Developing a Rapid, Simple-to-use Sensory Platform for Detection of Ultralow Concentration of SARS-CoV-2 Viral Particles Enabled by Electrophoretic Enhancement and Redox Cycling

开发快速、简单易用的传感平台，通过电泳增强和氧化还原循环检测超低浓度的 SARS-CoV-2 病毒颗粒

• 批准号: 10195022
• 负责人: SeyedehAida Ebrahimi
• 金额: $ 59.08万
• 依托单位: PENNSYLVANIA STATE UNIVERSITY, THE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-21 至 2024-09-20
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10195022
• 关键词: 2019-nCoV; Accounting; Ache; Animals; Antibodies; Biological Assay; Businesses; COVID-19; COVID-19 detection; COVID-19 early detection; Centers for Disease Control and Prevention (U.S.); Cessation of life; Clinical; Coronavirus; Dengue Virus; Detection; Diagnostic; Early Diagnosis; Electrodes; Electrophoresis; Electroplating; Elements; Future; Goals; Healthcare Systems; Human; Infection; Magnetic nanoparticles; Magnetism; Methods; Modeling; Modern 1601-history; Oxidation-Reduction; Performance; Protocols documentation; RNA; Rapid diagnostics; Research; Research Project Grants; Reverse Transcriptase Polymerase Chain Reaction; Saliva; Sampling; Scheme; Sensitivity and Specificity; Sensory; Signal Transduction; Specificity; Techniques; Testing; Time; Translations; Validation; Viral; Virion; Virus; accurate diagnostics; clinical Diagnosis; commercialization; coronavirus disease; cost; cross reactivity; design; detection limit; detection platform; diagnostic accuracy; diagnostic platform; electric field; global health; influenzavirus; meetings; nanofabrication; novel; pandemic disease; particle; pathogen; pathogenic bacteria; point of care; repository; saliva sample; sensor; simulation; viral detection; voltage

Abstract: COVID-19 caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) remains an extraordinary global health crisis in the modern history. Meeting the testing needs for clinical diagnosis remains an unmet global challenge. Simple-to-use, sensitive, and rapid diagnostics are therefore urgently needed for early diagnosis of infection. The objective of this research is to design and demonstrate proof-of-principle of a novel low-cost and simple-to-use electrochemical sensing platform to enable rapid, ultrasensitive and accurate detection of SARS-CoV-2 virions in saliva (with accuracy ≥ 90% and total assay time < 30 min). We propose to achieve high sensitivity through two complementary signal amplification schemes, by electrophoretic concentration of virus-magnetic nanoparticle (mNP) conjugates by applying a small voltage on a set of electrodes with sub-micrometer gap, and amplifying electrochemical current through redox cycling between the same set of electrodes. Importantly, the proposed platform is suitable for commercialization by leveraging a low-cost and scalable fabrication method to create the sensor arrays without using expensive and non-scalable nanofabrication techniques. Fast, sensitive, and accurate detection of viral particles enables better surveillance and control of spread of the infection. The proposed platform is simple-to-use and suitable for point-of-care applications by eliminating tedious RNA extraction steps as in RT-PCR methods. It can enable high-throughput testing by creating sensor array on the same chip with small footprint to simultaneously analyze a multitude of sample droplets. The proposed sensory platform can also be expanded to detect other infectious pathogens, including Dengue and Influenza viruses, bacterial pathogens, etc.

文摘:

项目成果

Rapid and sensitive detection of viral particles by coupling redox cycling and electrophoretic enrichment.

• DOI: 10.1016/j.bios.2022.114198
• 发表时间: 2022-07-15
• 期刊: Biosensors & bioelectronics
• 影响因子: 12.6
• 作者: Butler D;Ebrahimi A
• 通讯作者: Ebrahimi A