Developmental regulation of retinal microglia

视网膜小胶质细胞的发育调控

• 批准号: 10179402
• 负责人: Monica L Vetter
• 金额: $ 36.98万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10179402
• 关键词: ARHGEF5 gene; Address; Adult; Age; Apoptosis; Apoptotic; Architecture; Axon; BAX gene; Blindness; Blood Vessels; Brain; Cell Count; Cell Death; Cells; Complement; Complement Receptor; Cues; Data; Degenerative Disorder; Dependence; Development; Diphtheria Toxin; Disease; Embryo; Event; Flow Cytometry; Gene Expression; Gene Expression Profile; Gene Proteins; Generations; Genes; Goals; Health; Heterogeneity; ITGAX gene; Immune; In Situ Hybridization; Injury; Knockout Mice; Knowledge; Macrophage-1 Antigen; Maintenance; Maps; Mediating; Microglia; Molecular; Mus; Myelogenous; Nerve Degeneration; Neuroglia; Neuroimmune; Neurons; Pathologic; Pathway interactions; Phagocytes; Phenotype; Play; Process; Property; Reaction; Regulation; Resistance; Retina; Retinal Diseases; Retinal Ganglion Cells; Role; Shapes; Signal Pathway; Signal Transduction; Spatial Distribution; Stress; Synapses; Testing; Transgenic Mice; Up-Regulation; Variant; cell type; course development; genetic signature; insight; lipid metabolism; molecular phenotype; myelination; newborn neuron; postnatal; promoter; single-cell RNA sequencing; transcriptome sequencing

PROJECT SUMMARY Microglia, the resident neuroimmune cells of the CNS, play important developmental roles. While brain microglia have been extensively studied, less is known about the molecular properties and function of microglia in the developing retina. The goal of this proposal is to address how retinal microglia change over the course of development, the molecular pathways involved and how this relates to their function. The first aim will determine whether there are molecularly distinct subpopulations of microglia in embryonic and early postnatal retina. The second aim will address the function of microglia in the early postnatal retina by utilizing knowledge about their gene expression signature and targeting them for depletion. Finally, in the last aim we will test how developmental events govern retinal microglia phenotype and function, and the molecular pathways involved. By determining how changes in microglial properties are regulated and contribute to development of the retina we will gain insight into fundamental mechanisms of retina development and microglial function. This may ultimately inform our understanding of how microglia are modulated and participate in degenerative disease processes resulting in loss of vision.

项目总结