Versatile femtosecond technology for adaptive multi-photon imaging

用于自适应多光子成像的多功能飞秒技术

• 批准号: 10321235
• 负责人: William Renninger
• 金额: $ 34.48万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-02-01 至 2023-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10321235
• 关键词: Animals; Artificial Heart; Attention; Biomedical Research; Biomedical Technology; Biosensing Techniques; Brain; Brain imaging; Cataract; Complex; DNA; Development; Embryology; Fiber; Generations; Goals; Image; Immunology; Lasers; Light; Microscope; Mus; Neurons; Neurophysiology - biologic function; Neurosciences; Optics; Pattern; Performance; Phase; Physiologic pulse; Power Sources; Process; Pump; Research; Residual state; Risk; Scheme; Seeds; Source; Spectrum Analysis; Speed; System; Techniques; Technology; Time; Tissue imaging; Training; Transfection; absorption; anticancer research; awake; base; cardiac tissue engineering; chemotherapy; corneal surgery; cost; design; experimental study; improved; innovation; multi-photon; multiphoton imaging; multiphoton microscopy; novel; operation; programs; success; three photon microscopy; two-photon

Abstract The goal of this research program is to develop femtosecond source technologies that move beyond mode- locked lasers and to demonstrate these new techniques with multiphoton microscopy based on adaptive excitation. High speed multiphoton imaging based on adaptive excitation requires wavelength and pulse pattern agility that is not currently available. Traditional laser-based sources, moreover, are limited in wavelength, repetition rate, complexity, and cost. The research proposed will first demonstrate the desired versatile femtosecond source and then apply this source to multiphoton microscopy based on adaptive excitation. The research program is based on three major innovations: (1) high quality femtosecond pulse trains can be generated through optical modulation and novel nonlinear pulse compression and cleaning techniques, (2) high energy wavelength-agile amplification can be achieved in fiber through broadband intra-pulse phase matching of chirped pulses, and (3) adaptive excitation enables more than order-of- magnitude improvements to the speed of multiphoton microscopy with a high power source supporting the combination of an arbitrary wavelength and pulse pattern, such as with the proposed robust fiber-format technique enabled by innovations (1) and (2). Our aim is to enable more than an order-of-magnitude improvement to frame rates at the ultimate depth limits to imaging with a novel source that is significantly more accessible than previous technologies. Successful completion of this program will have major impact for biomedical research directions which employ ultrashort pulse technology, such as deep tissue imaging.

抽象的 该研究计划的目的是开发超越模式的飞秒源技术 - 锁定激光器，并以自适应为基础的多光子显微镜演示这些新技术 励磁。基于自适应激发的高速多光子成像需要波长和脉冲 当前尚不可用的模式敏捷性。此外，传统的基于激光的来源受到限制 波长，重复率，复杂性和成本。提出的研究将首先证明所需的 多功能飞秒源，然后将此源应用于基于自适应的多光子显微镜 励磁。该研究计划基于三个主要创新：（1）高质量飞秒脉冲 可以通过光学调制和新型的非线性脉冲压缩和清洁生成火车 技术，（2）可以通过宽带在纤维中实现高能波长敏捷的扩增 pul脉脉冲的脉冲脉冲匹配，（3）自适应激发比 - 不仅仅是 - 高电源支持的多光子显微镜速度的幅度提高了 任意波长和脉冲图案的组合，例如与提出的稳健纤维形式的结合 创新（1）和（2）启用了技术。我们的目的是启用不仅仅是设施命令 以最终深度限制对帧速率的提高，以具有明显的新来源成像 比以前的技术更容易访问。成功完成该计划将产生重大影响 用于使用超短脉冲技术的生物医学研究方向，例如深层组织成像。