Elucidating glial diversity in the peripheral nervous system

周围神经的组装需要许多组成部分的协调发展，包括轴突和 包裹它们的神经胶质细胞。目前，我们对周围神经的结构和 支配神经组装的事件。然而，我们仍然缺乏对所有细胞和 调节这些事件的分子机制，以及对这些过程的扰动是否是基础 疾病。这在一定程度上是因为我们仍然没有充分认识到神经胶质种群的多样性 它们构成了这些结构，目前还没有针对任何外周神经胶质细胞群体的特定标志物。 此外，尽管周围神经通常被组合在一起作为一个单一的、统一的结构，但在 事实上，包括头颅、躯干、感觉和运动在内的多种类型，以及沿近远轴线的空间组织。然而，在这些结构之间或沿这些结构是否存在胶质多样性，以及这是否 多样性是这些神经在功能和功能方面观察到的差异的原因 对疾病的易感性还有待确定。使用单核RNA测序与 强大的系统，斑马鱼，我们将创建一个外周神经胶质亚群的分子分类并破译 它们在神经系统发育中的作用。

Peripheral nerve assembly requires the coordinated development of many components, including axons and the glial cells that ensheath them. Currently, we have a broad perspective of the structure of peripheral nerves and the events that govern nerve assembly. However, we still lack a complete picture of all of the cellular and molecular mechanisms that mediate these events, and whether perturbations to these processes underlie disease. This is in part due to the fact that we still don’t have a full appreciation of the diversity of glial populations that make up these structures and there are currently no specific markers for any peripheral glial population. Additionally, although peripheral nerves are often grouped together as a single, uniform structure, there are in fact many types, including cranial, trunk, sensory, and motor, as well as spatial organization along the proximal-distal axis. However, whether there is any glial diversity between or along these structures, and whether this diversity is what contributes to the differences observed between these nerves with respect to function and susceptibility to disease, has yet to be determined. Using single-nuclei RNA-sequencing coupled with the powerful system, zebrafish, we will create a molecular taxonomy of peripheral glial subpopulations and decipher their roles in nervous system development.