Cell & Molecular Imaging Core

细胞

• 批准号: 10445285
• 负责人: Anna K Majewska
• 金额: $ 18.5万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10445285
• 关键词: Address; Algorithms; Animal Model; Animals; Biological; Biological Process; Biometry; Brain; Clinical Sciences; Collaborations; Communities; Complex Analysis; Computer software; Computers; Consultations; Custom; Data; Data Analyses; Data Collection; Data Set; Ensure; Equipment; Experimental Designs; Facility Designs; Faculty; Human; Image; Image Analysis; Imaging Device; In Vitro; Individual; Infrastructure; Institutes; Intellectual and Developmental Disabilities Research Centers; Label; Laboratories; Laboratory Research; Methodology; Methods; Microscopy; Modification; Performance; Phase; Play; Process; Protocols documentation; Reproducibility; Research; Research Personnel; Research Project Grants; Resolution; Resources; Role; Sampling; Scientist; Signal Pathway; Standardization; System; Technical Expertise; Techniques; Technology; Time; Tissues; Training; Translational Research; Universities; Viral; Viral Vector; base; cellular imaging; data acquisition; data management; data sharing; design; experience; experimental study; image processing; imaging modality; improved; in vivo; in vivo imaging; instrumentation; interest; member; molecular imaging; programs; spectrograph; success; theories; tool

Project Summary The URMC IDDRC Cell and Molecular Imaging Core (CMI Core) provides IDDRC members with access to advanced imaging, analysis, and viral vector-based transduction methods, as well as corresponding faculty expertise, to support in vitro and in vivo study of phenomena at the subcellular, cellular, tissue, and whole animal scale. The capabilities provided are central to research conducted by IDDRC investigators who have been using the available techniques to study subcellular, cellular, and systems level processes in the developing brain of animal models and humans. The integration of these cutting edge methods with expert faculty guidance ensures and streamlines access to techniques and equipment that are fundamental to IDD research. State-of-the-art imaging equipment and software provides a spectrum of image acquisition and analysis approaches (confocal, multiphoton and super-resolution imaging) and our viral vector facility designs and produces custom viral approaches for both labeling and manipulating signaling pathways for in vitro and in vivo imaging and analysis. The CMI Core is supported by dedicated administrative and technical faculty and staff to provide customized, critical technical expertise in the design, implementation, and analysis of studies involving microscopy that span the interests of the IDDRC faculty. The CMI Core leverages existing expert faculty and staff and long-standing infrastructure (confocal, multiphoton, analysis), with a proven track record of usage by IDDRC investigators, with newer capabilities (super-resolution, viral vectors), which have been implemented based upon recognized needs. The result is a CMI Core that allows IDDRC investigators to design and implement meaningful experiments using cutting edge technologies and efficient analysis paradigms to address difficult and diverse biological problems.

项目摘要 URMC IDDRC细胞和分子成像核心(CMI Core)为IDDRC成员提供 获得先进的成像、分析和基于病毒载体的转导方法，以及 相应的教师专业知识，以支持在体外和体内的现象研究 亚细胞、细胞、组织和整个动物的尺度。所提供的功能是 IDDRC调查人员进行的研究，他们一直在使用可用的技术来 研究动物模型脑发育中的亚细胞、细胞和系统水平的过程 和人类。将这些尖端方法与专家教师指导相结合，确保 并简化对IDD研究至关重要的技术和设备的获取。 最先进的成像设备和软件提供了一系列图像采集和 分析方法(共聚焦、多光子和超分辨率成像)和我们的病毒载体 设施设计和生产定制病毒方法，用于标签和操作 用于体外和体内成像和分析的信号通路。CMI Core由支持 专职的行政和技术教职员工，提供定制的关键技术 在涉及显微镜研究的设计、实施和分析方面的专业知识 IDDRC教职员工的利益。CMI核心利用现有的专家教职员工和 长期的基础设施(共焦、多光子、分析)，具有久经考验的使用记录 由IDDRC调查人员开发，具有更新的能力(超分辨率、病毒载体)，已被 根据公认的需求实施。结果是一个允许IDDRC的CMI核心 研究人员使用尖端技术设计和实施有意义的实验 和有效的分析范式，以解决困难和多样化的生物学问题。