DNA CHARACTERIZATION SERVICES FOR CCG, CRS/EOMI, CPTAC AND DCEG TASK ORDERTO PERIOD OF PERFORMANCE: 08/31/2019 - 08/30/2021 OPTION 1 PERIOD OF PERFO

Broad研究所将建立一个基因组表征中心（GCC）来支持癌症基因组学管道中心，我们建议在我们30年的基因组中心记录的基础上，应用一套经过验证的平台和先进的分析工具来全面表征癌症标本的基因组变化。我们将描述每年至少1200例由NCI癌症基因组学中心确定的癌症病例。我们将应用基于dna的分析，包括低覆盖率全基因组测序（WGS），高覆盖率WGS，全外显子组（WEX），靶向面板和基于甲基化的分析来识别各种类型的体细胞改变。我们能够接受并成功地分析来自FFPE样品的材料，并记录了这种类型样品的丰富经验。

The Broad Institute will establish a Genome Characterization Center (GCC) to support the Center for Cancer Genomics Pipeline, we propose to build on our 30 year track record as a Genome Center and apply a suite of well-validated platforms and advanced analytic tools to comprehensively characterize genomic alterations of cancer specimens. We will characterize at least 1,200 cancer cases per year as identified by the NCI Center for Cancer Genomics. We will apply DNA-based assays including low coverage whole genome sequencing (WGS), high coverage WGS, whole exome (WEX), targeted panel and methylation-based assays to identify a wide array of types of somatic alterations. We are able to accept and successfully analyze materials from FFPE specimens and have documented extensive experience with this type of sample.