Cell & Molecular Imaging Core

项目摘要 URMC IDDRC细胞和分子成像核心（CMI核心）为IDDRC成员提供 获得先进的成像、分析和基于病毒载体的转导方法，以及 相应的教师专业知识，以支持在体外和体内现象的研究， 亚细胞、细胞、组织和整个动物规模。所提供的能力是 IDDRC调查人员进行的研究，他们一直在使用现有的技术， 研究亚细胞，细胞，和系统水平的过程中发育的大脑的动物模型 还有人类这些尖端的方法与专家教师的指导相结合，确保 并简化对缺碘症研究至关重要的技术和设备的获取。 最先进的成像设备和软件提供了一系列图像采集和 分析方法（共聚焦，多光子和超分辨率成像）和我们的病毒载体 设施设计和生产定制的病毒方法，用于标记和操作 用于体外和体内成像和分析的信号传导途径。CMI核心由以下方面支持： 专门的行政和技术教师和工作人员提供定制的，关键的技术 在设计，实施和分析涉及显微镜的研究， IDDRC教师的利益。CMI核心利用现有的专家教师和工作人员， 长期的基础设施（共聚焦、多光子、分析），具有良好的使用记录 由IDDRC调查人员，与更新的能力（超分辨率，病毒载体），这已经被 根据公认的需求实施。结果是一个CMI核心，允许IDDRC 研究人员使用尖端技术设计和实施有意义的实验 和有效的分析范式，以解决困难和多样化的生物学问题。

Project Summary The URMC IDDRC Cell and Molecular Imaging Core (CMI Core) provides IDDRC members with access to advanced imaging, analysis, and viral vector-based transduction methods, as well as corresponding faculty expertise, to support in vitro and in vivo study of phenomena at the subcellular, cellular, tissue, and whole animal scale. The capabilities provided are central to research conducted by IDDRC investigators who have been using the available techniques to study subcellular, cellular, and systems level processes in the developing brain of animal models and humans. The integration of these cutting edge methods with expert faculty guidance ensures and streamlines access to techniques and equipment that are fundamental to IDD research. State-of-the-art imaging equipment and software provides a spectrum of image acquisition and analysis approaches (confocal, multiphoton and super-resolution imaging) and our viral vector facility designs and produces custom viral approaches for both labeling and manipulating signaling pathways for in vitro and in vivo imaging and analysis. The CMI Core is supported by dedicated administrative and technical faculty and staff to provide customized, critical technical expertise in the design, implementation, and analysis of studies involving microscopy that span the interests of the IDDRC faculty. The CMI Core leverages existing expert faculty and staff and long-standing infrastructure (confocal, multiphoton, analysis), with a proven track record of usage by IDDRC investigators, with newer capabilities (super-resolution, viral vectors), which have been implemented based upon recognized needs. The result is a CMI Core that allows IDDRC investigators to design and implement meaningful experiments using cutting edge technologies and efficient analysis paradigms to address difficult and diverse biological problems.