450 is a two-year Merit that proposes to determine whether inactivation of extracellular histones by a previously described synthetic RNA ligand will protect from the development of acute respiratory distress syndrome (ARDS) in COVID-19. These studies are performed in hamster models of COVID-19. • Pisetsky: VA Merit, 5I01BX003772-02, Cellular Sources of Self Antigen in SLE • McMahon: VA Merit, VA BX-003478-01. SNO transport regulates endothelial adhesion of RBCs. The goal is to determine the mechanism by which RBCs export and endothelial cells import SNOs, forming a signaling pathway. • Tighe: The laboratory is interested in mechanisms by which air pollution alters lung structure and function. • Murdoch: Our studies relate to the impact of chronic inflammation on comorbidities and end organ disease, including airway disease and substance abuse-driven inflammation.