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Omics/Clinical Core

组学/临床核心

基本信息

批准号：
10190233
负责人：
Jacques Ravel
金额：
$ 22.15万
依托单位：
UNIVERSITY OF MARYLAND BALTIMORE
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-04-20 至 2026-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10190233
关键词：
3-Dimensional Automobile Driving Bacteria Base Sequence Bioinformatics Biological Biomimetics Biopsy Catalogs Cell Line Cells Cervical Chlamydia trachomatis Clinical Clinical Research Communities DNA Data Data Analyses Data Set Data Store Engineering Ensure Environment Exposure to Genes Genetic Genital Genitalia Genomics Goals Guidelines High-Throughput Nucleotide Sequencing Human Human Microbiome Immune Immunity Infection Infrastructure Institutes Knowledge Laboratories Laboratory Research Laboratory Study Maintenance Maryland Metadata Microbiology Modeling Modernization Mucous Membrane Neisseria gonorrhoeae Protocols documentation Qualitative Evaluations RNA Reproducibility Research Resolution Resource Sharing Resources Rest Running Sampling Services Sexually Transmitted Diseases Specimen Structure Swab System Technology Testing Time Tissues Training United States National Institutes of Health Universities Vagina bacterial community body system cell type cervicovaginal chemokine cost cost effective cytokine data framework data resource data sharing experience genome sciences host microbiome host microbiota innovation medical schools metatranscriptomics microbiome microbiota novel open data pathogen programs response tool transcriptome transcriptome sequencing transcriptomics

项目摘要

PROJECT SUMMARY To support the goals of the SIM-STI Program, that aims at developing an innovative biomimetic model of the cervicovaginal environment comprising the microbiota to elucidate how the host-microbiota interactions are driving host functioning and response to infection by C. trachomatis or N. gonorrhoeae. To study these interactions requires technical approaches that describe the system from a broad perspective without prior knowledge to observe functioning of both the host, the microbiome and the pathogen(s), such as global transcriptomics (human host RNAseq and bacterial community transcriptomics [metatranscriptomics]), glycomics and in-depth immune cytokines and chemokines characterizations. Here we propose to apply these omics approaches to support Project 1, 2 and 3 of the SIM-STI program. Further, transferring our proposed 3D biomimetic models from the engineering laboratory to three research laboratories for the study of sexually transmitted infections requires consistent biological resources to insure reproducibility and comparability. Leveraging the expertise and experience of its PI and infrastructure of the Institute for Genome Sciences at the University fo Maryland School of Medicine, the Omics/Clinical Core C will support this goal and will assemble and distribute biological resources (primary cell lines and reconstructed microbiota) using consistent protocols and qualitative evaluation of the materials to Project 1, 2 and 3, as well as the Biomimetic Models Core B. The overall goal of the Omics/Clinical Core C is to 1/ apply high-throughput omics approaches to samples provided by each of the project; 2/ provide microbiological (reconstructed microbiota) and primary cell purchase, isolation and maintenance services; 3/ perform a clinical study to collect cervicovaginal specimens (swabs, secretion and biopsies) for microbiota characterization, bacterial isolation and primary cell lines isolation; and 4/ disseminate the data and resources to the rest of the team and ultimately the scientific community through the Open Science Data Framework (OSDF), an innovative and scalable platform to store data of different types along with their metadata and create relationships between the different datasets.

项目总结