Small Animal Imaging Core

摘要-小动物成像核心 在科布雷第一阶段建立的小动物成像核心(SAIC) 俄克拉荷马大学健康科学中心(OUSHC)提供最先进的 用于评估肿瘤生长、进展、转移和转移的小动物成像方法 对治疗的反应。国家工商行政管理总局的职能包括在以下方面提供援助和咨询 设计涉及分子成像的体外和体内研究。上汽集团工作人员提供 将他们的专业知识提供给科布雷首席初级调查员(PJI)，试点项目初级 调查人员(PPI)，以及来自OUHSC和邻近机构的其他调查人员 需要非侵入性分子成像的动物研究；提供使用 成像设备；以及随后的数据收集和分析。上汽集团还提供 协助优化成像和其他成像前和成像后的外科程序。在……里面 此外，上汽集团还提供先进的高含量活细胞成像技术。此外，上汽集团还开发了一款 带有分子标签的人和小鼠癌细胞的细胞库，这些标签被注释和 特色化的。使用这些细胞系消除了实验结果的变异性，并 保持严谨。最后，上汽集团员工每年都要接受软件更新等方面的培训 与成像相关的技术，使他们与成像技术的进步保持同步。 在第一阶段科布雷资助期间，4名PJI，2名PPI和等调查人员 接受过使用各种设备的服务和/或培训，并已进入上汽集团 进行他们研究的设施。这导致获得了重要的科学数据和 推进研究项目，形成会议报告、同行评议的出版物， 并提交拨款申请。最后，上汽集团继续扩大工厂规模，增加了 因此，新设备使调查人员能够进行尖端研究。 在第二阶段，国家工商总局计划继续培训和援助新的PJI， PPIS和其他研究人员轻松成功地进行了动物研究 严密性和可重复性。为了实现这些目标，上汽集团确定了三个具体目标 -1)在相关的体外和体内研究设计方面提供服务、帮助和咨询 用于分子成像；2)提供标记细胞用于监测基因表达、细胞转运、 以及体外和体内模型的疾病进展；3)协助高通量和高通量 活细胞的内容成像以及肿瘤异种移植和其他组织的成像。 SAIC服务的持续提供将使PJI、PPI和其他调查人员能够 生成用于提交拨款申请和获得研究资金的初步数据。

Abstract - Small Animal Imaging Core The Small Animal Imaging Core (SAIC) established under the Phase I COBRE at the University of Oklahoma Health Sciences Center (OUSHC) provides access to state of the art small animal imaging modalities for evaluating tumor growth, progression, metastasis, and response to therapy. The functions of the SAIC include providing assistance, and consultation in designing in vitro and in vivo studies that involve molecular imaging. The SAIC staff's provide their expertise to the COBRE principal junior investigators (PJIs), pilot project junior investigators (PPIs), and other investigators from OUHSC and neighboring institutions with animal studies that require non-invasive molecular imaging; provide training in the use of the imaging equipment's; and subsequent collection and analysis of data. SAIC also provides assistance in optimizing imaging and other pre- and post-imaging surgical procedures. In addition, SAIC provides advanced high-content live cell imaging. Further, SAIC has developed a cell bank of human and murine cancer cell lines with molecular tags that are annotated and characterized. Using these cell lines eliminates variability in the experimental results and maintain rigor. Finally, SAIC staff's undergo annual training for software updates and other imaging-related techniques to keep them abreast of advances in imaging. During the Phase I COBRE funding period, 4 PJIs, 2 PPIs, and 89 other investigators have received service and/or training in using various equipment's and have accessed the SAIC facility for conducting their research. This has led in obtaining significant scientific data and advancing research projects resulting in conference presentations, peer-reviewed publications, and grant application submissions. Finally, SAIC has continued to expand the facility by adding new equipment's thus enabling investigators in conducting cutting-edge research. In the Phase II period, the SAIC plans continuing to train and offer assistance to new PJIs, PPISs, and other investigators in successfully conducting animal studies with ease and have rigor and reproducibility. For achieving these objectives, SAIC has identified three specific aims – 1) provide service, assistance, and consultation in designing in vitro and in vivo studies related to molecular imaging; 2) provide labeled cells for monitoring gene expression, cell trafficking, and disease progression in in vitro and in vivo models; 3) Assist in high-throughput and high- content imaging of live cells and in imaging of tumor xenografts and other tissues. Continued availability of SAIC services will allow PJIs, PPIs, and other investigators in generating preliminary data for submitting grant applications and obtaining research funding.