DCEG- Molecular Assays for epidemiology studies

DCEG-流行病学研究的分子测定

• 批准号: 10273216
• 负责人: LEONARD FREEDMAN
• 金额: $ 302.89万
• 依托单位: LEIDOS BIOMEDICAL RESEARCH, INC.
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-31 至 2021-08-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10273216
• 关键词: Biological; Biological Assay; Biological Process; Blood specimen; CLIA certified; Cell Line; Cells; Cervical; Clinical Research; Contracts; Cryopreservation; Cryopreserved Cell; Cytology; DNA; Detection; Diagnostics Research; Division of Cancer Epidemiology and Genetics; Epidemiology; Erythrocytes; Feces; Fibroblasts; Flow Cytometry; Fluorescence-Activated Cell Sorting; Freeze Drying; Freezing; Hormones; Human; Human Herpesvirus 4; Immunologic Monitoring; Immunologic Tests; Immunologics; In Vitro; Infrastructure; Laboratories; Liquid substance; Malignant neoplasm of cervix uteri; Mass Spectrum Analysis; Measurement; Molecular; Molecular Epidemiology; Mutation Detection; Nitrogen; Nucleic Acids; Paraffin Embedding; Pathogen detection; Pathogenesis; Peripheral Blood Mononuclear Cell; Phenotype; Plasma; Polymorphism Analysis; Preparation; Prevention; Process; Proteins; Quality Control; RNA; Reporting; Research Personnel; Scientist; Serum; Services; Single Nucleotide Polymorphism; Specimen; Specimen Handling; Spectrophotometry; Structure; Techniques; Technology; Tissue Embedding; Tissues; Urine; Virus; Whole Blood; anticancer research; biobank; bioprocess; blood fractionation; cancer epidemiology; cell immortalization; cold temperature; epidemiology study; established cell line; instrumentation; molecular diagnostics; programs; quality assurance; repository; small molecule; tumor

Activities performed within this project provide DNA extraction, alliquoting, bioprocessing of human epidemiology specimens for cancer research. Several facilities are utilized to process human specimens and to provide the necessary infrastructure to support human specimen processing and storage in support of epidemiology cancer research. The Mass Spectrometry Center is equipped with the latest in mass spectrometry (MS) instrumentation for analyzing both small and large protein biomolecules, including endogenous hormones. The Center offers corresponding expertise in detection, quantitation, structure confirmation, and mass measurements. The Center also educates scientists on the use, interpretation, and applications of MS technologies. The CLIA-certified Molecular Diagnostics Laboratory provides state-of-the-art clinical and research diagnostics, including mutation detection, single nucleotide polymorphism analysis, and pathogen detection. The Human Immunological Monitoring Laboratory supports cervical cancer research by examining human host immunological parameters potentially important in the pathogenesis or prevention of virus-related tumors. The laboratory's capabilities include: receipt and processing of human specimens, including whole blood and cervical specimens; cryopreservation, DNA extraction and Epstein-Barr virus transformation of peripheral blood mononuclear cells (PBMC); immunological testing, including a variety of in vitro PBMC functional assays; HLA and PBMC phenotyping by flow cytometry (fluorescence activated cell sorting – FACS); temporary low temperature storage of biological specimens and transfer to the DCEG biorepository; electronic tracking of biospecimens and reporting of assay results to DCEG investigators; maintain quality control/quality assurance programs; apply state-of-the-art techniques, as they become available. The Bioprocessing Laboratory processes biological specimens prior to their storage in the DCEG repository or dispersal to collaborators. The processing laboratory provides: processing and freezing of biological specimens, including whole blood, serum, plasma, urine, and feces; lyophilization of specimens; flash-freezing of tissue in liquid nitrogen; separation and viable cryopreservation of cell lines, including the routine assessment of viability; cryopreservation of red blood cells to maintain enzymatic activity; establishment of cell lines; large scale propagation of cell lines; nucleic acid extraction; distribution of specimens. The DNA Extraction Laboratory, a part of the CGR, extracts DNA from whole blood specimens, blood fractions, transformed lymphoblastic lines, cultured fibroblasts, tumor specimens, buccal cells, paraffin-embedded tissue, Guthrie cards, cytology preparations, or other biological specimens. Activities performed within this project provide DNA extraction, alliquoting, bioprocessing of human epidemiology specimens for cancer research.

在该项目范围内开展的活动包括DNA提取、等分、癌症研究人类流行病学标本的生物处理。几个设施被用来处理人类标本，并提供必要的基础设施，以支持人类标本的处理和储存，以支持流行病学癌症研究。质谱中心配备了最新的质谱（MS）仪器，用于分析小型和大型蛋白质生物分子，包括内源性激素。该中心在检测、定量、结构确认和质量测量方面提供相应的专业知识。该中心还教育科学家使用，解释和MS技术的应用。CLIA认证的分子诊断实验室提供最先进的临床和研究诊断，包括突变检测，单核苷酸多态性分析和病原体检测。人类免疫学监测实验室通过检查人类宿主免疫学参数来支持宫颈癌研究，这些参数在病毒相关肿瘤的发病机制或预防中可能很重要。该实验室的能力包括：接收和处理人体标本，包括全血和宫颈标本;外周血单核细胞（PBMC）的冷冻保存、DNA提取和EB病毒转化;免疫学测试，包括各种体外PBMC功能测定;通过流式细胞术进行HLA和PBMC表型分析（荧光激活细胞分选- FACS）;暂时低温储存生物样本并转移至DCEG生物储存库;电子跟踪生物样本并向DCEG研究者报告测定结果;保持质量控制/质量保证计划;应用最先进的技术，因为它们变得可用。生物处理实验室在生物标本储存在DCEG储存库或分发给合作者之前对其进行处理。处理实验室提供：生物标本的处理和冷冻，包括全血、血清、血浆、尿液和粪便;标本的冻干;组织在液氮中的快速冷冻;细胞系的分离和活性冷冻保存，包括活性的常规评估;红细胞的冷冻保存以保持酶活性;细胞系的建立;细胞系的大规模繁殖;核酸提取;标本分配。DNA提取实验室是CGR的一部分，从全血标本、血液组分、转化的淋巴母细胞系、培养的成纤维细胞、肿瘤标本、颊细胞、石蜡包埋组织、古特里卡、细胞学制剂或其他生物标本中提取DNA。在该项目范围内开展的活动包括DNA提取、等分、癌症研究人类流行病学标本的生物处理。