Exploring the role of mitochondria in dysregulated calcium handling in diseased hearts
Exploring the role of mitochondria in dysregulated calcium handling in diseased hearts
批准号:
10202296
负责人:
Bin Liu
金额:
$42.27万
依托单位国家:
美国
项目类别:
财政年份:
2021
资助国家:
美国
项目状态:
已结题
起止时间:
2021-06-10 至 2024-05-31
关键词:
AffectAnimalsArrhythmiaBiochemicalBiological AssayBuffersCalciumCardiacCatecholaminergic Polymorphic Ventricular TachycardiaCause of DeathCell DeathCell physiologyCellsChronicComplexDataDefectDevelopmentDiseaseDisease modelFosteringFoundationsFunctional disorderFutureGenerationsGeneticGenetic ModelsGoalsHeartHeart AbnormalitiesHeart DiseasesHeart failureHomeostasisImageInterventionKnowledgeLifeLinkLiteratureMediatingMembrane PotentialsMetabolicMetabolic DiseasesMethodsMitochondriaMolecularMusMuscle CellsMutationOutcomePathologicPathologyPharmaceutical PreparationsPhenotypePhosphorylationPhysiologicalPlayPrediabetes syndromeProductionProtein BiochemistryProteinsResearchRoleRyanodine Receptor Calcium Release ChannelRyanodine ReceptorsSarcoplasmic ReticulumShapesSignal TransductionStructureSyndromeTechniquesTestingTranslatingUnited Statesbasecalmodulin-dependent protein kinase IIcareerdiabetic cardiomyopathydisease phenotypegenetic approachheart functionheart rhythmin vivoindividualized medicineinsightmitochondrial permeability transition poremouse modelnovelnovel therapeuticsoxidationtranslational studyundergraduate student
中文摘要
在美国,心脏病仍然是导致死亡的主要原因。改变Ca释放
英文摘要
Cardiac disease remains the leading cause of death in the United States. Altered Ca release from the
sarcoplasmic reticulum (SR) due to genetic and acquired defects in Ca release channels, ryanodine receptors
(RyR2s), are thought to underlie a spectrum of devastating cardiac disorders, ranging from arrhythmias to heart
failure. RyR2 dysfunction, mainly manifested as an abnormally active (i.e. leaky) channel, leads to aberrant Ca
release (ACR). However, while the key role of ACR in contributing to various disease states is established, it
remains unclear as to why and how the same underlying defect, i.e. aberrant Ca release, results in different
pathological phenotypes in different disease settings. For instance, ACR causes cardiac arrhythmias without
pathological remodeling in catecholaminergic polymorphic ventricular tachycardia (CPVT), a life-threatening
genetic arrhythmia syndrome. In contrast, ACR is associated with both pathological remodeling and arrhythmias
in a metabolic disease model of pre-diabetic cardiomyopathy (pre-DC). This divergence of outcomes suggests
that factors in addition to leaky RyR2s are critical for translating aberrant RyR2 Ca release to a particular disease
state, however there is a gap in knowledge regarding the connection between abnormal myocyte Ca handling
and cardiac disease. Mitochondria sense intracellular Ca signals to mediate energy production and also cell
death. Recently, the interplay between SR and mitochondria has emerged as an important factor in the
development of different cardiac pathologies. Preliminary results from this study suggest that this interplay
shapes/impacts pathological phenotypes in settings of two distinct cardiac diseases: CPVT and pre-DC. Based
on these results as well as data in the literature, it is hypothesized that the interplay between SR and
mitochondria contributes to Ca-dependent cardiac disease phenotypes by modulating/shaping intracellular Ca
signals. To test this hypothesis, multiscale studies (from molecule to whole animal) that employ novel genetic
mice models and utilize methods of cellular physiology and protein biochemistry, along with in vivo cardiac
functional assays, are proposed. The overall goal of this study is to engage undergraduate students to: 1) define
the molecular players and factors that determine the specific manner as to how mitochondria respond to ACR to
shape intracellular Ca dynamics and contribute to cardiac pathologies in CPVT vs pre-DC settings, and 2) utilize
genetic approaches to explore the effect of directly modulating mitochondria Ca on cardiac pathology in both
disease settings. The proposed research is significant because it will greatly advance the understanding of SR-
mitochondria Ca signaling in the setting of CPVT and pre-DC, and thus foster the development of mechanism-
based therapies for these devastating cardiac diseases. It will also act as a foundation for future translational
studies to provide tailored therapies for subtypes of Ca-dependent cardiac disease. Moreover, this project will
provide undergraduate students with numerous opportunities to participate in research, thus fully preparing them
for scientific or biomedical related careers.
期刊论文(2)
专著(0)
科研奖励(0)
会议论文
Genetic Inhibition of Mitochondrial Permeability Transition Pore Exacerbates Ryanodine Receptor 2 Dysfunction in Arrhythmic Disease.
线粒体通透性过渡孔的遗传抑制加剧了心律失常疾病中ryanodine受体2功能障碍。
DOI:
10.3390/cells12020204
发表时间:
2023-01-04
期刊:
Cells
影响因子:
6
作者:
[]
通讯作者:
DOI:
10.1093/cvr/cvab324
发表时间:
2022-10-21
期刊:
Cardiovascular research
影响因子:
10.8
作者:
[]
通讯作者:
Broad spectrum β-lactamase inhibitors employing a Trojan horse mechanism to rescue β-lactams against multidrug-resistant Pseudomonas aeruginosa
-
批准号:10482577
-
项目类别:
-
资助金额:$100.0万
-
财政年份:2022
-
负责人:Bin Liu
-
依托单位:
Broad spectrum β-lactamase inhibitors employing a Trojan horse mechanism to rescue β-lactams against multidrug-resistant Pseudomonas aeruginosa
-
批准号:10588165
-
项目类别:
-
资助金额:$100.0万
-
财政年份:2022
-
负责人:Bin Liu
-
依托单位:
Novel Metallo-beta-lactamase Inhibitors
-
批准号:8334617
-
项目类别:
-
资助金额:$29.4万
-
财政年份:2011
-
负责人:Bin Liu
-
依托单位:
海外基金