CORE 2: Technology Core

核心2：技术核心

基本信息

批准号：
10224015
负责人：
Alexander Marson
金额：
$ 59.72万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-08-17 至 2023-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10224015
关键词：
Affinity Chromatography Bacterial Infections Bacterial Proteins Bioinformatics Biological Assay CRISPR/Cas technology Cell Line Cells Chlamydia trachomatis Clinical Clustered Regularly Interspaced Short Palindromic Repeats Collaborations Communities Complement Complex Core Facility Cryoelectron Microscopy Crystallography Data Disease Engineering Genes Genetic Genetic Epistasis Genetic Structures Genome Genome engineering Group Structure Immune Individual Infection Innate Immune Response Institutes Integration Host Factors Knock-out Laboratories Mass Spectrum Analysis Measures Methods Microscopy Mission Modeling Molecular Molecular Structure Mycobacterium tuberculosis Pathway Analysis Pathway interactions Phosphorylation Phylogenetic Analysis Post-Translational Protein Processing Proteins Proteome Proteomics Protocols documentation Reproducibility Research Personnel Resolution Resources Services Signal Transduction Signaling Protein Source Staphylococcus aureus Structural Protein Structure Techniques Technology Time Training and Education Transgenic Mice Translating Ubiquitination Work X-Ray Crystallography cost efficient crosslink data management embryonic stem cell experimental study functional genomics human pathogen innovation innovative technologies macrophage mass spectrometer mouse model neutrophil pathogen pathogenic bacteria protein complex protein protein interaction repaired response reverse genetics structural biology

项目摘要

CORE 2: TECHNOLOGY CORE SUMMARY The objective of the Technology Core is to provide cutting-edge and innovative technologies for the functional characterization of the genome and proteome in a reliable, reproducible, and cost-efficient manner. It will support Project 1 and Project 2 by facilitating proteomics, genetic interaction, and structural protein characterization experiments. In particular, the Technology Core will perform mass spectrometry characterization of host-pathogen protein-protein interactions (PPIs) and infection induced signaling network rewiring, CRISPR gene editing, and structural characterization by x-ray crystallography, cryo-electron microscopy (cryo-EM), and cross-link mass spectrometry (XL-MS). This core will be comprised of three well established facilities: the Thermo Fisher Scientific Proteomics Facility for Disease Target Discovery located at the J. David Gladstone Institutes, the UCSF Molecular Structures Group core facility located at the Mission Bay Campus of UCSF, and the Keck Advanced Microscopy Laboratory located at the Mission Bay Campus of UCSF. Additionally, we propose to create a new Functional Genomics Core (FGC) that leverages the Marson and Krogan lab’s expertise in genetic interaction mapping and genome engineering. The Technology Core will be led by pioneers in their respective fields, and who will employ state-of-the-art techniques, many of which were developed or optimized in their respective labs. The technologies described here will be used extensively by Project 1 and Project 2 investigators to accomplish the individual aims of this proposal, and will also serve as a resource to the wider scientific community by disseminating new assays and protocols.

核心2：技术核心概括技术核心的目的是为功能性提供最先进和创新的技术基因组和蛋白质组以可靠，可再现和成本效益的方式表征。会支持项目1和项目2通过支持蛋白质组学，遗传相互作用和结构蛋白表征实验。特别是，技术核心将执行质谱法宿主 - 病原体蛋白 - 蛋白质相互作用（PPI）和感染诱导信号网络的表征通过X射线晶体学，冷冻电子的重新布线，CRISPR基因编辑和结构表征显微镜（冷冻EM）和交联质谱法（XL-MS）。该核心将完成三井已建立的设施：疾病靶标的疾病的热渔民科学蛋白质组学设施 J. David Gladstone Institutes，位于Mission Bay的UCSF分子结构集团核心设施 UCSF的校园和位于Mission Bay校园的Keck Advanced显微镜实验室 UCSF。此外，我们建议创建一个利用MARSON的新功能基因组核心（FGC）克罗根实验室（Krogan Lab）在遗传相互作用映射和基因组工程方面的专业知识。技术核心将由各自领域的开拓者领导，谁将员工最先进技术，其中许多是在其各自的实验室中开发或优化的。描述的技术项目1和项目2调查人员将广泛使用此处的个人目标提案，还将通过传播新测定和协议。