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Developing a Rapid, Simple-to-use Sensory Platform for Detection of Ultralow Concentration of SARS-CoV-2 Viral Particles Enabled by Electrophoretic Enhancement and Redox Cycling

开发快速、简单易用的传感平台，通过电泳增强和氧化还原循环检测超低浓度的 SARS-CoV-2 病毒颗粒

基本信息

批准号：
10195022
负责人：
SeyedehAida Ebrahimi
金额：
$ 59.08万
依托单位：
PENNSYLVANIA STATE UNIVERSITY, THE
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-21 至 2024-09-20
项目状态：
已结题

项目摘要

Abstract: COVID-19 caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) remains an extraordinary global health crisis in the modern history. Meeting the testing needs for clinical diagnosis remains an unmet global challenge. Simple-to-use, sensitive, and rapid diagnostics are therefore urgently needed for early diagnosis of infection. The objective of this research is to design and demonstrate proof-of-principle of a novel low-cost and simple-to-use electrochemical sensing platform to enable rapid, ultrasensitive and accurate detection of SARS-CoV-2 virions in saliva (with accuracy ≥ 90% and total assay time < 30 min). We propose to achieve high sensitivity through two complementary signal amplification schemes, by electrophoretic concentration of virus-magnetic nanoparticle (mNP) conjugates by applying a small voltage on a set of electrodes with sub-micrometer gap, and amplifying electrochemical current through redox cycling between the same set of electrodes. Importantly, the proposed platform is suitable for commercialization by leveraging a low-cost and scalable fabrication method to create the sensor arrays without using expensive and non-scalable nanofabrication techniques. Fast, sensitive, and accurate detection of viral particles enables better surveillance and control of spread of the infection. The proposed platform is simple-to-use and suitable for point-of-care applications by eliminating tedious RNA extraction steps as in RT-PCR methods. It can enable high-throughput testing by creating sensor array on the same chip with small footprint to simultaneously analyze a multitude of sample droplets. The proposed sensory platform can also be expanded to detect other infectious pathogens, including Dengue and Influenza viruses, bacterial pathogens, etc.

抽象的：由严重急性呼吸综合征冠状病毒-2 (SARS-CoV-2) 引起的 COVID-19 仍然是一种现代史上非同寻常的全球健康危机。满足临床诊断的检测需求仍待解决尚未解决的全球挑战。因此，迫切需要简单易用、灵敏且快速的诊断方法感染的早期诊断。本研究的目的是设计并论证原理验证新颖的低成本且易于使用的电化学传感平台可实现快速、超灵敏和准确唾液中 SARS-CoV-2 病毒体的检测（准确度 ≥ 90%，总检测时间 < 30 分钟）。我们建议通过两种互补的信号放大方案来实现高灵敏度，通过通过在电泳上施加小电压来电泳浓缩病毒-磁性纳米颗粒 (mNP) 缀合物一组具有亚微米间隙的电极，并通过氧化还原循环放大电化学电流在同一组电极之间。重要的是，所提出的平台适合商业化利用低成本且可扩展的制造方法来创建传感器阵列，而无需使用昂贵的和不可扩展的纳米制造技术。快速、灵敏且准确地检测病毒颗粒可以更好地监测和控制病毒的传播感染。所提出的平台易于使用，并且通过消除 RT-PCR 方法中繁琐的 RNA 提取步骤。它可以通过创建传感器来实现高通量测试阵列位于同一芯片上，占地面积小，可同时分析大量样品液滴。这所提出的传感平台还可以扩展到检测其他传染性病原体，包括登革热和流感病毒、细菌性病原体等