Request for an Olympus VS200 slide scanner

索取奥林巴斯 VS200 玻片扫描仪

• 批准号: 10280468
• 负责人: KENNETH N FISH
• 金额: $ 37.04万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2022-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10280468
• 关键词: 3-Dimensional; Anatomy; Area; Biological; Brain; Brain region; Communities; Core Facility; Data; Devices; Electrons; Eye; Frustration; Funding; Genes; Home; Image; Image Analysis; Institution; Light; Location; Medical; Medical Research; Methods; Microscope; Modality; Molecular; National Institute of Mental Health; Neck; Neuraxis; Oils; Research; Research Personnel; Research Project Grants; Resolution; Scanning; Science; Slide; System; Time; Universities; Work; base; image guided; imaging system; instrument; medical schools; operation; optical imaging; protein distribution; protein expression

This request is for funds to purchase an Olympus VS200 slide scanner with a multislide feeder and a storage server to hold data generated by the system. This instrument is being requested by a group of NIMH funded investigators who have a significant need to scan many hundreds of slides at high resolution in both brightfield and fluorescent modalities. The device will be housed within the Center for Biologic Imaging (CBI) at the University of Pittsburgh School of Medicine. The mandate of this core facility is to provide access to a full range of light and electron optical, image analysis, and morphometric methods to all research groups within the University of Pittsburgh School of Medicine. To gain a full and quantitative appreciation of the distribution of proteins and active genes within the central nervous system it is no longer sufficient to scan by eye and collect snapshot images that guide science as this commonly introduces bias and is certainly not appropriate when protein expression is at low levels or expressed in very discrete locations throughout brain regions. Rather there is a pressing and prevalent need to analyze distributions in 3D, commonly over many sections and over large areas. The investigators in this proposal have been using the “home brew” solutions available within the CBI. While these devices may have the spatial and spectral sensitivity to meet the needs of the investigative team they need to be tended to throughout the imaging to select image regions, add oil or change slides and are also being used by the very large number of investigators who are actively working with the CBI systems. Therefore, there is a single prime reason for this application: to provide critically needed high quality, automated instrument time to the NIMH supported users of the Center for Biologic Imaging. Since the CBI commenced operations 28 years ago, it has become an integral part of the medical research community currently participating in research projects with more than 300 PHS funded groups within the medical area, as well as in PHS supported projects with investigators in other departments and at neighboring institutions. The NIMH funded user base within the institution continues to grow and its requirement for large area automated imaging continues to expand. The lack of available scanning time has become a major limiting factor in the utility of the CBI to this specific group of users which has led to considerable frustration, as progress in funded work is absolutely dependent on high quality quantitative molecular anatomy of brain sections. The acquisition of an automated slide scanning microscope specifically for this user group will be a tremendous addition to the activity and functionality of the center as it will alleviate access on pre-existing systems for other user groups and will help alleviate the current user bottle neck for high resolution slide scanning by our NIMH funded colleagues.

此请求用于购买带有多玻片进纸器的 Olympus VS200 玻片扫描仪的资金 以及存储服务器来保存系统生成的数据。一个团体正在请求该仪器 NIMH 资助的研究人员非常需要高速扫描数百张载玻片 明场和荧光模式下的分辨率。该设备将安置在中心内 匹兹堡大学医学院的生物成像 (CBI)。该核心设施的任务 是提供全方位的光和电子光学、图像分析和形​​态测量 匹兹堡大学医学院所有研究小组的方法。 全面、定量地了解蛋白质和活性基因的分布 对于中枢神经系统来说，仅仅通过眼睛扫描并收集指导的快照图像已经不够了 科学，因为这通常会引入偏差，并且当蛋白质表达处于低水平时当然是不合适的 低水平或在整个大脑区域的非常离散的位置表达。反而有一种紧迫感 普遍需要分析 3D 分布，通常是在许多部分和大面积上。 该提案中的调查人员一直在使用 CBI 内提供的“自制”解决方案。 虽然这些设备可能具有空间和光谱灵敏度来满足调查的需要 他们需要在整个成像过程中照顾团队，以选择图像区域、添加油或更换载玻片 并且也被大量积极与 CBI 合作的调查人员使用 系统。因此，此应用程序有一个主要原因：提供急需的高 向 NIMH 支持的生物成像中心用户提供优质、自动化的仪器时间。 CBI自28年前开始运作以来，已成为医疗领域不可或缺的一部分。 研究界目前正在与 300 多个 PHS 资助的团体一起参与研究项目 在医疗领域以及 PHS 支持的项目中与其他部门的研究人员和 在邻近的机构。 NIMH 资助的机构内用户群不断增长，其对大型 自动化成像领域不断扩大。缺乏可用的扫描时间已成为一个主要问题 CBI 对这一特定用户群的效用的限制因素，这导致了相当大的 令人沮丧的是，资助工作的进展绝对依赖于高质量的定量分子 大脑部分的解剖学。购置一台自动载玻片扫描显微镜，专门用于 该用户组将极大地丰富该中心的活动和功能，因为它将减轻 访问其他用户组的现有系统，将有助于缓解当前的用户瓶颈 由 NIMH 资助的同事进行高分辨率玻片扫描。