Elucidating glial diversity in the peripheral nervous system

阐明周围神经系统中的神经胶质多样性

• 批准号: 10314960
• 负责人: Sarah C Kucenas
• 金额: $ 44.41万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-15 至 2023-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10314960
• 关键词: Ablation; Axon; Candidate Disease Gene; Cell Nucleus; Cells; Cephalic; Clustered Regularly Interspaced Short Palindromic Repeats; Color; Coupled; Coupling; Data; Development; Disease; Distal; Dorsal; Event; Fertilization; Genes; Goals; Hour; Injury; Larva; Lasers; Lead; Light; Mediating; Modeling; Molecular; Motor; Nerve; Nervous system structure; Neural Crest Cell; Neuroglia; Nuclear; Organism; Perineuriums; Peripheral; Peripheral Nerves; Peripheral Nervous System; Pharmacology; Population; Population Dynamics; Predisposition; Process; Resources; Role; Schwann Cells; Sensory; Small Nuclear RNA; Specific qualifier value; Spinal; Spinal Cord; Structure; System; Taxonomy; Time; Transgenic Organisms; Zebrafish; cell type; genetic information; genetic signature; glial cell development; imaging genetics; in vivo; migration; mutant; nervous system development; novel; oligodendrocyte progenitor; stem cells; tool; transcriptome sequencing

Peripheral nerve assembly requires the coordinated development of many components, including axons and the glial cells that ensheath them. Currently, we have a broad perspective of the structure of peripheral nerves and the events that govern nerve assembly. However, we still lack a complete picture of all of the cellular and molecular mechanisms that mediate these events, and whether perturbations to these processes underlie disease. This is in part due to the fact that we still don’t have a full appreciation of the diversity of glial populations that make up these structures and there are currently no specific markers for any peripheral glial population. Additionally, although peripheral nerves are often grouped together as a single, uniform structure, there are in fact many types, including cranial, trunk, sensory, and motor, as well as spatial organization along the proximal-distal axis. However, whether there is any glial diversity between or along these structures, and whether this diversity is what contributes to the differences observed between these nerves with respect to function and susceptibility to disease, has yet to be determined. Using single-nuclei RNA-sequencing coupled with the powerful system, zebrafish, we will create a molecular taxonomy of peripheral glial subpopulations and decipher their roles in nervous system development.

周围神经组装需要许多组件的协调发展，包括轴突和神经元。 包裹它们的神经胶质细胞。目前，我们对周围神经的结构有了广泛的认识， 控制神经组装的事件。然而，我们仍然缺乏一个完整的图片，所有的细胞和 介导这些事件的分子机制，以及这些过程的扰动是否 疾病这部分是由于我们仍然没有充分认识到神经胶质细胞群的多样性 目前还没有任何特定的标记物来标记任何周围神经胶质细胞群。 此外，尽管周围神经通常被分组在一起作为单个的、统一的结构，但是在周围神经中， 事实上有许多类型，包括颅、躯干、感觉和运动，以及沿近端-远端轴沿着的空间组织。然而，在这些结构之间或沿着这些结构是否有任何胶质细胞的多样性，以及这是否 多样性是导致这些神经之间在功能方面观察到的差异的原因， 对疾病的敏感性尚未确定。使用单核RNA测序结合 强大的系统，斑马鱼，我们将创建一个外周神经胶质细胞亚群的分子分类，并破译 在神经系统发育中的作用

项目成果

Cd59 and inflammation regulate Schwann cell development.

• DOI: 10.7554/elife.76640
• 发表时间: 2022-06-24
• 期刊: ELIFE
• 影响因子: 7.7
• 作者: Wiltbank, Ashtyn T.;Steisnon, Emma R.;Criswell, Stacey J.;Piller, Melanie;Kucenas, Sarah
• 通讯作者: Kucenas, Sarah

Glutamate Signaling via the AMPAR Subunit GluR4 Regulates Oligodendrocyte Progenitor Cell Migration in the Developing Spinal Cord.

通过 AMPAR 亚基 GluR4 的谷氨酸信号传导调节发育中脊髓中的少突胶质细胞祖细胞迁移。

• DOI: 10.1523/jneurosci.2562-20.2021
• 发表时间: 2021
• 期刊: The Journal of neuroscience : the official journal of the Society for Neuroscience
• 作者: Piller,Melanie;Werkman,IngeL;Brown,RobinIsadora;Latimer,AndrewJ;Kucenas,Sarah
• 通讯作者: Kucenas,Sarah