Developing a Rapid, Simple-to-use Sensory Platform for Detection of Ultralow Concentration of SARS-CoV-2 Viral Particles Enabled by Electrophoretic Enhancement and Redox Cycling
开发快速、简单易用的传感平台,通过电泳增强和氧化还原循环检测超低浓度的 SARS-CoV-2 病毒颗粒
基本信息
- 批准号:10195022
- 负责人:
- 金额:$ 59.08万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-09-21 至 2024-09-20
- 项目状态:已结题
- 来源:
- 关键词:2019-nCoVAccountingAcheAnimalsAntibodiesBiological AssayBusinessesCOVID-19COVID-19 detectionCOVID-19 early detectionCenters for Disease Control and Prevention (U.S.)Cessation of lifeClinicalCoronavirusDengue VirusDetectionDiagnosticEarly DiagnosisElectrodesElectrophoresisElectroplatingElementsFutureGoalsHealthcare SystemsHumanInfectionMagnetic nanoparticlesMagnetismMethodsModelingModern 1601-historyOxidation-ReductionPerformanceProtocols documentationRNARapid diagnosticsResearchResearch Project GrantsReverse Transcriptase Polymerase Chain ReactionSalivaSamplingSchemeSensitivity and SpecificitySensorySignal TransductionSpecificityTechniquesTestingTimeTranslationsValidationViralVirionVirusaccurate diagnosticsclinical Diagnosiscommercializationcoronavirus diseasecostcross reactivitydesigndetection limitdetection platformdiagnostic accuracydiagnostic platformelectric fieldglobal healthinfluenzavirusmeetingsnanofabricationnovelpandemic diseaseparticlepathogenpathogenic bacteriapoint of carerepositorysaliva samplesensorsimulationviral detectionvoltage
项目摘要
Abstract:
COVID-19 caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) remains an
extraordinary global health crisis in the modern history. Meeting the testing needs for clinical diagnosis remains
an unmet global challenge. Simple-to-use, sensitive, and rapid diagnostics are therefore urgently needed for
early diagnosis of infection. The objective of this research is to design and demonstrate proof-of-principle of a
novel low-cost and simple-to-use electrochemical sensing platform to enable rapid, ultrasensitive and accurate
detection of SARS-CoV-2 virions in saliva (with accuracy ≥ 90% and total assay time < 30 min).
We propose to achieve high sensitivity through two complementary signal amplification schemes, by
electrophoretic concentration of virus-magnetic nanoparticle (mNP) conjugates by applying a small voltage on a
set of electrodes with sub-micrometer gap, and amplifying electrochemical current through redox cycling
between the same set of electrodes. Importantly, the proposed platform is suitable for commercialization by
leveraging a low-cost and scalable fabrication method to create the sensor arrays without using expensive and
non-scalable nanofabrication techniques.
Fast, sensitive, and accurate detection of viral particles enables better surveillance and control of spread of the
infection. The proposed platform is simple-to-use and suitable for point-of-care applications by eliminating
tedious RNA extraction steps as in RT-PCR methods. It can enable high-throughput testing by creating sensor
array on the same chip with small footprint to simultaneously analyze a multitude of sample droplets. The
proposed sensory platform can also be expanded to detect other infectious pathogens, including Dengue and
Influenza viruses, bacterial pathogens, etc.
摘要:
由严重急性呼吸综合征冠状病毒2型引起的新冠肺炎仍然是一种
现代史上罕见的全球卫生危机。满足临床诊断检测需求的余地
一项尚未得到满足的全球挑战。因此,迫切需要简单易用、敏感和快速的诊断方法
感染的早期诊断。这项研究的目标是设计和演示一种
新型低成本、易用的电化学传感平台,实现快速、超灵敏、准确
唾液中SARS-CoV-2病毒粒子的检测(准确率≥为90%,总检测时间为30分钟)。
我们建议通过两种互补的信号放大方案来实现高灵敏度,通过
病毒-磁性纳米颗粒(MNP)偶联物的小电压电泳法浓缩
一组具有亚微米间距的电极,通过氧化还原循环放大电化学电流
在同一组电极之间。重要的是,拟议的平台适合通过以下方式商业化
利用低成本和可扩展的制造方法来制造传感器阵列,而无需使用昂贵和
不可扩展的纳米制造技术。
快速、灵敏和准确的病毒颗粒检测能够更好地监测和控制病毒的传播
感染。建议的平台简单易用,并适合于护理点应用,因为
繁琐的RNA提取步骤,就像RT-PCR方法一样。它可以通过创建传感器来实现高通量测试
阵列在同一芯片上,占地面积小,可同时分析大量样品液滴。这个
建议的感官平台也可以扩展到检测其他传染性病原体,包括登革热和
流感病毒、细菌病原体等。
项目成果
期刊论文数量(3)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Rapid and sensitive detection of viral particles by coupling redox cycling and electrophoretic enrichment.
- DOI:10.1016/j.bios.2022.114198
- 发表时间:2022-07-15
- 期刊:
- 影响因子:12.6
- 作者:Butler D;Ebrahimi A
- 通讯作者:Ebrahimi A
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