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Developing a Rapid, Simple-to-use Sensory Platform for Detection of Ultralow Concentration of SARS-CoV-2 Viral Particles Enabled by Electrophoretic Enhancement and Redox Cycling

开发快速、简单易用的传感平台，通过电泳增强和氧化还原循环检测超低浓度的 SARS-CoV-2 病毒颗粒

基本信息

批准号：
10195022
负责人：
SeyedehAida Ebrahimi
金额：
$ 59.08万
依托单位：
PENNSYLVANIA STATE UNIVERSITY, THE
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-21 至 2024-09-20
项目状态：
已结题

项目摘要

Abstract: COVID-19 caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) remains an extraordinary global health crisis in the modern history. Meeting the testing needs for clinical diagnosis remains an unmet global challenge. Simple-to-use, sensitive, and rapid diagnostics are therefore urgently needed for early diagnosis of infection. The objective of this research is to design and demonstrate proof-of-principle of a novel low-cost and simple-to-use electrochemical sensing platform to enable rapid, ultrasensitive and accurate detection of SARS-CoV-2 virions in saliva (with accuracy ≥ 90% and total assay time < 30 min). We propose to achieve high sensitivity through two complementary signal amplification schemes, by electrophoretic concentration of virus-magnetic nanoparticle (mNP) conjugates by applying a small voltage on a set of electrodes with sub-micrometer gap, and amplifying electrochemical current through redox cycling between the same set of electrodes. Importantly, the proposed platform is suitable for commercialization by leveraging a low-cost and scalable fabrication method to create the sensor arrays without using expensive and non-scalable nanofabrication techniques. Fast, sensitive, and accurate detection of viral particles enables better surveillance and control of spread of the infection. The proposed platform is simple-to-use and suitable for point-of-care applications by eliminating tedious RNA extraction steps as in RT-PCR methods. It can enable high-throughput testing by creating sensor array on the same chip with small footprint to simultaneously analyze a multitude of sample droplets. The proposed sensory platform can also be expanded to detect other infectious pathogens, including Dengue and Influenza viruses, bacterial pathogens, etc.

摘要：由严重急性呼吸综合征冠状病毒2型引起的新冠肺炎仍然是一种现代史上罕见的全球卫生危机。满足临床诊断检测需求的余地一项尚未得到满足的全球挑战。因此，迫切需要简单易用、敏感和快速的诊断方法感染的早期诊断。这项研究的目标是设计和演示一种新型低成本、易用的电化学传感平台，实现快速、超灵敏、准确唾液中SARS-CoV-2病毒粒子的检测(准确率≥为90%，总检测时间为30分钟)。我们建议通过两种互补的信号放大方案来实现高灵敏度，通过病毒-磁性纳米颗粒(MNP)偶联物的小电压电泳法浓缩一组具有亚微米间距的电极，通过氧化还原循环放大电化学电流在同一组电极之间。重要的是，拟议的平台适合通过以下方式商业化利用低成本和可扩展的制造方法来制造传感器阵列，而无需使用昂贵和不可扩展的纳米制造技术。快速、灵敏和准确的病毒颗粒检测能够更好地监测和控制病毒的传播感染。建议的平台简单易用，并适合于护理点应用，因为繁琐的RNA提取步骤，就像RT-PCR方法一样。它可以通过创建传感器来实现高通量测试阵列在同一芯片上，占地面积小，可同时分析大量样品液滴。这个建议的感官平台也可以扩展到检测其他传染性病原体，包括登革热和流感病毒、细菌病原体等。