ShEEP Request for CytoViva 3D Darkfield Hyperspectral Microscope

在用于治疗各种人类疾病的药物开发和药物递送领域中的挑战之一 缺乏对限制药物功效的固有细胞屏障的理解。另一个挑战 进行分子的细胞内运输及其在细胞中的定位的研究人员所遇到的 细胞器是在使用荧光标记，扭曲这些标签的负面影响的研究结果 在样品上。此外，缺乏足够的仪器限制了研究者进行 在自己的研究所进行尖端研究因此，他们不得不将材料运送到 机构/大学，这些机构/大学耗时、昂贵，并且不让研究者了解 结果很快。因此，通过获得适当的技术，不断扩大研究基础设施， 设备将促进研究人员的研究活动，并导致新的发现。 为了克服这些限制，我们正在申请资金购买CytoViva 3D增强型 用于增强俄克拉荷马州研究人员进行的研究的暗场高光谱显微镜 城市卫生保健系统。该系统提供高信噪比的3D增强暗场光学 高光谱成像，其专门设计用于在无标记或无标记环境中对无标记颗粒/分子进行成像， 荧光标记的活细胞和离体组织基质。显微镜的独特专利技术 允许可视化和监测颗粒，药物，生物制剂和细胞在溶液中和半透明 基质（细胞、组织、生物体）。因此，研究材料（药物、纳米颗粒、细胞-蛋白质）结合的研究是不可能的。 颗粒相互作用，抗体与细胞的结合），受体介导的结合和进入细胞（纳米颗粒， 量子点、病毒、细菌、金属、抗体）和细胞内定位和运输（siRNA、质粒 DNA、纳米颗粒、病毒、细菌、孢子）通过各种细胞内细胞器的生物学行为可以在体外测定， 在离体组织外植体中进行无标记成像并捕获图像。配备必要计算机的系统 软件系统提供成像的生物和非生物材料的定量光谱分析。的 协议可以自动化，使用户可以“离开”仪器，并执行其他实验。 最重要的是，无论是俄克拉荷马州市退伍军人医疗保健系统还是附属大学， 俄克拉荷马州健康科学中心（OUHSC）就有这样一种仪器，它可以实现高精度 分析细胞或离体组织中的无标记材料。因此，这是一个真正独特和宝贵的 资源证明它购买作为资源的研究人员进行现有的和新的研究领域， 俄克拉荷马州市退伍军人医疗保健系统。这一设备的提供将加强正在进行的研究， 建立新的研究领域，使为军队和退伍军人服务的男女受益。 该设备将由六个主要用户和一些未来的VA用户谁拥有六个VA优点 资助了三个高级职业科学家奖和一个职业发展奖。

One of the challenges in areas of drug development and drug delivery for treating various human diseases is the lack of understanding of the inherent cellular barriers that restrict the drug efficacy. Another challenge encountered by researchers conducting intracellular trafficking of molecules and their localization in cellular organelles is in the use of fluorescent labels that distort the study results with negative influence of these labels on samples. Further, lack of availability of adequate instrumentation restricts investigators from conducting cutting-edge research at their own institute. As a result they have to ship their materials to institutions/universities which are time-consuming, expensive, and do not give the investigator to know the results quickly. Therefore, continuous expansion of research infrastructure by acquiring appropriate equipment’s will enhance research activities among research investigator and lead to new discoveries. To override some of these limitations, we are requesting funds to purchase the CytoViva 3D Enhanced Darkfield Hyperspectral Microscope for enhancing the research conducted by investigators at the Oklahoma City VA Health Care System. This system offers high signal-to-noise 3D enhanced darkfield optical hyperspectral imaging specifically designed for imaging of label free particles/molecules in label-free or fluorescently labeled live cells and ex-vivo tissue matrices. The unique patented technology of the microscope allows the visualization and monitoring of particles, drugs, biologics, and cells in solution and in translucent matrices (cells, tissues, organisms). Thus, studies investigating binding of material (drug, nanoparticle, cell- particle interaction, antibody binding to cell), receptor-mediated binding and entry into cells (nanoparticles, quantum-dots, virus, bacteria, metals, antibodies) and intracellular localization and trafficking (siRNA, plasmid DNA, nanoparticles, virus, bacteria, spores) via various intracellular organelles can be determined in vitro and in ex-vivo tissue explants with label-free imaging and capture images. The system with the requisite computer software system provides quantitative spectral analysis of biological and non-biological materials imaged. The protocols can be automated giving the user to ‘walk-away’ from the instrument, and perform other experiments. Most importantly, neither the Oklahoma City VA Health Care System nor the affiliated University of Oklahoma Health Sciences Center (OUHSC) has such an instrument that enables high precision analysis of label-free materials in cells or ex-vivo tissues. Therefore, this is a truly unique and valuable resource justifying it purchase as a resource for investigators to conduct existing and new areas of research at the Oklahoma City VA Health Care System. Availability of this equipment will enhance ongoing studies and establish new areas of research benefitting the men and women serving the military and veterans. The equipment will be used by six-major users and a number of future VA users who have six VA Merit grants funded, three senior career scientist awards, and one career development award funded.