Proteomic and Functional Analysis of Presynaptic Physiology and Plasticity

摘要 突触前神经元可塑性的分子机制及其在学习和行为中的作用， 记忆仍然知之甚少，主要是由于缺乏对记忆信号分子的了解。 突触前细胞基质和工具，空间和时间操纵突触前可塑性。中的这一空白 知识是进入该领域的根本障碍。在这个项目中，我们将开发和利用创新的蛋白质组学， 基因组编辑和光遗传学方法来解决这些问题，揭示蛋白质和内部工作原理， 不同神经元细胞类型的突触前细胞基质及其在学习和记忆中的作用。我们 预期这些数据将为未来突触前神经元的研究提供一个新的和无与伦比的分子框架。 生理学以及对突触前可塑性如何调节行为的见解。

ABSTRACT The molecular mechanisms of plasticity within the presynapse and its role in behaviors such as learning and memory are still poorly understood, mainly due to a lack of knowledge of the signaling molecules of the presynaptic cytomatrix and tools to spatially and temporally manipulate presynaptic plasticity. This gap in knowledge is a fundamental barrier to the field. In this project, we will develop and utilize innovative proteomic, genome editing, and optogenetic approaches to solve these problems, revealing the proteins and inner workings of the cytomatrix of presynapses from distinct neuronal cell types and their roles in learning and memory. We anticipate these data will provide a new and unparalleled molecular framework for future studies on presynaptic physiology as well as insights into how forms of presynaptic plasticity modulate behavior.