Imaging Module

成像模块 摘要 成像模块的目的是为高分辨率、多通道提供仪器和培训 为视觉核心小组的成员提供成像和定量图像分析。我们努力获得 我们的共焦设备的最新技术，可在中央定位的核心中支持多达5个通道的标签 所有核心奖助金成员均可使用设施。在共焦设备中，我们将有三个蔡司共焦 显微镜(蔡司LSM 510 Meta、蔡司LSM 780和蔡司LSM 800，带Airycan(订购))外加一个 蔡司Apotome 2安装在Axio成像仪上。经过培训后，授权用户可以使用在线注册 Vision Core Grant网页上的日历，其中还包含说明和多渠道入门读物 共聚焦显微镜。凤凰研究OCT系统可用于小动物工作。一项倡议 提供双光子成像已经开始，并承诺提供支持，目标是使其可用 在该计划的第一年。共焦显微镜技术援助和培训，OCT和 图像分析可从模块支持的显微镜专家和软件专家处获得。节目 用于离线图像分析(ZenLite和ImageJ)广泛分布在整个用户组和 模块拥有几个附加程序(ZenBlue、Imaris和Image Pro)的许可证。该模块还将 为多通道成像提供一个全面的荧光二次抗体库。 优先使用的是NEI支持的研究和视觉研究的试点项目。因为影像 模块将支持对显微镜的服务合同，共焦显微镜将免费提供给所有人 愿景核心奖助金的成员免费。我们认为这是仔细分析 共焦材料。免费政策积极鼓励新用户和初级教师，因为它允许 是时候学习共焦成像了。

IMAGING MODULE ABSTRACT The purpose of the Imaging Module is to provide instrumentation and training for high-resolution, multi-channel imaging and quantitative image analysis to the members of the Vision Core Group. We strive to obtain the latest technology for our confocal facility, which can support up to 5-channel labeling in a centrally located core facility accessible to all Core Grant members. In the confocal facility, we will have three Zeiss confocal microscopes, (Zeiss LSM 510 META, Zeiss LSM 780 and Zeiss LSM 800 with Airyscan (on order)) plus a Zeiss Apotome 2 mounted on an Axio Imager. After training, authorized users may sign up using the on-line calendar on the Vision Core Grant web page, which also contains instructions and a primer on multi-channel confocal microscopy. A Phoenix Research OCT system is available for small animal work. An initiative to provide 2-photon imaging has been started with a commitment of support and a goal to make this available during the first year of the program. Technical assistance and training for confocal microscopy, OCT and image analysis are available from a microscopist and software specialist supported by the Module. Programs for off-line image analysis (ZenLite, and ImageJ) are widely distributed throughout the user group and the Module holds licenses for several additional programs (ZenBlue, Imaris and Image Pro). The Module will also provide access to a comprehensive library of fluorescent secondary antibodies for multi-channel imaging. Priority use is assigned to NEI supported research and pilot projects in vision research. Because the Imaging Module will support the service contracts on the microscopes, confocal microscopy will be freely available to all members of the Vision Core Grant at no charge. We believe this is a key factor in the careful analysis of confocal material. The no-charge policy actively encourages new users and junior faculty because it allows the time to learn about confocal imaging.