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Mouse Genetics

小鼠遗传学

基本信息

批准号：
10319508
负责人：
YASUHIDE FURUTA
金额：
$ 100.26万
依托单位：
SLOAN-KETTERING INST CAN RESEARCH
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-01-20 至 2023-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10319508
关键词：
Alleles Animals CRISPR/Cas technology Cancer Biology Cancer Center Support Grant Cell Culture Techniques Clustered Regularly Interspaced Short Palindromic Repeats Communities Complement Consultations Cryopreservation DNA DNA purification Derivation procedure Development Electroporation Embryo Transfer Embryology Embryonic Development Gene Targeting Generations Genes Genetic Genetic Engineering Genetically Engineered Mouse Genotype Germ-Line Mutation Guide RNA Immunobiology Injections Investigational Therapies Malignant Neoplasms Methods Microinjections Mission Molecular Biology Molecular Genetics Mouse Strains Mus Operative Surgical Procedures Performance Physiological Pre-Clinical Model Production Productivity Reagent Recovery Research Personnel Role Services Technology Testing Time Training Transgenes Transgenic Organisms Translational Research Tumor Suppressor Genes anticancer research base blastocyst cell behavior cell growth design embryo cryopreservation embryonic stem cell gene function genome editing genome integrity genome sequencing human model in vivo mouse genetics mouse genome mouse model novel novel therapeutic intervention reconstitution sperm cell stem cell biology tissue culture tool transcription activator-like effector nucleases tumorigenesis zygote

项目摘要

ABSTRACT The primary role of the Mouse Genetics Core is to facilitate the use of mouse molecular genetics at MSK for in vivo studies of gene functions germane to cancer. Relevant fields in which MSK investigators develop and apply mouse models include: cell growth and behavior, stem cell biology, embryonic development, immunobiology, genome integrity, cancer biology, and experimental therapeutics. The MGC consists of 2 sections: the Transgenic/Embryology Group (TgG) and the Molecular Biology/Tissue Culture Group (MBTCG). Major services offered by the TgG include the production of genetically engineered mice (GEM) by pronuclear injection of DNA or CRISPR/Cas9 and by blastocyst injection of ES cells; cryopreservation and the long-term storage of GEM lines; strain rederivation or recovery through IVF and/or embryo transfer; performance of special embryological and animal surgical procedures; and the provision of GEM lines such as CRE and FLPe expressing strains. The specialized molecular biology services provided by the MBTCG include transgene DNA purification and genotyping of founder mice; the design and production of CRISPR/Cas9 reagents for mouse genome editing; and the identification and verification of alleles carried by gene edited mice. Services offered by the MBTCG also include performing all aspects of gene targeting in mouse ES cells and establishing ES cells from GEM lines. An integral role of the MGC is to provide consultation and training in the design and production of mouse models, ES cell culture, and mouse husbandry and genetics. Rapid advances in genome editing and sequencing technologies, in combination with the steady increase in translational research at MSK, have resulted in a significant upsurge in demand for MGC services. In addition, the Core continues to pursue and implement new technical developments that will expedite gene editing in mice; ongoing efforts include the use of commercially available synthetic gRNA components and the application of electroporation to deliver CRIPSR/Cas9 into mouse zygotes. Integrating such advancements into the Core’s services will undoubtedly increase the productivity and reduce staff time/effort to create GEM models for MSK investigators.

摘要