Mouse Genetics

小鼠遗传学

• 批准号: 10084827
• 负责人: YASUHIDE FURUTA
• 金额: $ 100.26万
• 依托单位: SLOAN-KETTERING INST CAN RESEARCH
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-20 至 2023-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10084827
• 关键词: Alleles; Animals; CRISPR/Cas technology; Cancer Biology; Cancer Center Support Grant; Cell Culture Techniques; Clustered Regularly Interspaced Short Palindromic Repeats; Communities; Complement; Consultations; Cryopreservation; DNA; DNA purification; Derivation procedure; Development; Electroporation; Embryo Transfer; Embryology; Embryonic Development; Gene Targeting; Generations; Genes; Genetic; Genetic Engineering; Genetically Engineered Mouse; Genotype; Germ-Line Mutation; Guide RNA; Immunobiology; Injections; Investigational Therapies; Malignant Neoplasms; Methods; Microinjections; Mission; Molecular Biology; Molecular Genetics; Mouse Strains; Mus; Operative Surgical Procedures; Performance; Physiological; Pre-Clinical Model; Production; Productivity; Reagent; Recovery; Research Personnel; Role; Services; Technology; Testing; Time; Training; Transgenes; Transgenic Organisms; Translational Research; Tumor Suppressor Genes; anticancer research; base; blastocyst; cell behavior; cell growth; design; embryo cryopreservation; embryonic stem cell; gene function; genome editing; genome integrity; genome sequencing; human model; in vivo; mouse genetics; mouse genome; mouse model; novel; novel therapeutic intervention; reconstitution; sperm cell; stem cell biology; tissue culture; tool; transcription activator-like effector nucleases; tumorigenesis; zygote

ABSTRACT The primary role of the Mouse Genetics Core is to facilitate the use of mouse molecular genetics at MSK for in vivo studies of gene functions germane to cancer. Relevant fields in which MSK investigators develop and apply mouse models include: cell growth and behavior, stem cell biology, embryonic development, immunobiology, genome integrity, cancer biology, and experimental therapeutics. The MGC consists of 2 sections: the Transgenic/Embryology Group (TgG) and the Molecular Biology/Tissue Culture Group (MBTCG). Major services offered by the TgG include the production of genetically engineered mice (GEM) by pronuclear injection of DNA or CRISPR/Cas9 and by blastocyst injection of ES cells; cryopreservation and the long-term storage of GEM lines; strain rederivation or recovery through IVF and/or embryo transfer; performance of special embryological and animal surgical procedures; and the provision of GEM lines such as CRE and FLPe expressing strains. The specialized molecular biology services provided by the MBTCG include transgene DNA purification and genotyping of founder mice; the design and production of CRISPR/Cas9 reagents for mouse genome editing; and the identification and verification of alleles carried by gene edited mice. Services offered by the MBTCG also include performing all aspects of gene targeting in mouse ES cells and establishing ES cells from GEM lines. An integral role of the MGC is to provide consultation and training in the design and production of mouse models, ES cell culture, and mouse husbandry and genetics. Rapid advances in genome editing and sequencing technologies, in combination with the steady increase in translational research at MSK, have resulted in a significant upsurge in demand for MGC services. In addition, the Core continues to pursue and implement new technical developments that will expedite gene editing in mice; ongoing efforts include the use of commercially available synthetic gRNA components and the application of electroporation to deliver CRIPSR/Cas9 into mouse zygotes. Integrating such advancements into the Core’s services will undoubtedly increase the productivity and reduce staff time/effort to create GEM models for MSK investigators.

抽象的 小鼠遗传学核心的主要作用是促进小鼠分子遗传学在 MSK 的应用 与癌症密切相关的基因功能的体内研究。 MSK 斯隆研究人员开发和开发的相关领域 应用小鼠模型包括：细胞生长和行为、干细胞生物学、胚胎发育、 免疫生物学、基因组完整性、癌症生物学和实验疗法。 MGC 由 2 部门：转基因/胚胎学组（TgG）和分子生物学/组织培养组（MBTCG）。 TgG提供的主要服务包括通过原核技术生产基因工程小鼠（GEM） 注射 DNA 或 CRISPR/Cas9 以及通过胚泡注射 ES 细胞；冷冻保存和长期保存 GEM线的存储；通过 IVF 和/或胚胎移植进行菌株再衍生或恢复；的表现 特殊的胚胎学和动物外科手术；以及提供CRE和FLPe等创业板线路 表达菌株。 MBTCG 提供的专业分子生物学服务包括转基因 始祖小鼠的 DNA 纯化和基因分型； CRISPR/Cas9试剂的设计和生产 小鼠基因组编辑；以及基因编辑小鼠携带的等位基因的鉴定和验证。服务 MBTCG 提供的服务还包括在小鼠 ES 细胞中进行基因打靶的各个方面， 从 GEM 系建立 ES 细胞。 MGC 的一个不可或缺的作用是提供咨询和培训 小鼠模型的设计和制作、胚胎干细胞培养以及小鼠饲养和遗传学。快速进步 基因组编辑和测序技术的发展，以及转化技术的稳步增长 MSK 斯隆的研究导致对 MGC 服务的需求大幅增加。此外，核心 继续追求和实施新技术开发，以加快小鼠基因编辑； 正在进行的努力包括使用市售的合成 gRNA 成分以及应用 电穿孔将 CRIPSR/Cas9 递送至小鼠受精卵中。将这些进步集成到核心中 服务无疑将提高生产力并减少员工为 MSK 创建 GEM 模型的时间/精力 调查人员。