Request for an Olympus VS200 slide scanner

索取奥林巴斯 VS200 玻片扫描仪

• 批准号: 10280468
• 负责人: KENNETH N FISH
• 金额: $ 37.04万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2022-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10280468
• 关键词: 3-Dimensional; Anatomy; Area; Biological; Brain; Brain region; Communities; Core Facility; Data; Devices; Electrons; Eye; Frustration; Funding; Genes; Home; Image; Image Analysis; Institution; Light; Location; Medical; Medical Research; Methods; Microscope; Modality; Molecular; National Institute of Mental Health; Neck; Neuraxis; Oils; Research; Research Personnel; Research Project Grants; Resolution; Scanning; Science; Slide; System; Time; Universities; Work; base; image guided; imaging system; instrument; medical schools; operation; optical imaging; protein distribution; protein expression

This request is for funds to purchase an Olympus VS200 slide scanner with a multislide feeder and a storage server to hold data generated by the system. This instrument is being requested by a group of NIMH funded investigators who have a significant need to scan many hundreds of slides at high resolution in both brightfield and fluorescent modalities. The device will be housed within the Center for Biologic Imaging (CBI) at the University of Pittsburgh School of Medicine. The mandate of this core facility is to provide access to a full range of light and electron optical, image analysis, and morphometric methods to all research groups within the University of Pittsburgh School of Medicine. To gain a full and quantitative appreciation of the distribution of proteins and active genes within the central nervous system it is no longer sufficient to scan by eye and collect snapshot images that guide science as this commonly introduces bias and is certainly not appropriate when protein expression is at low levels or expressed in very discrete locations throughout brain regions. Rather there is a pressing and prevalent need to analyze distributions in 3D, commonly over many sections and over large areas. The investigators in this proposal have been using the “home brew” solutions available within the CBI. While these devices may have the spatial and spectral sensitivity to meet the needs of the investigative team they need to be tended to throughout the imaging to select image regions, add oil or change slides and are also being used by the very large number of investigators who are actively working with the CBI systems. Therefore, there is a single prime reason for this application: to provide critically needed high quality, automated instrument time to the NIMH supported users of the Center for Biologic Imaging. Since the CBI commenced operations 28 years ago, it has become an integral part of the medical research community currently participating in research projects with more than 300 PHS funded groups within the medical area, as well as in PHS supported projects with investigators in other departments and at neighboring institutions. The NIMH funded user base within the institution continues to grow and its requirement for large area automated imaging continues to expand. The lack of available scanning time has become a major limiting factor in the utility of the CBI to this specific group of users which has led to considerable frustration, as progress in funded work is absolutely dependent on high quality quantitative molecular anatomy of brain sections. The acquisition of an automated slide scanning microscope specifically for this user group will be a tremendous addition to the activity and functionality of the center as it will alleviate access on pre-existing systems for other user groups and will help alleviate the current user bottle neck for high resolution slide scanning by our NIMH funded colleagues.

此申请是为购买Olympus VS200载玻片扫描仪（带多玻片进样器）提供资金 以及存储服务器，用于保存由所述系统生成的数据。一个团体要求使用这一工具， 的NIMH资助的研究人员有显着需要扫描数百个幻灯片在高 在明场和荧光模式下的分辨率。该器械将存放在中心内， 匹兹堡大学医学院的生物成像（CBI）。这一核心融资机制的任务 是提供全方位的光和电子光学，图像分析和形态测量 匹兹堡大学医学院的所有研究小组。 为了获得蛋白质和活性基因分布的全面和定量的评价， 中枢神经系统，它不再是足够的扫描眼睛和收集快照图像，引导 科学，因为这通常会引入偏见，当然是不合适的，当蛋白质表达是在 低水平或在整个大脑区域非常离散的位置表达。相反，有一个紧迫的 并且普遍需要在3D中分析分布，通常在许多截面和大面积上。 这项提案中的调查人员一直在使用CBI内部可用的“自制”解决方案。 虽然这些设备可以具有空间和光谱灵敏度以满足调查的需要， 团队需要在整个成像过程中对其进行护理，以选择图像区域、添加润滑油或更换载玻片 并且也被大量积极与CBI合作的调查人员使用 系统.因此，这种应用有一个主要原因：提供急需的高 质量，自动化仪器时间NIMH支持的生物成像中心的用户。 自28年前开始运作以来，CBI已成为医疗保健的一个组成部分。 目前参与研究项目的研究团体有300多个PHS资助团体 在医疗领域内，以及在PHS支持的项目与其他部门的调查人员， 在邻近的机构。 NIMH资助的用户群在机构内继续增长，其对大型 区域自动成像继续扩展。缺乏可用的扫描时间已成为一个主要的问题， CBI对这一特定用户群体的效用的限制因素，这导致了相当大的 挫折，因为资助工作的进展绝对依赖于高质量的定量分子 脑切片的解剖学购买一台自动载玻片扫描显微镜， 这一用户群将极大地增加中心的活动和功能，因为它将减轻 为其他用户组提供对现有系统的访问，这将有助于缓解当前的用户瓶颈 由NIMH资助的同事进行高分辨率幻灯片扫描。