UC Irvine AD Translational Center for Disease Model Resources-NextSeq Supplement

项目摘要/摘要 UCI模型-AD疾病模型开发和表型项目(DMDPP)的一个关键目标是 表征新的散发性阿尔茨海默病啮齿动物模型的基因表达水平 老化过程中的财团。这包括为Model-Seq建立大量的批量和单细胞RNA-seq文库。 广告小鼠。所有这些文库目前都是在2013年购买的Illumina NextSeq 500上测序的。因为这就是 仪器即将由Illumina停产支持，我们正在申请资金来升级Illumina NextSeq 2000系统，以提高批量和单细胞rna-seq测序的可靠性和吞吐量。 文库由UCI Model-AD制作。这次升级将确保及时对rna-seq文库进行测序。 UCI模型-AD鼠模型衰老过程中基因表达变化的特征。

Project Summary/Abstract A key goal of the UCI Model-AD Disease Model Development and Phenotyping Project (DMDPP) is to characterize the gene expression levels of the new rodent models of sporadic AD being generated by the consortium during aging. This involves the building of numerous bulk and single-cell RNA-seq libraries for Model- AD mice. All of these libraries are currently sequenced on an Illumina NextSeq 500 purchased in 2013. As this instrument is nearing its end-of-life support by Illumina, we are requesting funds to upgrade the Illumina NextSeq 2000 system in order to increase the reliability and throughput of sequencing the bulk and single-cell RNA-seq libraries produced by UCI Model-AD. The upgrade will ensure the timely sequencing of the RNA-seq libraries for the characterization of the changes of gene expression during aging in the UCI model-AD rodent models.