MOLECULAR ANALYSIS CELLULAR IMAGING (MACI) CORE

基础老年学研究需要分析基因和蛋白质的调节、表达和细胞 本地化。先进的分子分析和成像技术为研究人员提供了一套强大的工具 但充分利用这些工具的仪器、经验和技能往往远远超出 单个实验室的能力。分子分析和细胞成像(MACI)核心将提供 通过基因对结构和功能进行严格分析的一系列仪器、服务和培训 表达、基因调控和成像研究。通过这种方式，MACI核心将促进对 健康和疾病中与分子和细胞年龄相关的变化。MACI核心将聘用以下人员 在这些领域有丰富的科学经验，已经为个人提供协作帮助 调查人员，并拥有运营核心资源的丰富经验。Maci Core将提供一系列 技术服务，以及研究设计、实验方法、数据分析和 口译、资助金提案准备和培训。MACI核心将提供先进的分子 基因表达与调控分析(目标1)，包括高通量单细胞RNA-Seq(HT-scRNA-Seq) Sq)链特异性RNA-Seq基因表达、高通量qPCR、染色质免疫沉淀测序 (ChIP-Seq)用于DNA结合蛋白。MACI核心的目标2是提供先进的、特定于应用的组织 准备、细胞标记、成像和图像分析服务和培训。对于蜂窝定位研究， MACI核心将提供组织准备和切片以及光、共焦、荧光和 提供透射电子显微镜服务。一项关键服务将是帮助我们的PJI和其他调查人员 开发先进的、实验室专用的细胞标记和成像方法。MACI核心的目标3是提供 培训和先进的分子和细胞数据分析服务。从核酸分离到 组织准备和切片到生物信息学和图像分析，双月培训相结合 研讨会和具体项目培训将扩大老年科学的知识基础和能力 研究人员。还将提供关于实验设计、分析和拨款建议的咨询。这个 MACI核心和合作伙伴核心将帮助老年科学研究的发展和持续增长 培养个人研究项目的独立性、创新性和创造性。

Basic Geroscience research requires the analysis of gene and protein regulation, expression, and cellular localization. Advanced molecular analyses and imaging technologies offer investigators a powerful set of tools for their studies, but the instrumentation, experience, and skills to fully exploit these tools are often far beyond the capability of a single laboratory. The Molecular Analysis and Cellular Imaging (MACI) Core will provide a range of instrumentation, services, and training for rigorous analysis of structure and function through gene expression, gene regulation, and imaging studies. In this way, the MACI Core will advance understanding of molecular and cellular age-related changes in health and disease. The MACI Core will employ personnel with extensive scientific experience in these areas who already provide collaborative assistance to individual investigators and have significant experience operating core resources. The MACI Core will offer a range of technical services, and also expert advice in study design, experimental approaches, data analysis and interpretation, grant proposal preparation and training. The MACI Core will provide advanced molecular analyses of gene expression and regulation (Aim 1) including high throughput single-cell RNA-Seq (HT-scRNA- Seq) strand-specific RNA-Seq gene expression, high-throughput qPCR, chromatin immunoprecipitation sequencing (ChIP-Seq) for DNA binding proteins. Aim 2 of the MACI Core is to provide advanced, application-specific tissue preparation, cellular labeling, imaging, and image analysis services and training. For cellular localization studies, the MACI Core will offer tissue preparation and sectioning along with light, confocal, epifluorescence, and transmission electron microscopy services. A key service will be to help our PJIs and other investigators to develop advanced, lab-specific cellular labeling and imaging approaches. Aim 3 of the MACI Core is to provide training and advanced molecular and cellular data analysis services. Ranging from nucleic acid isolation and tissue preparation and sectioning to bioinformatics and image analysis, a combination of bimonthly training seminars and project-specific training will expand the knowledge base and capabilities of Geroscience researchers. Consultation on experimental design, analysis, and grant proposals will also be provided. The MACI Core, along with partner Cores, will aid the development and continued growth of Geroscience research and foster independence, innovation, and creativity of the individual research programs.