Molecular Biology and Cytometry Research Shared Resource

分子生物学与细胞学研究共享资源-摘要 分子生物学和细胞学研究共享资源(MBCR SR)是一个机构管理的 中央核心设施，协助调查人员进行所有方面的研究，包括初步项目咨询， 协助实验设计、方案和仪器使用方面的培训、样品制备、数据 收集并协助分析和解释结果。在詹姆斯·托马塞克的指导下， 博士，OUHSC负责研究的副总裁，MBCR SR为SCC提供四项重要的核心服务 成员和其他调查人员：1)DNA测序和基因组学核心为调查人员提供 获得高保真的新一代核酸测序数据；2)质谱学和 蛋白质组学核心为生物分子鉴定提供蛋白质组学支持，包括复杂蛋白质组学 分析，翻译后修饰鉴定，高分辨率分子量测定，大型 生物分子质量测量，LC-UV-MS分析(定性或定量)，蛋白质鉴定 蛋白水解酶(凝胶内或溶液中)，纳米LC/MS/MS和数据库搜索，以及多肽测序；3) 流式细胞术和成像核心为亚细胞研究提供流式细胞术和显微镜支持 蛋白质表达、细胞周期和细胞信号；以及4)癌症功能基因组核心提供切割- 用于高通量活细胞癌细胞功能表征的EDGE技术和服务，包括 反向蛋白质组学阵列建立、绝对基因定量和拷贝数测定、核 酸和蛋白质电泳和成像，用于平板高通量分析的多模式筛选，高通量 吞吐量、细胞内亚细胞定位蛋白的高含量筛选、活细胞的代谢分析和 肿瘤细胞克隆形成试验集落的定量。 MBCR SR总共占用6,409 SF。斯坦顿L的1、3和4层的实验室空间。 位于OUHSC校园的青年生物医学研究中心(BRC)设施。这个中心位置提供了 27个基于BRC的SCC成员可以立即访问这一重要的基础设施。额外的五个SCC 成员们位于毗邻的生物医学科学大楼(BMSB)，该大楼有专门的内部 通往BRC的人行道。2016年，MBCR SR用户总数中有22%是经过同行审查的SCC成员 支持。该设施还为12名SCC成员提供服务，没有经过同行审查的支持。2016年，SCC成员 占这一中央机构设施总用户的32%，这是迄今为止 OUHSC的部门或疾病现场小组，强调该设施的高利用率和对 在SCC推进研究。SCC发起的激励计划涵盖所有收取的费用的20% SCC成员为MBCR所有区域提供的服务。

MOLECULAR BIOLOGY AND CYTOMETRY RESEARCH SHARED RESOURCE – ABSTRACT The Molecular Biology and Cytometry Research Shared Resource (MBCR SR) is an institutionally managed central core facility that assists investigators in all aspects of research, including initial project consultation, assistance with experimental design, training in protocols and instrumentation usage, sample preparation, data collection and assistance with analysis and interpretation of results. Under the direction of James Tomasek, PhD, the OUHSC Vice President for Research, the MBCR SR provides four important Core services to SCC members and other investigators: 1) the DNA Sequencing and Genomics Core provides investigators with access to high-fidelity, next-generation nucleic acid sequencing data; 2) the Mass Spectrometry and Proteomics Core provides proteomics support for biomolecule identification, including complex proteomics analysis, post-translational modification identification, high-resolution molecular weight determination, large biomolecular mass measurements, LC-UV-MS analysis (qualitative or quantitative), protein identification by proteolytic digest (in-gel or in solution), nano LC/MS/MS and database search, and peptide sequencing; 3) the Flow Cytometry and Imaging Core provides flow cytometry and microscopy support for subcellular studies on protein expression, cell cycle and cell signaling; and 4) the Cancer Functional Genomics Core provides cutting- edge technology and services for high-throughput live cell functional characterization of cancer cells, including reverse proteomics array establishment, absolute gene quantification and copy number determination, nucleic acid and protein electrophoresis and imaging, multimodal screening for high-throughput assay on plates, high- throughput, high-content screening of subcellular localized proteins in cells, metabolic analysis of live cells and quantification of clonogenic assay colonies of cancer cells. The MBCR SR occupies a total of 6,409 sf. of laboratory space on the 1st, 3rd and 4th floors in the Stanton L. Young Biomedical Research Center (BRC) facility on the OUHSC campus. This central location provides the 27 BRC-based SCC members with immediate access to this important infrastructure. An additional five SCC members are located in the adjacent Biomedical Sciences Building (BMSB), which has a dedicated internal walkway to BRC. In 2016, 22% of the total number of MBCR SR users were SCC members with peer-reviewed support. The facility also served 12 SCC members without peer-reviewed support. In 2016, SCC members constituted 32% of the total users of this centralized institutional facility, which is by far the largest usage by a department or disease-site group at OUHSC, highlighting the high utilization and impact that the facility has on advancing research at the SCC. An incentive program initiated by the SCC covers 20% of all charged fees for SCC members for services provided by all areas of the MBCR.