Histology, Microscopy and Image Analysis Core

组织学、显微镜和图像分析核心

• 批准号: 10219293
• 负责人: JAMES J TOMASEK
• 金额: $ 14.8万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10219293
• 关键词: Animal Model; Animals; Archives; Biomedical Research; Budgets; Businesses; Cancer Center; Cell physiology; Cells; Centers of Research Excellence; Complement; Computer software; Confocal Microscopy; Control Animal; Core Facility; Cryopreserved Tissue; Development; Diabetes Mellitus; Doctor of Philosophy; Ensure; Equipment; Floor; Freezing; Funding; Goals; Grant; Growth; Health Sciences; Histologic; Histology; Image; Image Analysis; In Vitro; Laboratories; Lasers; Letters; Mentors; Methodology; Methods; Microscope; Microscopic; Microscopy; Nomarski Interference Contrast Microscopy; Oklahoma; Organ; Paraffin; Paraffin Embedding; Pathologic; Phase; Phase-Contrast Microscopy; Pilot Projects; Postdoctoral Fellow; Preparation; Publications; Research; Research Personnel; Scanning; Science; Scientist; Services; Slide; Stains; Technology; Tissue Banks; Tissues; Training; Universities; Wages; animal tissue; cost effective; design; diabetic; graduate student; human tissue; instrumentation; laboratory equipment; laser capture microdissection; live cell imaging; microscopic imaging; multi-photon; novel; quantitative imaging; response; sample fixation; tissue preparation; tissue processing; tool; translational cancer research

PROJECT SUMMARY The Histology, Microscopy and Image Analysis Core is designed to meet the needs of Promising Junior Investigators, Pilot Project Researchers, and Funded Diabetes Investigators by assisting them in studying diabetic human and animal tissues utilizing state-of-the-art histologic and microscopic technologies, as well as assisting them with and providing them with a variety of types of microscopy required for in vitro cellular microscopic analysis. Tissue preparation requires a specialized laboratory and equipment, while state-of-the-art microscopes are outside the budget of most laboratories. The objective of the HMIA Core is twofold. One is to centralize histological preparation of tissues and provide access to specialized equipment for preparing and cutting tissues. The second is to maintain and provide training on and access to a variety of different types of state-of-the-art microscopes and to quantitative image analysis software. The core will provide tissue processing, embedding, sectioning, and histochemical staining of mounted slides, for both paraffin embedded and cryo-preserved tissue preparations. In addition, the core will provide quality state-of-the-art instrumentation and expertise for investigators to obtain microscopic images of publication quality using bright field, phase contrast, and Nomarski DIC microscopy, epifluorescence microscopy, confocal and multi-photon microscopy, laser capture microdissection microscopy, and live-cell imaging microscopy. To complement the microscopy component of this Core, assistance and training with a variety of quantitative image analysis tools is provided. The signup, billing, and tracking of Core use is all done using iLabs software. The Core comprises 500 sq ft on the 5th floor of the Biomedical Sciences Building for the histology functions and 150 sq ft on the 2nd floor of the Biomedical Research Building for imaging functions. A business plan is in place so that by the end of the COBRE Phase III the core will be financially stable and ready to integrate into the OUHSC Research Core Facilities network.

项目概要 组织学、显微镜和图像分析核心旨在满足以下需求： 有前途的初级研究人员、试点项目研究人员和资助的糖尿病研究人员 通过利用最先进的技术帮助他们研究糖尿病人类和动物组织 组织学和显微技术，以及协助他们并为他们提供 体外细胞显微分析所需的各种类型的显微镜。组织 制备需要专门的实验室和设备，而最先进的显微镜 超出了大多数实验室的预算。 HMIA Core 的目标是双重的。一是 集中组织的组织学制备并提供专用设备 准备和切割组织。第二个是维持并提供培训和访问 各种不同类型的最先进的显微镜和定量图像分析 软件。核心将提供组织处理、包埋、切片和组织化学 固定载玻片的染色，适用于石蜡包埋和冷冻保存的组织制剂。 此外，该核心还将提供优质的最先进的仪器和专业知识 研究人员使用明场、相位获得出版质量的显微图像 对比、Nomarski DIC 显微镜、落射荧光显微镜、共焦和多光子 显微镜、激光捕获显微切割显微镜和活细胞成像显微镜。到 通过各种方式补充该核心的显微镜组成部分、协助和培训 提供定量图像分析工具。核心使用的注册、计费和跟踪都是如此 使用 iLabs 软件完成。核心区位于生物医学中心 5 楼，占地 500 平方英尺 科学大楼用于组织学功能和生物医学二楼 150 平方英尺 成像功能研究大楼。商业计划已经到位，以便到年底 COBRE 第三阶段核心将财务稳定并准备好融入 OUHSC 研究核心设施网络。