Structural variation in neuronal circuits as a basis for functional and behavioral individuality
Structural variation in neuronal circuits as a basis for functional and behavioral individuality
批准号:
10206473
负责人:
BASSEM A HASSAN
金额:
$318.7万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2021
资助国家:
美国
项目状态:
已结题
起止时间:
2021-05-01 至 2024-04-30
关键词:
AdultAnatomyAnimal BehaviorAnimal ModelAnimalsBehaviorBehavioralBehavioral AssayBiological AssayBiological ModelsBrainCalciumCellsCellular MorphologyCodeCognitiveCommunitiesComplexComputer ModelsDataData SetDefectDimensionsDiseaseDisease modelDorsalDrosophila genusDrosophila melanogasterElectron MicroscopyEnvironmental Risk FactorEvolutionExhibitsFemaleFoundationsFundingGenesGeneticGenetic ModelsGoalsHeadHumanImageIndividualIndividualityInjuryInterneuronsInvestigationKnowledgeLabelLightLinkLobeMammalsMapsMicroscopeModelingMonitorMonozygotic twinsMorphologyNatureNervous System controlNervous system structureNeuronsOdorsOrganismPhenotypePopulationProteinsResearchResearch PersonnelResolutionResourcesSensoryShapesSignal TransductionStimulusStochastic ProcessesStructureStructure-Activity RelationshipSynapsesSystemTechnologyTestingTranslatingVariantVisual system structureWeightWorkapproach behaviorcomparativeconnectomeconnectome dataflyin vivoin vivo calcium imagingindividual variationinformation processinginsightmaleneural networkneuronal circuitrynovel strategiesolfactory sensory neuronsoptogeneticspostsynapticpredictive modelingpreferencepresynapticreconstructionrelating to nervous systemresponsesexskillstooltraittwo-photonvoltage
中文摘要
项目总结
英文摘要
Project Summary
A fundamental gap in our knowledge of the nervous system is understanding how variations in wiring and
connectivity of neuronal circuits relate to variability in neural computations and behavior. This gap has arisen
because anatomical connectivity and function are typically studied separately. Here, we will assemble a team of
researchers with complementary skills to tackle this problem. We will combine several technologies developed
in our labs, including in vivo calcium imaging during behavior to study neuronal population activity during
perceptually-guided behaviors and high-throughput electron microscopy (EM) to extract the connectivity of an
underlying network essential for that behavior. To do so, we will use Drosophila melanogaster as a model system
because it has a powerful genetic toolkit, tractable number of neurons, is amenable to large-scale behavioral
screens, and is a realistic target for comparative whole-brain connectomics. This makes the fly an excellent
model to develop a comprehensive approach to characterize neuronal circuits.
We will apply our new approach to investigate how population codes, network connectivity, and structure-function
relationships differ between individuals. Although it is well known that individuals, as well as males and females,
exhibit variable behaviors, little is understood about how variations in neuronal wiring and connectivity relate to
variations in neural computation and ultimately behavior. In our first aim, we will compare population codes,
wiring, and connectivity between multiple isogenic individuals that exhibit differences in visually-guided approach
behavior. In a second aim, we will apply similar approaches to investigate differences in odor preference
behavior. We will test how stochastic brain asymmetry, weighting of sensory signals, and repertoire of local
interneurons influence computations within individual brains. Analyzing structure-function relationships across
individuals will examine the tradeoff between neuronal circuit precision and variability, and reveal how specific
variations shape information processing and behavior. We will generate models predicting neuronal function and
behavior from circuit wiring and neuronal structure. Our work will be among the first to compare whole-brain,
synaptic-resolution connectomes of multiple individuals to reveal fundamental constraints on functional network
organization and discover how circuit variability supports individuality.
期刊论文(7)
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DOI:
10.1098/rspb.2023.0555
发表时间:
2023-07-26
期刊:
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
影响因子:
4.7
作者:
[Easton-Calabria, August C., Thuma, Jessie A., Cronin, Kayleigh, Melone, Gigi, Laskowski, Madalyn, Smith, Matthew A. Y., Pasadyn, Cassandra L., de Bivort, Benjamin L., Crall, James D.]
通讯作者:
Crall, James D.
DOI:
10.21769/bioprotoc.4875
发表时间:
2023-11-05
期刊:
BIO-PROTOCOL
影响因子:
0.8
作者:
[Bengochea, Mercedes, Preat, Thomas, Hassan, Bassem]
通讯作者:
Hassan, Bassem
DOI:
10.1016/j.celrep.2023.112772
发表时间:
2023-07-25
期刊:
Cell reports
影响因子:
8.8
作者:
[]
通讯作者:
DOI:
10.3389/fnbeh.2021.777873
发表时间:
2021
期刊:
Frontiers in behavioral neuroscience
影响因子:
3
作者:
[Maloney RT]
通讯作者:
Maloney RT
DOI:
10.1080/15548627.2023.2179778
发表时间:
2023-10
期刊:
AUTOPHAGY
影响因子:
13.3
作者:
[Hassan, Bassem A., Hiesinger, P. Robin]
通讯作者:
Hiesinger, P. Robin
共 6 条
ATONAL AND MATH1 AND CNS AND PNS DEVELOPMENT
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批准号:6421083
-
项目类别:
-
资助金额:$3.08万
-
财政年份:2001
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负责人:BASSEM A HASSAN
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依托单位:
ATONAL AND MATH1 AND CNS AND PNS DEVELOPMENT
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批准号:6151526
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项目类别:
-
资助金额:$3.92万
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财政年份:2000
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负责人:BASSEM A HASSAN
-
依托单位:
ATONAL AND MATH1 AND CNS AND PNS DEVELOPMENT
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批准号:2775571
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项目类别:
-
资助金额:$3.02万
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财政年份:1999
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负责人:BASSEM A HASSAN
-
依托单位:
海外基金