Biocompatible Graphene Quantum Dots for Noninvasive Near-infrared Bioimaging

用于无创近红外生物成像的生物相容性石墨烯量子点

• 批准号: 10203022
• 负责人: Anton Naumov
• 金额: $ 41.01万
• 依托单位: TEXAS CHRISTIAN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10203022
• 关键词: Address; Adverse effects; Animal Experimentation; Animal Model; Animal Testing; Animals; Area; Biological; Biological Assay; Biosensing Techniques; Biotechnology; Cell Survival; Cells; Chemicals; Clinical; Complex; Contrast Media; Defect; Development; Diagnosis; Diagnostic; Drug Delivery Systems; Drug Transport; Exhibits; Fluorescence; Fluorescence Microscopy; Future; Glucosamine; Health; Human; Image; Image-Guided Surgery; In Situ; In Vitro; Light; Magnetic Resonance Imaging; Medical Students; Microscopic; Minor; Mus; Natural graphite; Near-infrared optical imaging; Operative Surgical Procedures; Ophthalmology; Optics; Organ; Oxides; Oxygen; Patients; Penetration; Pharmaceutical Preparations; Process; Property; Quantum Dots; Rare Earth Metals; Research; Science; Site; Slice; Structure; System; Techniques; Testing; Therapeutic; Time; Tissue imaging; Tissues; Toxic effect; UV Radiation Exposure; Visible Radiation; Visualization; Work; animal imaging; base; bioimaging; biomaterial compatibility; career; drug testing; feasibility testing; fluorescence imaging; fluorophore; functional group; graphene; image guided; image-guided drug delivery; imaging agent; imaging platform; in vivo; in vivo imaging; in vivo imaging system; infrared microscopy; luminescence; microwave electromagnetic radiation; mouse model; nanomaterials; near infrared dye; novel; oxidation; ozone exposure; physical property; quantum; real-time images; success; targeted treatment; therapeutic evaluation; tool; tumor; undergraduate student; uptake; water solubility

Project Summary/Abstract Near-infrared (NIR) imaging is one of the most rapidly developing perspective techniques in biomedical diagnostics enabling 10 - 100 times deeper tissue imaging than regular visible fluorescence techniques. There is a significant clinical need for such imaging: high-depth CT and MRI imaging strategies are complex and less suitable for low-depth biosensing and intraoperative applications. Furthermore, in animal studies NIR imaging can enable in situ fluorescence tracking of tested therapeutics or tumor visualization inside a living small animal. However, as NIR imaging field is still developing, there is a lack of biocompatible and multifunctional platforms suitable for imaging, sensing and even drug transport highly desired in animal studies. In this project we will develop and test the feasibility of a novel NIR imaging platform based on graphene quantum dots (GQDs) addressing the critical needs of NIR bioimaging: fluorescence in the NIR (above 950 nm) with NIR excitation, high biocompatibility, photostability, and capabilities for drug tracing and in vivo NIR imaging in live animal models. This will be implemented in 3 steps: (1) synthesizing NIR-emissive GQDs via bottom-up and top-down approaches and characterizing their optical and physical properties, (2) testing GQDs for NIR in vitro imaging and assessing their biocompatibility and (3) performing in vivo imaging of GQD NIR fluorescence in live animals as well as ex vivo organ tissue imaging quantifying GQD content in excised organs. We will concomitantly assess potential adverse effects to select non-toxic GQD candidates. Throughout this process a variety of synthesized GQD structures will undergo rigorous 3-step selection for the capabilities of (1) high yield NIR fluorescence, (2) high biocompatibility and NIR imaging in cells, (3) NIR imaging inside a live sedated animal with minimal/no toxicity. The selected GQD candidates will enable in vivo animal testing of therapeutic delivery with noninvasive real-time image tracking reducing the number of animals sacrificed. In a longer term, we expect these GQD platforms to become a basis of the noninvasive NIR in vivo sensing in patients. As a result, the proposed initiative will address several significant areas of biotechnology and its development will ultimately contribute to the improvement of human health.

项目总结/文摘

项目成果

Detection of Pancreatic Cancer miRNA with Biocompatible Nitrogen-Doped Graphene Quantum Dots.

• DOI: 10.3390/ma15165760
• 发表时间: 2022-08-20
• 期刊: Materials (Basel, Switzerland)

Automated Approach to In Vitro Image-Guided Photothermal Therapy with Top-Down and Bottom-Up-Synthesized Graphene Quantum Dots.

• DOI: 10.3390/nano13050805
• 发表时间: 2023-02-22
• 期刊: Nanomaterials (Basel, Switzerland)
• 作者: Lee B;Stokes GA;Valimukhametova A;Nguyen S;Gonzalez-Rodriguez R;Bhaloo A;Coffer J;Naumov AV
• 通讯作者: Naumov AV

Doped Graphene Quantum Dots as Biocompatible Radical Scavenging Agents.

• DOI: 10.3390/antiox12081536
• 发表时间: 2023-07-31
• 期刊: Antioxidants (Basel, Switzerland)

Cancer Therapeutic siRNA Delivery and Imaging by Nitrogen- and Neodymium-Doped Graphene Quantum Dots.

• DOI: 10.1021/acsbiomaterials.3c00369
• 发表时间: 2023-05
• 期刊: ACS biomaterials science & engineering
• 影响因子: 5.8
• 作者: Alina Valimukhametova;B. Lee;U. C. Topkiran;Klara Gries;R. Gonzalez-Rodriguez;J. Coffer;G. Akkaraju;A. Naumov