Histology, Microscopy and Image Analysis Core

组织学、显微镜和图像分析核心

• 批准号: 10219293
• 负责人: JAMES J TOMASEK
• 金额: $ 14.8万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10219293
• 关键词: Animal Model; Animals; Archives; Biomedical Research; Budgets; Businesses; Cancer Center; Cell physiology; Cells; Centers of Research Excellence; Complement; Computer software; Confocal Microscopy; Control Animal; Core Facility; Cryopreserved Tissue; Development; Diabetes Mellitus; Doctor of Philosophy; Ensure; Equipment; Floor; Freezing; Funding; Goals; Grant; Growth; Health Sciences; Histologic; Histology; Image; Image Analysis; In Vitro; Laboratories; Lasers; Letters; Mentors; Methodology; Methods; Microscope; Microscopic; Microscopy; Nomarski Interference Contrast Microscopy; Oklahoma; Organ; Paraffin; Paraffin Embedding; Pathologic; Phase; Phase-Contrast Microscopy; Pilot Projects; Postdoctoral Fellow; Preparation; Publications; Research; Research Personnel; Scanning; Science; Scientist; Services; Slide; Stains; Technology; Tissue Banks; Tissues; Training; Universities; Wages; animal tissue; cost effective; design; diabetic; graduate student; human tissue; instrumentation; laboratory equipment; laser capture microdissection; live cell imaging; microscopic imaging; multi-photon; novel; quantitative imaging; response; sample fixation; tissue preparation; tissue processing; tool; translational cancer research

PROJECT SUMMARY The Histology, Microscopy and Image Analysis Core is designed to meet the needs of Promising Junior Investigators, Pilot Project Researchers, and Funded Diabetes Investigators by assisting them in studying diabetic human and animal tissues utilizing state-of-the-art histologic and microscopic technologies, as well as assisting them with and providing them with a variety of types of microscopy required for in vitro cellular microscopic analysis. Tissue preparation requires a specialized laboratory and equipment, while state-of-the-art microscopes are outside the budget of most laboratories. The objective of the HMIA Core is twofold. One is to centralize histological preparation of tissues and provide access to specialized equipment for preparing and cutting tissues. The second is to maintain and provide training on and access to a variety of different types of state-of-the-art microscopes and to quantitative image analysis software. The core will provide tissue processing, embedding, sectioning, and histochemical staining of mounted slides, for both paraffin embedded and cryo-preserved tissue preparations. In addition, the core will provide quality state-of-the-art instrumentation and expertise for investigators to obtain microscopic images of publication quality using bright field, phase contrast, and Nomarski DIC microscopy, epifluorescence microscopy, confocal and multi-photon microscopy, laser capture microdissection microscopy, and live-cell imaging microscopy. To complement the microscopy component of this Core, assistance and training with a variety of quantitative image analysis tools is provided. The signup, billing, and tracking of Core use is all done using iLabs software. The Core comprises 500 sq ft on the 5th floor of the Biomedical Sciences Building for the histology functions and 150 sq ft on the 2nd floor of the Biomedical Research Building for imaging functions. A business plan is in place so that by the end of the COBRE Phase III the core will be financially stable and ready to integrate into the OUHSC Research Core Facilities network.

项目摘要 组织学、显微镜和图像分析核心旨在满足以下需求 有前途的初级研究者，试点项目研究者和资助的糖尿病研究者 通过协助他们利用最先进的技术研究糖尿病人类和动物组织 组织学和显微镜技术，以及协助他们，并为他们提供 体外细胞显微镜分析所需的各种类型的显微镜。组织 制备需要专门的实验室和设备，而最先进的显微镜 超出了大多数实验室的预算。HMIA核心的目标是双重的。一是 集中组织的组织学制备，并提供专用设备， 准备和切割组织。第二是保持和提供关于和获得一个 各种不同类型的最先进的显微镜和定量图像分析 软件核心将提供组织处理，包埋，切片和组织化学 石蜡包埋和冷冻保存组织制备物的固定载玻片染色。 此外，核心将提供高质量的最先进的仪器和专业知识， 研究人员获得出版物质量的显微图像，使用明场，相位 对比度，Nomarski DIC显微镜，落射荧光显微镜，共聚焦和多光子 显微镜、激光捕获显微切割显微镜和活细胞成像显微镜。到 补充本核心的显微镜组成部分，援助和培训与各种 提供了定量图像分析工具。注册、计费和跟踪核心服务器的使用， 使用iLabs软件。核心包括500平方英尺的5楼的生物医学 科学大楼的组织学功能和150平方英尺的二楼的生物医学 成像功能的研究大楼。一个商业计划已经到位，以便在年底前， COBRE第三阶段的核心将是财务稳定，并准备纳入OUHSC 研究核心设施网络。