Request for an Olympus VS200 slide scanner
索取奥林巴斯 VS200 玻片扫描仪
基本信息
- 批准号:10280468
- 负责人:
- 金额:$ 37.04万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-08-01 至 2022-07-31
- 项目状态:已结题
- 来源:
- 关键词:3-DimensionalAnatomyAreaBiologicalBrainBrain regionCommunitiesCore FacilityDataDevicesElectronsEyeFrustrationFundingGenesHomeImageImage AnalysisInstitutionLightLocationMedicalMedical ResearchMethodsMicroscopeModalityMolecularNational Institute of Mental HealthNeckNeuraxisOilsResearchResearch PersonnelResearch Project GrantsResolutionScanningScienceSlideSystemTimeUniversitiesWorkbaseimage guidedimaging systeminstrumentmedical schoolsoperationoptical imagingprotein distributionprotein expression
项目摘要
This request is for funds to purchase an Olympus VS200 slide scanner with a multislide feeder
and a storage server to hold data generated by the system. This instrument is being requested by a group
of NIMH funded investigators who have a significant need to scan many hundreds of slides at high
resolution in both brightfield and fluorescent modalities. The device will be housed within the Center for
Biologic Imaging (CBI) at the University of Pittsburgh School of Medicine. The mandate of this core facility
is to provide access to a full range of light and electron optical, image analysis, and morphometric
methods to all research groups within the University of Pittsburgh School of Medicine.
To gain a full and quantitative appreciation of the distribution of proteins and active genes within
the central nervous system it is no longer sufficient to scan by eye and collect snapshot images that guide
science as this commonly introduces bias and is certainly not appropriate when protein expression is at
low levels or expressed in very discrete locations throughout brain regions. Rather there is a pressing
and prevalent need to analyze distributions in 3D, commonly over many sections and over large areas.
The investigators in this proposal have been using the “home brew” solutions available within the CBI.
While these devices may have the spatial and spectral sensitivity to meet the needs of the investigative
team they need to be tended to throughout the imaging to select image regions, add oil or change slides
and are also being used by the very large number of investigators who are actively working with the CBI
systems. Therefore, there is a single prime reason for this application: to provide critically needed high
quality, automated instrument time to the NIMH supported users of the Center for Biologic Imaging.
Since the CBI commenced operations 28 years ago, it has become an integral part of the medical
research community currently participating in research projects with more than 300 PHS funded groups
within the medical area, as well as in PHS supported projects with investigators in other departments and
at neighboring institutions.
The NIMH funded user base within the institution continues to grow and its requirement for large
area automated imaging continues to expand. The lack of available scanning time has become a major
limiting factor in the utility of the CBI to this specific group of users which has led to considerable
frustration, as progress in funded work is absolutely dependent on high quality quantitative molecular
anatomy of brain sections. The acquisition of an automated slide scanning microscope specifically for
this user group will be a tremendous addition to the activity and functionality of the center as it will alleviate
access on pre-existing systems for other user groups and will help alleviate the current user bottle neck
for high resolution slide scanning by our NIMH funded colleagues.
此申请是为购买Olympus VS200载玻片扫描仪(带多玻片进样器)提供资金
以及存储服务器,用于保存由所述系统生成的数据。一个团体要求使用这一工具,
的NIMH资助的研究人员有显着需要扫描数百个幻灯片在高
在明场和荧光模式下的分辨率。该器械将存放在中心内,
匹兹堡大学医学院的生物成像(CBI)。这一核心融资机制的任务
是提供全方位的光和电子光学,图像分析和形态测量
匹兹堡大学医学院的所有研究小组。
为了获得蛋白质和活性基因分布的全面和定量的评价,
中枢神经系统,它不再是足够的扫描眼睛和收集快照图像,引导
科学,因为这通常会引入偏见,当然是不合适的,当蛋白质表达是在
