Cytoplasmic mitochondrial dsRNA in pediatric Sjogren's syndrome

小儿干燥综合征中的细胞质线粒体 dsRNA

基本信息

批准号：
10287866
负责人：
SEUNGHEE CHA
金额：
$ 36.91万
依托单位：
UNIVERSITY OF FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-22 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10287866
关键词：
Address Adolescent Adult Affect Applications Grants Autoimmune Autoimmunity B-Cell Lymphomas Bacterial Infections Biological Biological Assay Biopsy Specimen CD14 gene Cell Differentiation process Cell Line Cell physiology Cells Child Childhood Clinical Research Coculture Techniques Collaborations Communication Complement Control Groups Cytoplasm Data Diagnosis Diagnostic Disease Double-Stranded RNA Elderly Female Gene Expression Gene Targeting Genes Gland Goals High Prevalence Immune Impairment Inflammation Institutes Interdisciplinary Study Interferons Interleukin-17 Investigation Knowledge Lacrimal gland structure Lead Lymphoma Mitochondria Mitochondrial RNA Mus Nature Nucleic Acids Pain Parotitis Particulate Pathogenesis Pathology Pathway interactions Patients Pediatric cohort Peripheral Peripheral Blood Mononuclear Cell Polyribonucleotide Nucleotidyltransferase Production Prognosis Property RNA Degradation RNA Helicase Recurrence Regulation Regulatory T-Lymphocyte Reporting Role Salivary Glands Signal Transduction Sjogren&apos s Syndrome Stains Swelling Tissues Transfection Transfer RNA United States National Institutes of Health Viral Virus Diseases autoimmune inflammation cohort effector T cell evidence base experimental study genetic signature helicase high risk in vitro Assay indexing innovation intercellular communication knock-down macrophage mitochondrial genome monocyte multidisciplinary novel novel therapeutics sensor single-cell RNA sequencing small hairpin RNA therapeutic target transcriptome sequencing transcriptomics

项目摘要

ABSTRACT Through our multidisciplinary clinical and research collaboration, we have recently discovered a rare cohort of children and adolescents who fulfill the Sjögren’s syndrome (SS) diagnostic criteria (pediatric SS, PSS). As autoimmune SS typically affects elderly females, whether PSS is a unique disease entity or a mere reflection of an early stage of SS is completely unknown. PSS is commonly misdiagnosed as infectious recurrent parotitis (RP), since glandular swelling and pain without a viral/bacterial infection occurs more frequently in PSS than in SS. The overall goal of this application is to characterize the pathology of robust target tissue inflammation in PSS with RP. Our recent, preliminary discovery by RNA-sequencing of SS monocytes revealed aberrant expression of mitochondrial-dsRNA (mtdsRNA) regulators such as SUV3 (ATP-dependent RNA helicase, suppressor of variegation 3), along with the type I IFN signature and a tendency of M1 polarization-associated genes. Consistently, SUV3 gene expression was downregulated in PSS monocytes, detected by RT-qPCR, and knockdown of SUV3 by shRNA transfection led to massive accumulation of mtdsRNA in a cell line. More interestingly, cytoplasmic accumulation of particulate dsRNA was detected in PSS and SS monocytes, which prompted us to ask fundamental questions regarding the nature of the detected dsRNA and its potential impact on monocytes and subsequent immune dysregulation in PSS. To address these questions, we propose our central hypothesis that aberrant mtdsRNA degradation by altered RNA helicase expression and activity elicits amplified priming sensitivity of PSS monocytes by mimicking a viral infection. Our two Specific Aims are: Aim 1. To characterize the nature of cytoplasmic dsRNA involving SUV3 in monocytes of PSS. We hypothesize that cytoplasmic accumulation of mtdsRNA by aberrant mtdsRNA degradation triggers type I IFN production in monocytes of PSS. Cytoplasmic dsRNA transfer and mitochondrial genome depletion will be applied to identify potential mitochondriopathies involving SUV3, which will be complemented by RNA-sequencing of purified monocytes. Aim2. To determine the impact of dsRNA- positive monocytes on macrophage polarization and regulatory T cell (Treg) conversion. We hypothesize that dsRNA and type I IFN signature in PSS monocytes confer propensity toward intensified M1polarization impacting T reg plasticity. Various in vitro assays, such as co-culture experiments and functional assays, will be carried out in parallel with scRNA-seq of PMBC and paired biopsy specimens to profile cellular communications between macrophage and Tregs in the target tissue, which we hypothesize to be influenced by cytoplasmic dsRNA in PSS monocytes. To our knowledge, this is the first study to delineate the properties and impact of cytoplasmic mtdsRNA on monocyte dysregulation and robust inflammation in RP, as well as the first investigation of PSS pathogenesis in the field. Our novel hypothesis involving mtRNA helicases will provide a new opportunity to fill the current knowledge gap.

抽象的通过我们的多学科临床和研究合作，我们最近发现了一群罕见的队列满足Sjögren综合征（SS）诊断标准（小儿SS，PSS）的儿童和青少年。作为自身免疫性SS通常会影响上古老的女性，无论PSS是独特的疾病实体还是仅仅反映 SS的早期阶段是完全未知的。 PSS通常被诊断为传染性复发性炎症（RP），由于没有病毒/细菌感染而没有病毒/细菌感染的腺体肿胀和疼痛比在SS中更频繁地发生。该应用的总体目标是表征RP中PSS中强大的靶组织注入的病理。我们最近通过SS单核细胞的RNA测序发现的初步发现表明了异常表达线粒体-DSRNA（MTDSRNA）调节剂，例如SUV3（ATP依赖性RNA解旋酶，抑制剂variegation 3），以及I型IFN特征和M1极化相关基因的趋势。一贯，suv3基因在PSS单核细胞中下调，由RT-QPCR检测到SHARNA敲低SUV3 转染导致MTDSRNA在细胞系中的大量积累。更有趣的是，细胞质积累在PSS和SS单核细胞中检测到颗粒DSRNA，这促使我们提出基本问题关于检测到的DSRNA的性质及其对单核细胞和随后免疫的潜在影响 PSS的失调。为了解决这些问题，我们提出了我们的中心假设，即异常mtdsrna 通过改变RNA解旋酶表达和活性的降解引起PSS单核细胞的启动敏感性通过模仿病毒感染。我们的两个具体目的是：目标1。表征细胞质的性质 DSRNA涉及PSS单核细胞中的SUV3。我们假设MTDSRNA的细胞质积累异常mtDSRNA降解触发PSS单核细胞中I型I型IFN产生。细胞质DSRNA转移和线粒体基因组的部署将用于确定涉及SUV3的潜在线粒体病，将通过纯化的单核细胞进行RNA测序完成。 AIM2。确定dsRNA-的影响巨噬细胞极化和调节性T细胞（Treg）转化的阳性单核细胞。我们假设这一点 dsRNA和I型IFN签名在PSS单核细胞会议中有望增强M1Polariatization T Rege 可塑性。各种体外评估，例如共培养实验和功能评估，将同时进行 PMBC的Scrna-seq和配对的活检标本与巨噬细胞和目标组织中的Tregs，我们假设该靶组织会受到PSS单核细胞中细胞质DSRNA的影响。据我们所知，这是描述细胞质mTDSRNA对单核细胞的特性和影响的第一项研究 RP中的失调和强大注射，以及PSS发病机理的首次投资。我们的涉及MTRNA解放酶的新型假设将为填补当前知识差距提供新的机会。