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Control of lymphocyte homeostasis by iCD8a cells and osteopontin

iCD8a 细胞和骨桥蛋白对淋巴细胞稳态的控制

基本信息

批准号：
10178005
负责人：
Danyvid Olivares-Villagomez
金额：
$ 35.55万
依托单位：
VANDERBILT UNIVERSITY MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-07-05 至 2022-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10178005
关键词：
Adoptive Transfer Anti-CD40 Antibody Therapy Area B-Cell Antigen Receptor Biological Biology Cells Citrobacter rodentium Data Development Disease Disease model Enhancers Environment Epithelial Foundations Gene Expression Profile Grant Health Homeostasis Human Immune Immune response Immunodeficient Mouse Immunologics Infection Inflammation Inflammatory Inflammatory Bowel Diseases Inflammatory Response Interleukin-12 Intestinal Diseases Intestines Knowledge Ligands Lymphocyte Lymphocyte Biology Lymphocyte Subset Lymphoid Lymphoid Cell Maintenance Modeling Mouse Strains Mucosal Immune Responses Mucous Membrane Mus Osteogenesis Pathogenicity Phenotype Population Preventive Process Production Publications Research Proposals Role Source Stimulus T-Lymphocyte Therapeutic Tissues Wild Type Mouse chemokine cytokine effector T cell inflammatory disease of the intestine intestinal epithelium intraepithelial microorganism novel osteopontin prophylactic tissue regeneration

项目摘要

SUMMARY In this application we propose to investigate the biological features of innate CD8α cells (or iCD8α cells), a novel lymphoid population present in the intestinal epithelium discovered by our group. A hallmark of this population is the expression of CD8αα, lack of T and B cell receptors, and the production of cytokines/chemokines that indicate an innate immune phenotype. iCD8α cells are a main source of osteoponin in the intestinal epithelium, a cytokine known for its role in tissue remodeling but also for its capacity to stimulate Th1 and Th17 immune responses. Interestingly, using a strain of mice lacking iCD8α cells (E8I mice; E8I is an essential enhancer for CD8αα expression in IEL) we found that the levels of -/- osteopontin in the intestinal epithelium are decreased in comparison to wild type mice, and that E8I-/- mice have a deficiency in NKp46+NK1.1+ IEL. These and other preliminary results have led to the hypothesis that iCD8α cells, through production of factors such as osteopontin, promote the survival and maintenance of IEL. In order to pursue this hypothesis, we propose the following complementary, yet independent aims: Aim1. To determine the contribution of iCD8α cells and osteopontin to IEL maintenance and survival. In this aim we will determine: a) the impact of iCD8α cells and osteopontin in the survival and proliferation of different IEL subsets; b) the impact of iCD8α cells and osteopontin in the immune environment of the intestines; and c) the role of osteopontin ligands in IEL homeostasis. Aim 2. To define the role of iCD8α cells and osteopontin during intestinal inflammation. In this section we will determine the role of iCD8α cells and osteopontin in three different disease models: a) intestinal inflammation by anti-CD40 antibody treatment, b) Citrobacter rodentium infection, and c) inflammation caused by adoptive transfer of effector T cells into immunodeficient mice. Aim 3. To determine the mechanisms controlling IEL homeostasis by iCD8α cells. Our previous publication indicates that IL-12 provides stimulus for activation of iCD8α cells. In this section we will determine a) the impact of IL-12 in activation of iCD8α cells, with implications on OPN secretion and IEL survival, and b) we will determine the gene expression signature of IEL responding to iCD8α cell/OPN stimulation.

总结