Preclinical Core

临床前核心

• 批准号: 10224311
• 负责人: SHERYL S MOY
• 金额: $ 31.94万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-28 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10224311
• 关键词: Angelman Syndrome; Animal Model; Architecture; Ataxia; Atlases; Attention deficit hyperactivity disorder; Automatic Data Processing; Behavior; Behavioral; Biological Assay; Brain; Brain imaging; CRISPR/Cas technology; Caliber; Center for Translational Science Activities; Childhood; Clinical; Cognitive; Consultations; Contrast Media; Core Facility; Data; Data Analytics; Data Science Core; Diagnosis; Drug Screening; Electrophysiology (science); Embryo; Epilepsy; Evaluation; Faculty; Functional disorder; Genes; Genetic; Goals; Grain; Human; Image; Imaging Device; In Vitro; Infrastructure; Intellectual and Developmental Disabilities Research Centers; Intellectual functioning disability; Intervention; Joubert syndrome; Laboratories; Machine Learning; Magnetic Resonance Imaging; Maps; Measurement; Methods; Microscopy; Microtus; Modeling; Molecular; Morphology; Motor; Mus; Neonatal; Neurobiology; Neurodevelopmental Disorder; Neuroglia; Neurons; Neurosciences; Phenotype; Photosensitivity; Pitt-Hopkins syndrome; Pre-Clinical Model; Prevention; Regimen; Research; Research Personnel; Research Training; Resolution; Resources; Rett Syndrome; Science; Sensory; Services; Social Functioning; Source; Structure; Structure-Activity Relationship; Students; Supervision; System; Techniques; Technology; Testing; Time; Tissues; Training; Work; animal imaging; behavior test; behavioral phenotyping; behavioral response; brain behavior; brain cell; brain volume; cognitive function; data access; data analysis pipeline; developmental disease; drug discovery; efficacy testing; electrical microstimulation; emotional functioning; genome editing; improved; in vivo; innovation; laboratory experience; laboratory facility; member; method development; migration; model development; mouse genetics; mouse model; multi-electrode arrays; multidisciplinary; multiphoton imaging; neural circuit; neurodevelopment; neuroimaging; novel; optogenetics; phenotypic biomarker; pre-clinical; relating to nervous system; spatiotemporal; translational neuroscience

Preclinical Core Abstract The Preclinical Core (PC) provides IDDRC investigators with innovative, advanced approaches to conduct research spanning fine-grained resolution at the molecular and cellular level, to regional neurocircuitry and connectivity across brain, to multi-domain characterization of mouse behavior. In this application, 40 projects from 21 IDDRC investigators are proposed for core access in studies using models of ADHD, Angelman syndrome, ASD, childhood ataxia, epilepsy, intellectual disability, Joubert syndrome, Pitt-Hopkins syndrome, Rett syndrome, and other neurodevelopmental disorders. The PC has three components, the Mouse Behavioral Phenotyping Laboratory, the Neuroscience Microscopy Facility, and the Small Animal Imaging Service, which, together, offer a broad range of resources and expertise for multidisciplinary IDD research. Services include consultation with expert core faculty, an extensive battery of mouse behavioral testing, training in cutting-edge microscopy methods, acquisition and analysis of quantitative MRI data, and access to state-of-the-art laboratory facilities. A critical goal for the Core is to facilitate projects integrating complementary approaches, such as microscopy analysis of cortical architecture and behavioral evaluation of cognitive phenotypes, to enhance understanding of underlying pathophysiology and structure-function relationships in neurodevelopmental disorders. Within the IDDRC, the PC partners with the Clinical Translational Core and Data Science Core around the use of machine-learning approaches and the establishment of automated data-processing pipelines for new methods development. Overall, the PC provides expertise and infrastructure that has become essential for our IDDRC research using preclinical models, with the overarching aim of supporting breakthrough, transformative science in diagnosis, prevention, and treatment of developmental disorders. 1

临床前核心摘要 临床前核心 (PC) 为 IDDRC 研究人员提供创新、先进的方法来进行 研究范围涵盖分子和细胞水平的细粒度分辨率、区域神经回路和 跨大脑的连接，到小鼠行为的多域表征。在此应用程序中，有 40 个项目 21 名 IDDRC 研究者被提议使用 ADHD、Angelman 模型进行研究的核心访问 综合征、自闭症谱系障碍、儿童共济失调、癫痫、智力障碍、朱伯特综合征、皮特-霍普金斯综合征、 雷特综合征和其他神经发育障碍。 PC由三个组件组成，鼠标 行为表型实验室、神经科学显微镜设施和小动物成像 服务，共同为多学科 IDD 研究提供广泛的资源和专业知识。 服务包括与专家核心教师的咨询、广泛的小鼠行为测试、 尖端显微镜方法、定量 MRI 数据采集和分析以及获取 最先进的实验室设施。核心的一个关键目标是促进项目集成 补充方法，例如皮层结构的显微镜分析和行为评估 认知表型，以增强对潜在病理生理学和结构功能的理解 神经发育障碍中的关系。在 IDDRC 内，PC 与临床部门合作 围绕机器学习方法和技术的使用的转化核心和数据科学核心 为新方法开发建立自动化数据处理管道。总体而言，PC 提供专业知识和基础设施，这对于我们使用临床前的 IDDRC 研究至关重要 模型，其总体目标是支持诊断、预防、 和发育障碍的治疗。 1