Center for Advanced Microscopy Shared Resource

高级显微镜共享资源中心

• 批准号: 10902176
• 负责人: Constadina Arvanitis
• 金额: $ 15.87万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-15 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10902176
• 关键词: 3-Dimensional; Applications Grants; Award; Calcium; Cancer Biology; Cancer Center; Cells; Comprehensive Cancer Center; Computer software; Conceptions; Confocal Microscopy; Core Facility; Data; Data Analyses; Data Storage and Retrieval; Decentralization; Dedications; Education; Education and Outreach; Educational workshop; Electron Microscope; Electron Microscopy; Equipment; Fluorescence; Fluorescence Resonance Energy Transfer; Funding; Hour; Image; Image Analysis; Imaging Techniques; Imaging technology; Investigation; Journals; Leadership; Light; Manuscripts; Microscope; Microscopy; Organ; Phase; Platinum; Plug-in; Preparation; Reagent; Research; Research Personnel; Resource Sharing; Resources; Scanning; Secure; Services; Slice; Source; Specialist; System; Techniques; Technology; Testing; Tissue imaging; Tissues; Training; United States; Universities; Whole Organism; base; cell fixing; data exchange; data management; imaging approach; imaging facilities; imaging system; improved; instrument; instrumentation; light microscopy; live cell imaging; luminescence; member; model organism; novel imaging technology; programs; routine imaging; single molecule; success; technology platform; ultra high resolution

ABSTRACT – CENTER FOR ADVANCED MICROSCOPY The Center for Advanced Microscopy (CAM) provides training, access, support and expertise that enable Lurie Cancer Center (LCC) members to successfully utilize advanced microscopy techniques and instrumentation in their studies. It is heavily used by Northwestern University (NU) investigators, with 104 LCC members using the facility last year. Our facility is open 24 hours/7 days a week and provides access to a wide array of state of the art light and electron microscopy (EM) instrumentation, auxiliary equipment, and reagents, permitting imaging to be performed across a range of spatial scales. Our continuously expanding portfolio of instruments and the expertise of CAM staff enable users to image single molecules, macromolecular networks, live cells, tissue sections, whole organs and model organisms with brightfield, fluorescence and luminescence. We support advanced imaging techniques such as FRET, FRAP, FLIP, TIRF, photo-conversion, and immuno-gold and platinum replica EM. The facility has aggressively, and with a high degree of success, pursued funding from a variety of sources to maintain the LCC’s leadership in novel imaging technologies. For example, CAM is one of only three Nikon Imaging Centers in the United States, allowing our users to test the latest technologies from Nikon. CAM also provides workstations, a suite of software, and expert support, including a recently hired Image Analysis Specialist, to facilitate imaging data transfer, storage, management and analysis. The facility also provides a wide array of educational programs, including courses, seminars, journal clubs, and hands-on workshops.

摘要 - 高级显微镜中心 高级显微镜中心（CAM）提供培训，访问，支持和专业知识，使Lurie能够 癌症中心（LCC）成员成功利用高级显微镜技术和仪器 他们的研究。它被西北大学（NU）的调查员大量使用，有104位LCC成员使用 去年的设施。我们的设施每周24小时/7天开放，可访问各种状态 艺术光和电子显微镜（EM）仪器，辅助设备和试剂，允许成像为 在一系列空间尺度上进行。我们不断扩展的乐器投资组合和 CAM员工的专业知识使用户能够成像单分子，大分子网络，活细胞，组织 截面，整个器官和模型的生物体，具有明亮的荧光，荧光和发光。我们支持 高级成像技术，例如FRET，FRAP，FLIP，TIRF，PHOTOCREVERSION和IMMUNO-GOLD，以及 白金复制品。该设施具有积极的成功，并取得了很大的成功，从 多种来源，以保持LCC在新型成像技术中的领导能力。例如，凸轮是 美国只有三个尼康成像中心，使我们的用户可以测试来自 尼康。 CAM还提供工作站，一套软件和专家支持，包括最近雇用的图像 分析专家，托管成像数据传输，存储，管理和分析。设施也是如此 提供各种各样的教育计划，包括课程，半岛，期刊俱乐部和动手 讲习班。