Interaction of ambient ammonia and endotoxins with airborne and human nasal microbiome diversity in communities near large-scale poultry feeding operations

大型家禽饲养场附近社区环境氨和内毒素与空气和人类鼻腔微生物多样性的相互作用

• 批准号: 10178028
• 负责人: Margaret L. Phillips
• 金额: $ 18.13万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-03 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10178028
• 关键词: Affect; Agriculture; Air; Ammonia; Anaerobic Bacteria; Atmosphere; Bacteria; Biological; Cherokee Indian; Communities; Consumption; Data; Domestic Fowls; Endotoxins; Environment; Environmental Exposure; Environmental Health; Environmental Policy; Environmental Pollution; Exposure to; Eye; Farming environment; Future; Gases; Geography; Goals; Gram-Negative Bacteria; Health; Human; Investigation; Lead; Link; Lipopolysaccharides; Lung; Lung diseases; Manure; Measurement; Membrane; Metabolism; Metagenomics; Methods; Molecular; Mucous Membrane; Mucous body substance; Nasal cavity; Nasopharynx; Nose; Oklahoma; Particle Size; Pattern; Play; Policy Making; Production; Public Health; Respiratory System; Role; Statutes and Laws; Surveys; Taxonomy; Time; Upper respiratory tract; agricultural community; airway epithelium; airway inflammation; base; cohort; community based participatory research; effectiveness evaluation; environmental change; evidence base; experience; feeding; longitudinal design; microbial; microbiome; microbiota; multiple omics; nasal microbiome; novel; operation; particle; residence; respiratory; respiratory health; tribal member

PROJECT SUMMARY/ABSTRACT Due to the increase in poultry consumption nationally, the construction of mega-sized poultry feeding operations (PFO) in northeast Oklahoma is proceeding rapidly. The Oklahoma State Board of Agriculture approved a setback rule that imposes geographic restrictions on PFO. This rule, which is much less stringent than the previously proposed setback legislation, is not based on empirical exposure data. Since the rule went into effect on September 15, 2019, a substantial number of new PFO will be built, or existing PFO expanded, within a couple of years. Thus, an investigation of environmental health concerns in the affected community is urgently needed. The wave of new PFO construction that will ensue provides a time-sensitive opportunity for a baseline assessment of environmental exposures and microbiomes before the new PFO are stocked, followed by longitudinal post-assessments at critical times in the poultry raising cycle. PFO emit contaminants into the ambient atmosphere that can change the environmental and human nasal microbiome. The most common airborne contaminants emitted from poultry environments are ammonia and endotoxin, which is generated by production operations, manure storage, and land spreading of poultry litter. Ammonia is an odorous gas that results from the anaerobic decomposition of manure and carcasses. Numerous studies have found evidence that exposure to ammonia can irritate the eyes, nose, and mucous membranes in the upper respiratory tract, causing respiratory diseases. Similarly, endotoxin, the lipopolysaccharide part of the outer membrane of a gram-negative bacteria, is an odorless component that contributes to airway inflammation and plays a significant role in the adverse respiratory effects experienced in agricultural communities. Yet, the link between airborne contaminants from PFO and the related biological impact using a nasal microbiome have not been investigated in community-based participatory research. In this study, we will investigate the factors and mechanisms contributing to contamination levels to modulate the potential impact of PFO on residents' respiratory systems. Our central hypothesis is that living closer to a new PFO will be associated with a higher level of environmental exposure, which in turn will alter nasal microbiome patterns. The two goals of this time- sensitive R21 are 1) to assess levels of ammonia and size distribution of endotoxin particles in the air as a function of the resident's proximity to PFO and 2) to assess the interaction between the taxonomic community composition of the microbiota in the contaminated air and in the human nasal microbiome. The results of this study will enhance the current understanding of the mechanisms by which elevated levels of airborne contaminants from PFO alter the composition of the microbiota in air, eventually impacting respiratory health through changes in the composition and function of the nasal microbiome. The environmental metagenomics data longitudinally collected from this unique cohort will provide support for future studies using novel multi- omics approaches.

项目摘要/摘要 由于全国家禽消费的增加，大型家禽喂养的建造 俄克拉荷马州东北部的运营（PFO）正在迅速进行。俄克拉荷马州农业委员会 批准了对PFO施加地理限制的挫折规则。这个规则，这不那么严格 比以前提出的挫折立法不是基于经验暴露数据。由于规则走了 生效为2019年9月15日生效，将建造大量新的PFO或现有的PFO扩展， 几年之内。因此，对受影响社区的环境健康问题的调查是 迫切需要。随之而来的新PFO结构浪潮为一个时间敏感的机会 在库存新的PFO之前，对环境暴露和微生物组的基线评估，遵循 通过在禽类升高周期的关键时期进行纵向后评估。 PFO散发污染物 可以改变环境和人类鼻微生物组的环境气氛。最常见的 从家禽环境中发出的空气传播污染物是氨和内毒素，这是由 禽窝的生产操作，肥料存储和土地蔓延。氨是一种有可能的气体 肥料和尸体的厌氧分解结果。许多研究发现了证据 暴露于氨可能会刺激上呼吸道中的眼睛，鼻子和粘膜， 引起呼吸系统疾病。同样，内毒素，外膜的脂多糖部分 革兰氏阴性细菌是一种无味的成分，会导致气道炎症并发挥作用 在农业社区中遇到的不良呼吸影响中的重要作用。但是， 来自PFO的空气传播污染物和使用鼻微生物组的相关生物学影响尚未 在基于社区的参与性研究中进行了调查。在这项研究中，我们将研究因素和 导致污染水平的机制调节PFO对居民的潜在影响 呼吸系统。我们的中心假设是，与新的PFO更近的生活将与更高 环境暴露水平又会改变鼻微生物组模式。这个时代的两个目标 - 敏感的R21是1）评估空气中内毒素颗粒的氨和大小分布的水平 居民靠近PFO的功能和2）评估分类学界的相互作用 污染空气和人鼻微生物组中微生物群的组成。结果的结果 研究将增强对机制升高机制的当前理解 PFO的污染物改变了空气中微生物群的组成，最终影响呼吸健康 通过变化鼻微生物组的组成和功能。环境宏基因组学 从这个独特的队列中纵向收集的数据将为将来的研究提供支持的支持 OMICS的方法。