Effect of piezoelectric charges on oral microbiome modulation

压电电荷对口腔微生物组调节的影响

• 批准号: 10189941
• 负责人: Santiago Orrego
• 金额: $ 24.98万
• 依托单位: TEMPLE UNIV OF THE COMMONWEALTH
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-04 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10189941
• 关键词: Address; Affect; Ammonium; Anti-Bacterial Agents; Area; Bacteria; Barium; Biocompatible Materials; Biomass; Charge; Chemicals; Clinical; Clinical Services; Composite Dental Resin; Composite Resins; Dental; Dental Materials; Dental Restoration Failure; Dental caries; Dentin; Development; Economic Burden; Endodontics; Ensure; Environment; Excision; Experimental Designs; Failure; Filler; Formulation; Future; General Population; Generations; Goals; Grant; Growth; Health; Healthcare; In Vitro; Incidence; Ions; Life; Mastication; Measures; Metabolic; Microbial Biofilms; Microscopy; Modeling; Oral; Outcome; Pathogenicity; Patients; Pattern; Periodicity; Plant Resins; Property; Protocols documentation; Public Health; Quality of life; Recurrence; Research; Ribosomal RNA; Salivary; Sampling; Savings; Secondary Prevention; Sequence Analysis; Services; Silver; Streptococcus mutans; Surface; Technology; Time; Tissues; Tooth Diseases; Tooth Loss; Tooth structure; Translating; Underserved Population; Water; Work; Zinc Oxide; aged; biomaterial development; clinical practice; composite restoration; cost; dental resin; dysbiosis; improved; in vivo; innovation; mechanical properties; metatranscriptome; microbial; microbiome; microbiome composition; microbiome research; microorganism; models and simulation; nano; nanoparticle; oral biofilm; oral microbiome; pathogen; physical property; polymerization shrinkage; polymicrobial biofilm; premature; prevent; response; restoration; restorative dentistry; restorative material; seal; success; transcriptome sequencing

PROJECT SUMMARY Secondary caries is considered the leading cause of dental restorations failure. The economic burden of treating this dental condition is significant at a cost of over $5 billion each year in USA. Improving the seal of dental materials bonded to dental hard tissues is critical for the prevention of secondary caries, progression of dental disease and tooth loss. Removing bacteria at the bonded interface will prevent the (bio)chemical degradation of the bonded materials, and thus, increase the clinical service of restorations. A common limitation of current antibacterial technologies includes the short durability of the effects due to leaching and depletion of ions. Therefore, there is an immense need to develop a new generation of dental antibacterial materials to reduce and eliminate dental diseases at bonded interfaces (e.g. secondary caries). We recently developed a new composite with piezoelectric properties for antibacterial therapies by incorporating charged nano-fillers into dental resins. Our innovative approach significantly improves current technologies by using a single filler with long-lasting antibacterial effects. In this study, we propose to find the optimal amount of piezoelectric nano-filler to enable the antibacterial effect while retaining high mechanical and physical properties of the composite (aim 1). In addition, we will evaluate the oral microbiome-modulating effects of piezoelectric charges to understand the specific changes on the biofilm composition and identify the targeted species by piezoelectric charges (aim 2). We expect the development of the proposed technology to significantly impact the dental field by reducing the failure rate of composite restorations, in turn, reducing the costs associated with those failures, improving the health of patients, and preventing unnecessary loss of additional tooth structure.

项目摘要 继发龋被认为是导致牙齿修复失败的主要原因。的经济负担 在美国，治疗这种牙齿状况的费用每年超过50亿美元。改善密封性 结合到牙齿硬组织上的牙科材料对于预防继发性龋齿、进展 牙齿疾病和牙齿脱落的风险。去除粘合界面处的细菌将防止（生物）化学物质 粘合材料的降解，从而增加了粘合剂的临床应用。一个共同 当前抗菌技术的局限性包括由于浸出而导致的效果的短耐久性， 离子的耗尽。因此，迫切需要开发新一代的牙科抗菌剂 减少和消除粘结界面处的牙科疾病（例如继发性龋齿）的材料。我们最近 开发了一种新的复合材料与压电性能的抗菌治疗，通过纳入带电 纳米填充物到牙科树脂中。我们的创新方法通过使用 具有持久抗菌效果的单一填料。在这项研究中，我们建议找到最佳数量的 压电纳米填料，使抗菌效果，同时保持高的机械和物理性能 复合材料的性能（目标1）。此外，我们还将评估以下药物的口腔微生物群调节作用： 压电电荷，以了解生物膜组成的具体变化，并确定目标 物种的压电电荷（目标2）。我们希望所提出的技术的发展， 通过降低复合材料修复的失败率来显著影响牙科领域， 与这些失败相关的成本，改善患者的健康，并防止不必要的损失， 额外的牙齿结构。