Research Core 1: Molecular Measures Core (RC1)

项目摘要-分子测量核心(MMC) 分子测量核心(MMC)提供集中的资源、分析工具和专业知识来 测量广泛的分子分析器，以支持Duke OAIC项目，以了解和 优化储备和韧性。MMC已成为支持公爵的不可或缺的资源核心 OAIC自2006年以来。在这个资金周期中，MMC将专注于分子分析，这些分析提供了 对细胞、组织或器官层面的弹性的洞察有望增加我们的理解 在整个人的层面上优化储备和适应能力的手段。MMC将提供分子 为大量研究教育组成部分(REC)学者提供分析、指导和咨询支持 项目、试点/探索性研究(PESS)和外部项目(EP)。这些项目检验了相关的假设 在人类、灵长类动物和体外实验中，对寿命、储备和弹性的潜在机制， 并涉及一系列患者和样本类型。MMC能够提供服务来测量 广泛的细胞、分子、生化和代谢因素。中最相关的核心服务 下一个资金周期将包括炎症的分子测量，靶向和非靶向代谢组学， 与长寿相关的miRNAs的表观遗传学测量，生物学年龄的分子测量，以及 胞外小泡。此外，MMC将进行一个开发项目(MMC-DP)，以开发新的 分析冠状病毒血清以评估先前SARS-CoV2感染作为SARS-CoV2修饰物的能力 MoTrPAC队列的一项子研究中的心肺和代谢储备(1-2年)。这款MMC-DP 验证的血清学检测将提供给杜克大学OAIC的研究人员和后者的项目 多年的资金投入。MMC还将使用衰老模型系统(在本供资期间开发) 测试干预措施，包括‘长寿miRNA’和细胞外小泡的模拟物和抑制剂，以促进 复原力(1-5岁)。此外，MMC将提供路径分析(使用独创性系统) 分子数据，以确定涉及储备和复原力的潜在生物网络。令人兴奋的关注 这个周期中的韧性提供了一个机会来探索关于衰老的最新理论，这些理论特别是 服从并适合于通过分子方法进行评估。分子和途径的应用 对这些项目的分析有望为外汇储备的基本机制提供客观的见解。 和韧性，以支持OAIC公爵的使命。

PROJECT SUMMARY – Molecular Measures Core (MMC) The Molecular Measures Core (MMC) provides a centralized resource, analytical tools and expertise to measure a broad array of molecular analytes in support of Duke OAIC projects in their work to understand and optimize reserve and resilience. The MMC has served as an integral Resource Core in support of the Duke OAIC since 2006. For this funding cycle, the MMC will focus on molecular analyses that provide mechanistic insights into resiliencies at the cellular, tissue or organ levels that are expected to increase our understanding of the means to optimize reserve and resilience at the whole person level. The MMC will provide molecular analytic, mentoring and consultative support for a host of Research Education Component (REC) Scholar Projects, Pilot/Exploratory Studies (PESs) and External Projects (EPs). These projects test hypotheses related to aging across the lifespan, mechanisms underlying reserve and resilience in human, primates and in vitro, and involve a range of patient and sample types. The MMC is capable of providing services to measure a broad array of cellular, molecular, biochemical and metabolic factors. The most pertinent Core services in the next funding cycle will include molecular measures of inflammation, targeted and untargeted metabolomics, epigenetic measures of miRNAs related to longevity, molecular measures of biological age, and analyses of extracellular vesicles. In addition, the MMC will conduct a Developmental Project (MMC-DP) to develop new capabilities for profiling coronavirus serologies to evaluate prior SARS-CoV2 infection as a modifier of cardiorespiratory and metabolic reserve in a substudy of the MoTrPAC cohort (Years 1-2). This MMC-DP validated serological testing will be made available to the Duke OAIC researchers and projects in the latter years of funding. The MMC will also use the senescent model system (developed in the current funding period) to test interventions including mimics and inhibitors of ‘longevity miRNA’ and extracellular vesicles to promote resilience (Years 1-5). In addition, the MMC will provide Pathways Analysis (using Ingenuity Systems) of molecular data to identify potential biological networks involved in reserve and resilience. The exciting focus on resilience in this cycle provides an opportunity to explore the latest theories on aging that are particularly amenable and suited to evaluation by molecular approaches. The application of molecular and pathways analyses to these projects are expected to provide objective insights into the mechanisms underlying reserve and resilience in support of the mission of the Duke OAIC.