Core 1: Biospecimen and Pathology Core

核心 1：生物样本和病理学核心

• 批准号: 10226085
• 负责人: Dipen M. Maru
• 金额: $ 24.84万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-20 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10226085
• 关键词: Affect; Allografting; Apoptosis; Biological Assay; Biological Markers; Biopsy; Blood; Blood specimen; CLIA certified; Cancer Center; Carcinoma; Cellularity; Clinical Distribution; Clinical Trials; Closure by clamp; Collection; Colorectal; Colorectal Cancer; Conduct Clinical Trials; Core Biopsy; Cryopreservation; Custom; Data; Databases; Derivation procedure; Development; Diagnosis; Documentation; Endoscopic Biopsy; Enrollment; Ensure; Environment; Excision; Faculty; Familial Adenomatous Polyposis Syndrome; Formalin; Freezing; Funding; Grant; Guidelines; Hepatic; Histology; Histopathology; Human; Human Resources; IL6 gene; Image; Image Analysis; Immunohistochemistry; Immunotherapy; Informatics; Information Services; Information Systems; Infrastructure; Institutional Review Boards; Laboratories; Lesion; Malignant neoplasm of gastrointestinal tract; Malignant neoplasm of pancreas; Mucinous Neoplasm; Mus; Necrosis; Needles; Neoplasm Metastasis; Normal tissue morphology; Operative Surgical Procedures; Pancreas; Pancreatic Ductal Adenocarcinoma; Paraffin Embedding; Pathologist; Pathology; Pathology Report; Patient Care; Patients; Peer Review; Peptide Vaccines; Preventive; Process; Proliferation Marker; Protocols documentation; Quality Control; Reporting; Research; Research Personnel; Research Project Grants; Resected; Resources; STAT3 gene; Sampling; Services; Specimen; Stains; Surgical Pathology; System; Technical Expertise; Testing; Time; Tissue Banks; Tissue Microarray; Tissue Sample; Tissues; Tumor Tissue; Tumor-Infiltrating Lymphocytes; University of Texas M D Anderson Cancer Center; Validation; Xenograft procedure; adenoma; automated image analysis; base; career; clinical care; co-clinical trial; colorectal cancer prevention; design; gastrointestinal; inhibitor/antagonist; metastatic colorectal; molecular pathology; patient derived xenograft model; pre-clinical; predictive marker; programs; prospective; quantitative imaging; standard of care; tumor; vaccine evaluation

CORE 1 BIOSPECIMEN AND PATHOLOGY: Summary/Abstract The Biospecimen and Pathology Core will coordinate efforts related to collection, processing, storage and distribution of annotated human and murine biospecimens for all of the SPORE projects, including the Career Enhancement Program (CEP) and Developmental Research Program (DRP). The Core will be co-led by two nationally reputed gastrointestinal/pancreatic pathologists, Drs. Dipen Maru and Huamin Wang. For human biospecimens, the core will interface with the MDACC Institutional Tissue Bank (ITB). Biospecimen resources from the lower gastrointestinal tissue bank include freshly collected/snap frozen and formalin fixed paraffin embedded tumor and normal specimens from more than 2500 resected hepatic colorectal metastases (including TMAs), freshly collected and snap frozen adenoma from 334 patients and formalin fixed paraffin embedded specimens from 870 or more patients with sporadic adenoma or familial adenomatous polyposis. Existing biospecimen resources available in the pancreatic bank and related IRB approved protocols include freshly collected/snap frozen tumor and normal tissue sample from Whipple resection for pancreatic ductal adenocarcinoma from 232 patients, with formalin fixed paraffin embedded specimens and additional 672 patients, including tissue microarrays from pancreatic ductal adenocarcinoma and intraductal pancreatic mucinous neoplasms. The Core will support Project 1 by coordinating prospective blood collection, cryopreservation and transport with the ITB. Specifically, Core faculty will prospectively collect, process and distribute fresh tumor and normal samples from hepatic colorectal metastases after obtaining mirror image section for histology quality control. The Core will provide formalin fixed paraffin embedded samples of normal, adenoma and carcinoma to Project 2. In addition the Core will provide biospecimen qualification services, including but not limited to, histopathologic characterization of human and murine tissues treated with STAT3 inhibitor, and immunohistochemistry staining and interpretation, including validation of p-STAT3 staining by automated image analysis in a CLIA-certified facility. The Core will provide freshly resected PDAC samples for patient derived xenografts and ex vivo live tissue sensitivity assay (LTSA) for Project 3. In addition, the Core will provide histopathology characterization, immunohistochemistry services and interpretation guidelines for both preclinical samples from the ongoing co-clinical trials, as well as serial tissue biopsies obtained from the two clinical trials being conducted Project 3. The Core will also coordinate distribution of appropriate samples to investigators funded through the SPORE CEP and DRP grants. The Core personnel, along with the ITB, will enter detailed information related to all processes of biospecimen collection, processing, qualification, distribution and analytes extraction into the Institutional Biospecimen Informatics platform known as “Tissue Station”. The Core activities will lead to enhancement of these functionalities of Tissue Station and design a new interface specific for GI SPORE in the Tissue Station.

