American and Asian Centers for Arboviral Research and Enhanced Surveillance (A2CARES)

美国和亚洲虫媒病毒研究和加强监测中心 (A2CARES)

• 批准号: 10219913
• 负责人: Josefina Coloma
• 金额: $ 157.53万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-20 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10219913
• 关键词: Address; Africa; American; Americas; Arbovirus Infections; Arboviruses; Asia; Asian Americans; Asians; Basic Science; Chikungunya virus; Clinical; Clinical Data; Clinical Management; Clinical Research; Cohort Studies; Collaborations; Communicable Diseases; Communities; Comparative Study; Dengue; Dengue Virus; Detection; Diagnosis; Diagnostic; Diagnostic Procedure; Diagnostic tests; Disease; Disease Outbreaks; Ebola; Ecuador; Emerging Communicable Diseases; Epidemic; Epidemiology; Evaluation; Family; Foundations; Future; Geographic Locations; Geography; Goals; Health; Hospitals; Human; Immunology; In Situ; Incidence; Individual; Infection; Infectious Diseases Research; Infrastructure; Interdisciplinary Study; International; Investigation; Joints; Knowledge; Laboratories; Latin America; Location; Longitudinal cohort; Measures; Methods; Molecular; Molecular Epidemiology; National Institute of Allergy and Infectious Disease; Nicaragua; Orthobunyavirus; Outcome; Pattern; Performance; Phylogenetic Analysis; Publications; Readiness; Research; Research Infrastructure; Research Personnel; Rural; Rural Population; Sampling; Serology; Serology test; Severities; Severity of illness; Site; Specimen; Sri Lanka; Standardization; Statistical Data Interpretation; Sum; Surveys; System; Testing; Time; Training Programs; Vaccines; Viral; Viral Genome; ZIKA; Zika Virus; age group; authority; base; chikungunya; cohort; data management; design; emerging pathogen; experience; flexibility; human pathogen; innovation; member; method development; multiplex assay; next generation sequencing; nonhuman primate; novel; novel diagnostics; pandemic disease; pathogen; pathogenic virus; peri-urban; programs; response; surveillance data; sustainability framework; tool; transmission process; vaccine development; vector

ABSTRACT Recent pandemics of human pathogens have revealed the limitations of current surveillance systems. The sequential arboviral epidemics in the Americas showed how outbreak detection can be delayed and opportunities missed to collect well-characterized specimens and data for surveillance, basic science, clinical research, and development of vaccines and new diagnostics. In response, we have assembled a consortium of world-renowned investigators in arbovirology, epidemiology, immunology, viral diagnostics, phylogenetics, and clinical research, while leveraging research infrastructure and expertise in long-term cohort and hospital-based studies. This has resulted from 3 decades of collaborative international research, with over 200 joint publications, and extensive experience in preparing for and responding to outbreaks working closely with local and international health authorities and NIAID. With key sites in Asia and the Americas, we will use innovative molecular and serological methods to identify emerging pathogens and to address fundamental questions in dengue and other arboviral epidemiology and test viral, host and environmental determinants of differences between sites along a gradient of urbanicity. Our overarching goal is to develop an interconnected, harmonized network of clinical and laboratory sites to strengthen research programs, compare disease epidemiology and severity in different regions, develop and implement cutting-edge diagnostic methods, and respond efficiently and effectively to outbreaks. Standardized hospital studies and community-based cohorts in Ecuador, Nicaragua and Sri Lanka will characterize and compare human arboviral illnesses across urban, peri-urban and rural sites and develop sustainable infrastructure to rapidly respond to epidemics together with Ministries of Health, comple- mented by outbreak investigation, surveillance of non-human primates, and vector incrimination. We will imple- ment standardized plans for study administration, data and clinical management, and statistical analysis across sites, supported by an extensive on-site training program. Aim 1 will establish standard and novel multiplex assays at each site for surveillance, diagnosis and research of arboviruses and other pathogens. Aim 2 com- pares dengue/arbovirus epidemiology and transmission in cohort studies located in ecologically distinct regions over a continuum of urbanicity. In Aim 3, we will characterize complete viral genome sequences for comparative studies of arboviral phylogenetics, phylogeography and molecular epidemiology. Aim 4 consists of surveillance, identification, and characterization of novel and unrecognized human pathogens from severe hospitalized cases, unexpected outbreaks, vectors and non-human primates. Molecular and serological assays will be developed for newly identified pathogens and quickly implemented across study sites to characterize the epidemiology and clinical manifestations and will be made available to the EIDRC network. In sum, our A2CARES Consortium will provide valuable new tools and knowledge and 3 interconnected centers in Latin America and South Asia that will be able to respond to outbreaks and to study emerging and endemic infectious diseases far into the future.

摘要 最近人类病原体的大流行揭示了当前监测系统的局限性。的 美洲连续发生的虫媒病毒疫情表明，疫情的发现可能会被推迟， 错过了收集用于监测、基础科学、临床研究的充分表征的标本和数据， 开发疫苗和新的诊断方法。作为回应，我们召集了一个世界知名的 虫媒病毒学、流行病学、免疫学、病毒诊断学、遗传学和临床研究方面的研究人员， 同时利用研究基础设施和长期队列和医院研究的专业知识。这 经过30年的国际合作研究，有200多份联合出版物， 与当地和国际卫生部门密切合作，准备和应对疫情的经验 当局和NIAID。在亚洲和美洲的主要地点，我们将使用创新的分子和血清学方法， 确定新出现的病原体并解决登革热和其他虫媒病毒感染中的基本问题的方法 流行病学和测试病毒，宿主和环境决定因素的差异之间的网站沿着梯度 城市化。我们的总体目标是建立一个相互联系、协调的临床和 加强研究项目，比较疾病流行病学和严重程度， 不同的地区，开发和实施先进的诊断方法，并有效地应对， 有效的爆发。厄瓜多尔、尼加拉瓜的标准化医院研究和社区队列研究 斯里兰卡将在城市、城郊和农村地区描述和比较人类虫媒病毒疾病 发展可持续的基础设施，与卫生部一道迅速应对流行病， 通过疫情调查、非人灵长类动物监测和病媒鉴定，我们将执行- 为研究管理、数据和临床管理以及统计分析制定标准化计划， 现场，由广泛的现场培训计划支持。目标1将建立标准的和新的多路复用 在每个地点进行虫媒病毒和其他病原体的监测、诊断和研究。目标2 COM- 在生态不同地区进行的队列研究中的登革热/虫媒病毒流行病学和传播 城市化的连续性。在目标3中，我们将表征完整的病毒基因组序列，用于比较。 虫媒病毒遗传学、虫媒地理学和分子流行病学研究。目标4包括监督， 鉴定和表征来自严重住院病例的新的和未识别的人类病原体， 意外爆发、病媒和非人类灵长类动物。将开发分子和血清学检测方法 用于新发现的病原体，并在研究中心迅速实施，以表征流行病学， 临床表现，并将提供给EIDRC网络。总而言之，我们的A2 CARES联盟将 提供有价值的新工具和知识，并在拉丁美洲和南亚建立3个相互联系的中心， 将能够应对疫情，并研究未来的新兴和地方性传染病。