Advanced Analytics Core

高级分析核心 项目摘要 高级分析（AA）核心提供三大类高级分析服务： 基因组学、免疫技术和EpCultures（新）。EpCultures是类器官或平面培养物， 从捐赠者或模型生物的肠道中提取。这些服务本质上是复杂的， 实验室独立进行的成本很高，需要高水平的技术培训。AA核心 一直专注于开发创新的做法，以减少进入这些尖端技术的障碍， 技术.虽然这些服务看起来是不同的，但由于共同的 管理专业知识、咨询实践和高效的商业模式实践。核心提供 服务成本大幅降低，周转时间非常快。AA核心是在指导下， Scott Magness博士被认为是单细胞基因组和功能测定的领导者。他 在肠道和结肠类器官和使用原代组织来源的自我更新单层培养方面的专业知识 来源于人类和其他模式生物。机管局的核心人员是训练有素的专业人员， 这些技术要求高的方法，并取得了AA核心国家认可的单细胞 测序服务。AA核心的具体目标是：1）提供最先进的单细胞测定， 更好地了解疾病/损伤过程和细胞水平分辨率的上皮再生。这些测定 包括荧光激活细胞分选（FACS）、单细胞（sc）RNAseq、DNAseq、miRNA表达 和单细胞Western印迹。2)提供用于检测生物分子的免疫分析技术 通过ELISA和基于珠的多重测定。通常，CGIBD成员使用这些测定来检测 与各种疾病状态相关的生物标志物，如IBD。3)提供与类器官和 上皮单层培养物（EpCultures）、定制类器官测定和EpCultures培训。总的来说， 它们是强大的新技术，提供了高度生理相关的离体模型来研究 影响胃肠道的各种健康状况。

ADVANCED ANALYTICS CORE PROJECT SUMMARY The Advanced Analytics (AA) Core provides three main classes of advanced analytics services: Single-cell genomics, Immunotechnologies, and EpCultures (new). EpCultures are organoid or planar cultures derived from the intestines of donor humans or model organisms. These services are sophisticated in nature, expensive for laboratories to independently perform, and require a high level of technical training. The AA Core has always focused on developing innovative practices to reduce barriers of entry to these cutting-edge technologies. While seemingly disparate, the services have been grouped together because of common management expertise, consultation practices, and efficient business model practices. The core provides services at substantially reduced costs and very rapid turnaround times. The AA Core is under the direction of Scott Magness, PhD, who is considered a leader in single-cell genomic and functional assays. He has expertise in intestinal and colonic organoid and self-renewing monolayer culture using primary tissue sources derived from humans and other model organisms. The AA Core personnel are highly trained professionals in these technically demanding methods, and have made the AA Core nationally recognized for single-cell sequencing services. The Specific Aims of the AA Core are: 1) To offer state-of-the-art single-cell assays to better understand disease/injury processes and epithelial regeneration at cell-level resolution. These assays include, Fluorescence Activated Cell Sorting (FACS), single-cell (sc) RNAseq, DNAseq, miRNA expression, and single-cell Western blotting. 2) To provide immunoassay technologies for the detection of biomolecules via ELISA and bead-based multiplex assay. Typically, CGIBD members use these assays to detect biomarkers associated with various disease states, such as IBD. 3) To offer services related to organoid and epithelial monolayer cultures (EpCultures), custom organoid assays, and training in EpCultures. Collectively, they are powerful new technologies that provide highly physiologically relevant ex vivo models to study a variety of health conditions that affect the GI tract.