权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

MOLECULAR ANALYSIS CELLULAR IMAGING (MACI) CORE

分子分析细胞成像 (MACI) 核心

基本信息

批准号：
10320857
负责人：
WILLARD M FREEMAN
金额：
$ 37.95万
依托单位：
UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-02-01 至 2023-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10320857
关键词：
Aging Animal Model Applications Grants Area Binding Biochemical Bioinformatics Biological Cells ChIP-seq Chromatin Complement Consultations Core Facility Creativeness DNA Methylation DNA-Binding Proteins Data Data Analyses Data Set Detection Development Disease Epigenetic Process Experimental Designs Fostering Gene Expression Gene Expression Profiling Gene Expression Regulation Gene Proteins Genes Geroscience Goals Growth Health Health Sciences Heterogeneity Human Resources Image Image Analysis Imaging technology Individual Investigation Label Laboratories Light Link Microscopy Mitochondria Modality Molecular Molecular Analysis Nucleic Acids Oklahoma Pathway interactions Preparation Process Program Development Protein Isoforms Proteins Publishing Regulation Research Research Design Research Personnel Resources Role Scientist Services Shock Structure Tissue Sample Training Training Technics Transcript Transmission Electron Microscopy age related cellular imaging design epigenetic regulation experience heteroplasmy imaging approach imaging capabilities imaging study innovation insight instrumentation interest knowledge base molecular imaging programs protein expression recruit single-cell RNA sequencing skills tissue preparation tool transcription factor transcriptome transcriptome sequencing

项目摘要

Basic Geroscience research requires the analysis of gene and protein regulation, expression, and cellular localization. Advanced molecular analyses and imaging technologies offer investigators a powerful set of tools for their studies, but the instrumentation, experience, and skills to fully exploit these tools are often far beyond the capability of a single laboratory. The Molecular Analysis and Cellular Imaging (MACI) Core will provide a range of instrumentation, services, and training for rigorous analysis of structure and function through gene expression, gene regulation, and imaging studies. In this way, the MACI Core will advance understanding of molecular and cellular age-related changes in health and disease. The MACI Core will employ personnel with extensive scientific experience in these areas who already provide collaborative assistance to individual investigators and have significant experience operating core resources. The MACI Core will offer a range of technical services, and also expert advice in study design, experimental approaches, data analysis and interpretation, grant proposal preparation and training. The MACI Core will provide advanced molecular analyses of gene expression and regulation (Aim 1) including high throughput single-cell RNA-Seq (HT-scRNA- Seq) strand-specific RNA-Seq gene expression, high-throughput qPCR, chromatin immunoprecipitation sequencing (ChIP-Seq) for DNA binding proteins. Aim 2 of the MACI Core is to provide advanced, application-specific tissue preparation, cellular labeling, imaging, and image analysis services and training. For cellular localization studies, the MACI Core will offer tissue preparation and sectioning along with light, confocal, epifluorescence, and transmission electron microscopy services. A key service will be to help our PJIs and other investigators to develop advanced, lab-specific cellular labeling and imaging approaches. Aim 3 of the MACI Core is to provide training and advanced molecular and cellular data analysis services. Ranging from nucleic acid isolation and tissue preparation and sectioning to bioinformatics and image analysis, a combination of bimonthly training seminars and project-specific training will expand the knowledge base and capabilities of Geroscience researchers. Consultation on experimental design, analysis, and grant proposals will also be provided. The MACI Core, along with partner Cores, will aid the development and continued growth of Geroscience research and foster independence, innovation, and creativity of the individual research programs.

基础基因科学研究需要分析基因和蛋白质的调控、表达和细胞