timsTOF fleX with MALDI-2 for Advanced Mass Spectrometry Imaging

有了这笔高端仪器赠款(HEI)，我们申请资金购买一台Bruker TimsTOF FLEX MALDI-2 先进的基质辅助激光解吸/电离(MALDI)成像仪器。这种质谱学 成像(MSI)仪器能够快速、高空间分辨率、高光谱分离地进行MALDI成像， 这是通过捕获离子迁移率光谱(TIMS)与成像中的质谱学相结合而实现的 模式。该仪器配备了第二个用于MALDI-2的尖端后电离激光器，以 增强信号强度以提高灵敏度和空间分辨率。这些技术进步使 TimsTOF flex MALDI-2MSI仪器是一种具有前所未有的分子水平的吸引人的分子发现工具 用于可视化药物、代谢物、脂类、胰多肽、 蛋白质和组织切片中的N-糖链。TimsTOF FLEX MALDI-2将被放置在约翰·霍普金斯大学 医疗机构(JHMI)应用成像质谱学(AIMS)核心设施，建立并指导 由在MALDI有15年经验的HeI S10应用程序的首席研究员格伦德博士撰写 成像。一个由美国国立卫生研究院资助的10名研究人员组成的小组，以及格伦德博士，将组成一个专门的小组 主要用户，其项目将从布鲁克TIME TOF FLEX MALDI-2 MSI的供应中受益匪浅 乐器。每个主要用户都需要7-10%的仪器时间。这些主要用户由 NIH资助的14项R01/R21/R35研究基金，其研究成果的时间为TOF FLEX MALDI-2 这一工具将产生变革性的影响。它们分布在8个部门，其中包括 放射、肿瘤、药理学、儿科、传染病、生物化学、 神经学和整形外科。几个较小的用户也将请求仪器时间。大调和 未成年使用者在癌症、传染病、抗逆转录病毒药物、免疫疗法、 神经科学，儿科学，生物化学。其中三名用户来自东海岸的机构， 包括马里兰大学，纽约大学和斯克兰顿大学，证明了 JHMI AIMS核心作为一种地区性资源。放射科和JHMI已经承诺 为这一工具的运作提供大量机构资金，其中包括一流的基础设施 JHMI AIMS核心设施，以及支付仪器的运行成本，并提供专门的 技术人员帮助用户高效地进行样品制备、仪器设置和数据分析 他们各自的MALDI成像应用。可通过以下方式在线预订和支付此仪器的费用 AIMS核心中的iLab软件。

With this high-end instrumentation grant (HEI), we apply for funds to purchase a Bruker timsTOF fleX MALDI-2 instrument for advanced matrix-assisted laser desorption/ionization (MALDI) imaging. This mass spectrometry imaging (MSI) instrument is capable of fast, high-spatial-resolution, high-spectral separation MALDI imaging, which is enabled by trapped ion mobility spectrometry (TIMS) combined with mass spectrometry in imaging mode. This instrument comes with the cutting-edge addition of a second post-ionization laser for MALDI-2 to boost signal intensity for increased sensitivity and spatial resolution. These technological improvements make the timsTOF fleX MALDI-2 MSI instrument an attractive molecular discovery tool with unprecedented molecular depth and coverage for visualizing the spatial distributions of drugs, metabolites, lipids, tryptic peptides, proteins, and N-glycans from tissue sections. The timsTOF fleX MALDI-2 will be placed in the Johns Hopkins Medical Institutions (JHMI) Applied Imaging Mass Spectrometry (AIMS) Core facility, established and directed by Dr. Glunde, the principal investigator of this HEI S10 application, who has 15 years of experience in MALDI imaging. A group of 10 NIH-funded investigators, along with Dr. Glunde, will comprise a dedicated group of major users, whose projects will greatly benefit from the availability of the Bruker timsTOF fleX MALDI-2 MSI instrument. Each major user is requesting 7-10% of instrument time. These major users are supported by a total of 14 NIH-funded R01/R21/R35 research grants on whose research outcomes the timsTOF fleX MALDI-2 instrument would have a transformative impact. They are spread across 8 Departments, which include the Departments of Radiology, Oncology, Pharmacology, Pediatrics, Infectious Disease, Biological Chemistry, Neurology, and Plastic Surgery. Several minor users will be requesting instrument time as well. Major and minor users have diverse research projects on cancer, infectious disease, antiretroviral drugs, immunotherapy, neuroscience, pediatrics, biological chemistry. Three of the users are from institutions on the East Coast, including the University of Maryland, New York University, and the University of Scranton, demonstrating that the JHMI AIMS core serves as a regional resource. The Radiology Department and JHMI have committed significant institutional funds towards the operation of this instrument, which includes the superb infrastructure of the JHMI AIMS Core facility, as well as covering running costs for the instrument, and providing dedicated technical personnel to help users with efficient sample preparation, instrument set-up and data analysis for their respective MALDI imaging applications. Online booking and billing for this instrument is available through the iLAB software in the AIMS Core.