Center for Advanced Microscopy Shared Resource

摘要--高级显微镜中心 高级显微镜中心(CAM)提供培训、访问、支持和专业知识，使Lurie能够 癌症中心(LCC)成员成功利用先进的显微技术和仪器 他们的研究。西北大学(NU)的调查人员大量使用它，有104名LCC成员使用 是去年的事了。我们的设施一周7天24小时开放，并提供通往美国多个州的通道 ART光学和电子显微镜(EM)仪器、辅助设备和试剂，允许成像 在一系列空间尺度上进行。我们不断扩大的乐器组合和 CAM工作人员的专业知识使用户能够成像单分子、大分子网络、活细胞、组织 切片、整个器官和模式生物的亮场、荧光和发光。我们支持 先进的成像技术，如FRET、FRAP、FLIP、TIRF、光转换和免疫金以及 白金复制品EM。该机构积极寻求资金，并取得了很大成功。 各种来源，以保持LCC在新型成像技术方面的领先地位。例如，CAM是 在美国只有三个尼康成像中心，允许我们的用户测试来自 尼康。CAM还提供工作站、一套软件和专家支持，包括最近租用的Image 分析专家，以促进图像数据的传输、存储、管理和分析。该设施还 提供广泛的教育项目，包括课程、研讨会、杂志俱乐部和实践 研讨会。

ABSTRACT – CENTER FOR ADVANCED MICROSCOPY The Center for Advanced Microscopy (CAM) provides training, access, support and expertise that enable Lurie Cancer Center (LCC) members to successfully utilize advanced microscopy techniques and instrumentation in their studies. It is heavily used by Northwestern University (NU) investigators, with 104 LCC members using the facility last year. Our facility is open 24 hours/7 days a week and provides access to a wide array of state of the art light and electron microscopy (EM) instrumentation, auxiliary equipment, and reagents, permitting imaging to be performed across a range of spatial scales. Our continuously expanding portfolio of instruments and the expertise of CAM staff enable users to image single molecules, macromolecular networks, live cells, tissue sections, whole organs and model organisms with brightfield, fluorescence and luminescence. We support advanced imaging techniques such as FRET, FRAP, FLIP, TIRF, photo-conversion, and immuno-gold and platinum replica EM. The facility has aggressively, and with a high degree of success, pursued funding from a variety of sources to maintain the LCC’s leadership in novel imaging technologies. For example, CAM is one of only three Nikon Imaging Centers in the United States, allowing our users to test the latest technologies from Nikon. CAM also provides workstations, a suite of software, and expert support, including a recently hired Image Analysis Specialist, to facilitate imaging data transfer, storage, management and analysis. The facility also provides a wide array of educational programs, including courses, seminars, journal clubs, and hands-on workshops.