低水平或在整个大脑区域非常离散的位置表达。相反,有一个紧迫的
并且普遍需要在3D中分析分布,通常在许多截面和大面积上。
这项提案中的调查人员一直在使用CBI内部可用的“自制”解决方案。
虽然这些设备可以具有空间和光谱灵敏度以满足调查的需要,
团队需要在整个成像过程中对其进行护理,以选择图像区域、添加润滑油或更换载玻片
也被大量积极与CBI合作的调查人员使用
系统.因此,这种应用有一个主要原因:提供急需的高
质量,自动化仪器时间NIMH支持的生物成像中心的用户。
自28年前开始运作以来,CBI已成为医疗保健的一个组成部分。
目前参与研究项目的研究团体有300多个PHS资助团体
在医疗领域内,以及在PHS支持的项目与其他部门的调查人员,
在邻近的机构。
NIMH资助的用户群在机构内继续增长,其对大型
区域自动成像不断扩展。缺乏可用的扫描时间已成为一个主要的问题,
CBI对这一特定用户群体的效用的限制因素,这导致了相当大的
挫折,因为资助工作的进展绝对依赖于高质量的定量分子
脑切片的解剖学购买一台自动载玻片扫描显微镜,
这一用户群将极大地增加中心的活动和功能,因为它将减轻
为其他用户组提供对现有系统的访问,这将有助于缓解当前的用户瓶颈
由NIMH资助的同事进行高分辨率幻灯片扫描。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
KENNETH N FISH其他文献
KENNETH N FISH的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('KENNETH N FISH', 18)}}的其他基金
Deciphering the GABA neuron alterations in schizophrenia
破译精神分裂症中 GABA 神经元的改变
- 批准号:
9761684 - 财政年份:2019
- 资助金额:
$ 37.04万 - 项目类别:
Deciphering the GABA neuron alterations in schizophrenia
破译精神分裂症中 GABA 神经元的改变
- 批准号:
9890003 - 财政年份:2019
- 资助金额:
$ 37.04万 - 项目类别:
Deciphering the GABA neuron alterations in schizophrenia
破译精神分裂症中 GABA 神经元的改变
- 批准号:
10323264 - 财政年份:2019
- 资助金额:
$ 37.04万 - 项目类别:
Deciphering the GABA neuron alterations in schizophrenia
破译精神分裂症中 GABA 神经元的改变
- 批准号:
10545717 - 财政年份:2019
- 资助金额:
$ 37.04万 - 项目类别:
Chandelier cell inputs to pyramidal neurons in schizophrenia and development
精神分裂症和发育过程中吊灯细胞对锥体神经元的输入
- 批准号:
8439885 - 财政年份:2012
- 资助金额:
$ 37.04万 - 项目类别:
Chandelier cell inputs to pyramidal neurons in schizophrenia and development
精神分裂症和发育过程中吊灯细胞对锥体神经元的输入
- 批准号:
8690618 - 财政年份:2012
- 资助金额:
$ 37.04万 - 项目类别:
Chandelier cell inputs to pyramidal neurons in schizophrenia and development
精神分裂症和发育过程中吊灯细胞对锥体神经元的输入
- 批准号:
8538506 - 财政年份:2012
- 资助金额:
$ 37.04万 - 项目类别:
Chandelier cell inputs to pyramidal neurons in schizophrenia and development
精神分裂症和发育过程中吊灯细胞对锥体神经元的输入
- 批准号:
8879213 - 财政年份:2012
- 资助金额:
$ 37.04万 - 项目类别:
Differential Terminal Expression of GAD67/GAD65 ? Relevance to Schizophrenia
GAD67/GAD65 的差异末端表达?
- 批准号:
7569786 - 财政年份:2009
- 资助金额:
$ 37.04万 - 项目类别:
Differential Terminal Expression of GAD67/GAD65 ? Relevance to Schizophrenia
GAD67/GAD65 的差异末端表达?
- 批准号:
7802980 - 财政年份:2009
- 资助金额:
$ 37.04万 - 项目类别:
相似海外基金
Linking Epidermis and Mesophyll Signalling. Anatomy and Impact in Photosynthesis.
连接表皮和叶肉信号传导。
- 批准号:
EP/Z000882/1 - 财政年份:2024
- 资助金额:
$ 37.04万 - 项目类别:
Fellowship
Digging Deeper with AI: Canada-UK-US Partnership for Next-generation Plant Root Anatomy Segmentation
利用人工智能进行更深入的挖掘:加拿大、英国、美国合作开发下一代植物根部解剖分割
- 批准号:
BB/Y513908/1 - 财政年份:2024
- 资助金额:
$ 37.04万 - 项目类别:
Research Grant
Simultaneous development of direct-view and video laryngoscopes based on the anatomy and physiology of the newborn
根据新生儿解剖生理同步开发直视喉镜和视频喉镜
- 批准号:
23K11917 - 财政年份:2023
- 资助金额:
$ 37.04万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Genetics of Extreme Phenotypes of OSA and Associated Upper Airway Anatomy
OSA 极端表型的遗传学及相关上呼吸道解剖学
- 批准号:
10555809 - 财政年份:2023
- 资助金额:
$ 37.04万 - 项目类别:
computational models and analysis of the retinal anatomy and potentially physiology
视网膜解剖学和潜在生理学的计算模型和分析
- 批准号:
2825967 - 财政年份:2023
- 资助金额:
$ 37.04万 - 项目类别:
Studentship
Computational comparative anatomy: Translating between species in neuroscience
计算比较解剖学:神经科学中物种之间的翻译
- 批准号:
BB/X013227/1 - 财政年份:2023
- 资助金额:
$ 37.04万 - 项目类别:
Research Grant
Doctoral Dissertation Research: Social and ecological influences on brain anatomy
博士论文研究:社会和生态对大脑解剖学的影响
- 批准号:
2235348 - 财政年份:2023
- 资助金额:
$ 37.04万 - 项目类别:
Standard Grant
Development of a novel visualization, labeling, communication and tracking engine for human anatomy.
开发一种新颖的人体解剖学可视化、标签、通信和跟踪引擎。
- 批准号:
10761060 - 财政年份:2023
- 资助金额:
$ 37.04万 - 项目类别:
Understanding the functional anatomy of nociceptive spinal output neurons
了解伤害性脊髓输出神经元的功能解剖结构
- 批准号:
10751126 - 财政年份:2023
- 资助金额:
$ 37.04万 - 项目类别:
The Anatomy of Online Reviews: Evidence from the Steam Store
在线评论剖析:来自 Steam 商店的证据
- 批准号:
2872725 - 财政年份:2023
- 资助金额:
$ 37.04万 - 项目类别:
Studentship














{{item.name}}会员




