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Developing a Rapid, Simple-to-use Sensory Platform for Detection of Ultralow Concentration of SARS-CoV-2 Viral Particles Enabled by Electrophoretic Enhancement and Redox Cycling

开发快速、简单易用的传感平台，通过电泳增强和氧化还原循环检测超低浓度的 SARS-CoV-2 病毒颗粒

基本信息

批准号：
10195022
负责人：
SeyedehAida Ebrahimi
金额：
$ 59.08万
依托单位：
PENNSYLVANIA STATE UNIVERSITY, THE
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-21 至 2024-09-20
项目状态：
已结题

项目摘要

Abstract: COVID-19 caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) remains an extraordinary global health crisis in the modern history. Meeting the testing needs for clinical diagnosis remains an unmet global challenge. Simple-to-use, sensitive, and rapid diagnostics are therefore urgently needed for early diagnosis of infection. The objective of this research is to design and demonstrate proof-of-principle of a novel low-cost and simple-to-use electrochemical sensing platform to enable rapid, ultrasensitive and accurate detection of SARS-CoV-2 virions in saliva (with accuracy ≥ 90% and total assay time < 30 min). We propose to achieve high sensitivity through two complementary signal amplification schemes, by electrophoretic concentration of virus-magnetic nanoparticle (mNP) conjugates by applying a small voltage on a set of electrodes with sub-micrometer gap, and amplifying electrochemical current through redox cycling between the same set of electrodes. Importantly, the proposed platform is suitable for commercialization by leveraging a low-cost and scalable fabrication method to create the sensor arrays without using expensive and non-scalable nanofabrication techniques. Fast, sensitive, and accurate detection of viral particles enables better surveillance and control of spread of the infection. The proposed platform is simple-to-use and suitable for point-of-care applications by eliminating tedious RNA extraction steps as in RT-PCR methods. It can enable high-throughput testing by creating sensor array on the same chip with small footprint to simultaneously analyze a multitude of sample droplets. The proposed sensory platform can also be expanded to detect other infectious pathogens, including Dengue and Influenza viruses, bacterial pathogens, etc.

摘要：由严重急性呼吸道综合征冠状病毒2型（SARS-CoV-2）引起的COVID-19仍然是一种现代史上最严重的全球健康危机满足临床诊断的测试需求仍然是一个尚未解决的全球性挑战。因此，迫切需要使用简单、灵敏和快速的诊断方法，感染的早期诊断。本研究的目的是设计和证明的原则，新型低成本和简单易用的电化学传感平台，能够快速，超灵敏和准确地检测唾液中的SARS-CoV-2病毒粒子（准确度≥ 90%，总检测时间< 30 min）。我们建议通过两种互补的信号放大方案来实现高灵敏度，通过在微电极上施加小电压来电泳浓缩病毒-磁性纳米颗粒（mNP）缀合物，一组具有亚微米间隙的电极，并通过氧化还原循环放大电化学电流在同一组电极之间。重要的是，所提出的平台适合商业化，利用低成本和可扩展的制造方法来创建传感器阵列，而不使用昂贵的不可扩展的纳米纤维技术。快速、灵敏和准确地检测病毒颗粒，可以更好地监测和控制病毒的传播。感染所提出的平台易于使用，并且通过消除如RT-PCR方法中冗长的RNA提取步骤。它可以通过创建传感器，在同一芯片上以较小的占地面积进行阵列，以同时分析大量样品液滴。的拟议的感官平台还可以扩展到检测其他传染性病原体，包括登革热和流感病毒、细菌病原体等