Field-Deployable Lab-on-a-Chip Nanosensing Platforms for Health and Environmental Monitoring

摘要：项目2 拟议的项目描述了一个全面的研究计划，以开发和应用自动化领域- 可部署的芯片上实验室纳米传感平台，具有高吞吐量、高灵敏度和高效率，可用于多个 超级基金中与健康和环境监测相关的危险物质的功能分析 研究中心(SRC)。由于广泛的人类活动在全球范围内构成了越来越多的环境挑战， 现代环境监测和分析技术对于保护人类变得尤为重要 减少有害工业污染物对健康的影响，并大大提高公众的认识。然而， 传统的以实验室为基础的分析仪器通常既昂贵又笨重，需要精心设计 由专职人员执行的操作程序。这项工作的目标是交付现场- 由新兴的微流体和纳米传感实现的可部署的多用途芯片上实验室技术 环境监测和人类健康方面的应用技术。具体地说，两个创新 将研究的技术平台是：1)用于高分辨率图像的微流控打印分析(MPA)系统 吞吐量高灵敏度生物分子分析(AIM I)，以及2)现场可部署的芯片上ELISA卡平台，以 结合定量纳米传感分子分析(AIMS II-IV)，从中可以获得3D可打印设备 定制并与移动设备连接，以进行健康和环境监测。在开发之后， 这些技术有望促进多路复用、定量、自动化的处理和分析 人体生物样品和环境样品，具有灵敏度高、周转快、成本低等特点。首字母 研究将涉及测试这些设备，以检测农药及其降解产物，作为已确定的 高度关注我们的社区合作伙伴。更广泛地说，这些平台将很容易适应商业 生物识别分子和大量超级基金优先化学品的检测。总体而言，这项研究 该项目直接推动了NIEHS开发检测危险物质的分析工具的任务 并将其应用于环境和人类健康监测应用。

ABSTRACT: PROJECT 2 The proposed project describes a comprehensive research plan to develop and apply automated field- deployable lab-on-a-chip nanosensing platforms with high throughput, sensitivity and efficiency for multi- functional analysis of hazardous substances relevant to health and environmental monitoring in the Superfund Research Center (SRC). As extensive human activities pose increased environmental challenges worldwide, modern environmental monitoring and analytical technologies become particularly important to protect human health from adverse exposure to industrial pollutants, and to considerably improve public awareness. However, conventional laboratory-based analytical instruments are typically expensive and bulky, and require elaborate operational procedures conducted by dedicated personnel. The objective of this work is to deliver field- deployable multipurpose lab-on-a-chip technologies enabled by emerging microfluidics and nanosensing technologies for application in environmental monitoring and human health. Specifically, two innovative technological platforms to be investigated are: 1) a microfluidic print-to-analyze (MPA) system for high- throughput high-sensitivity biomolecular analysis (Aim I), and 2) a field-deployable ELISA-on-a-chip platform to incorporate quantitative nanosensing molecular assays (Aims II-IV), from which a 3D printable device can be customized and interfaced with mobile devices for health and environmental monitoring. Upon development, these technologies are expected to facilitate multiplexed, quantitative, automated processing and analysis of human biospecimens and environmental samples, with high sensitivity, quick turnover at low cost. Initial studies will involve testing these devices to detect pesticides and their degradation products, as an identified high concern for our community partner. More broadly, these platforms will be easily adaptable to commercial biorecognition molecules and detection of numerous Superfund priority chemicals. Overall, this research project directly advances the NIEHS mandate to develop analytical tools for the detection of hazardous chemicals and to apply them to environmental and human health monitoring applications.