核心 1 生物标本和病理学：总结/摘要 生物样本和病理学核心将协调与收集、处理、储存和 为所有 SPORE 项目（包括 Career）分发带注释的人类和鼠类生物样本 增强计划（CEP）和发展研究计划（DRP）。核心将由两人共同领导 全国知名的胃肠/胰腺病理学家，博士。 Dipen Maru 和华民王。对于人类 生物样本，核心将与 MDACC 机构组织库 (ITB) 连接。生物样本资源 来自下胃肠道组织库，包括新鲜收集/速冻和福尔马林固定石蜡 来自 2500 多个切除的肝结直肠转移瘤（包括 TMA），来自 334 名患者的新鲜采集并速冻的腺瘤，福尔马林固定石蜡包埋 来自 870 名或更多散发性腺瘤或家族性腺瘤性息肉病患者的标本。现存的 胰腺库中可用的生物样本资源和相关 IRB 批准的方案包括新鲜的 从胰管 Whipple 切除术中收集/快照冷冻肿瘤和正常组织样本 来自 232 例患者的腺癌，福尔马林固定石蜡包埋标本和另外 672 例 患者，包括来自胰腺导管腺癌和导管内胰腺癌的组织微阵列 粘液性肿瘤。核心将通过协调未来的血液采集来支持项目 1， 使用 ITB 进行冷冻保存和运输。具体来说，核心教师将前瞻性地收集、处理和 获得镜像后分发来自肝结直肠转移瘤的新鲜肿瘤和正常样本 组织学质量控制部分。核心将提供福尔马林固定石蜡包埋的正常样本、 项目 2 的腺瘤和癌。此外，核心将提供生物样本鉴定服务， 包括但不限于用 STAT3 处理的人类和鼠组织的组织病理学特征 抑制剂、免疫组织化学染色和解释，包括 p-STAT3 染色的验证 在 CLIA 认证的设施中进行自动图像分析。 Core 将为以下患者提供新鲜切除的 PDAC 样本： 项目 3 的患者来源的异种移植物和离体活组织敏感性测定 (LTSA)。此外，核心将 为两者提供组织病理学表征、免疫组织化学服务和解释指南 来自正在进行的临床联合试验的临床前样本，以及从两个项目获得的连续组织活检 正在进行项目 3 的临床试验。核心还将协调适当样本的分配，以 研究人员通过 SPORE CEP 和 DRP 拨款资助。核心人员与 ITB 一起，将 输入与生物样本采集、处理、鉴定、分发所有过程相关的详细信息 并将分析物提取到被称为“组织站”的机构生物样本信息学平台中。这 核心活动将增强 Tissue Station 的这些功能并设计新界面 专用于组织站中的 GI SPORE。